ORIGINAL ARTICLE
Nanoactivities of natural nanomaterials rosmarinic acid, glycyrrhizic acid and glycyrrhizic acid ammonium salt against tomato phytopathogenic fungi Alternaria alternata and Penicillium digitatum
 
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1
Post Harvest Department, Plant Pathology Institute, Agriculture Research Centre, Giza, Egypt
2
Environmental Biotechnology, Misr University for Science and Technology, College of Biotechnology, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Tahsin Mansour Shoala   

Environmental Biotechnology Department, College of Biotechnology, Misr University for Science and Technology, 6th of October City, Egypt
Online publication date: 2020-05-26
Submission date: 2019-07-18
Acceptance date: 2019-10-14
 
Journal of Plant Protection Research 2020;60(2):150–160
KEYWORDS
TOPICS
ABSTRACT
Black mold and green mold caused by Alternaria alternata and Penicillium digitatum, respectively, are the most important decay pathogens of tomato fruits during storage. Our research was aimed to control tomato phytopathogenic fungi A. alternata and P. digitatum in vitro and in vivo by using natural nanomaterials rosmarinic acid (RA-NPs) at concentrations of 0.3 and 0.6 mM, glycyrrhizic acid (GA-NPs) and glycyrrhizic acid ammounium salt (GAS-NPs) (0.1–0.2 mM). Characterizations of the tested nanoparticles were carried out by using dynamic light scattering which revealed that synthesized nanoparticles had particle sizes of less than 100 nm. In vitro studies revealed that the three tested nanoparticles reduced the growth of A. alternata and P. digitatum. Glycyrrhizic acid nanoparticles were the most effective in reducing the growth of the two tested pathogens followed by RA-NPs at 0.6 mM. Observations of A. alternata and P. digitatum by scanning electron microscopy (SEM) showed severe damage in the hyphae and deformities in the conidia due to the effect of the tested nanoparticles. In vivo results showed that, dipping tomato fruits as a post-harvest treatment in all of the tested nanoparticles at different concentrations, then stored at 10 ± 1°C and 90–95% relative humidity (RH) for 20 days greatly reduced the disease severity of infected fruits with the two tested pathogens. GA-NPs at 0.2 mM significantly reduced the development of black mold rot on tomato fruits. RA-NPs at 0.6 mM had the best effect in controlling P. digitatum of all naturally and artificially inoculated tomato fruits. Also, individual treatments of tomato fruits with RA-NPs, GA-NPs and GAS-NPs significantly reduced postharvest losses of fruit since they delayed decay and maintained fruit quality characteristics such as fruit firmness, titratable acidity and total soluble solids during cold storage.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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