ORIGINAL ARTICLE
Characterization of Paramyrothecium roridum (Basionym Myrothecium roridum) causing leaf spot of strawberry
1
Mycology Research and Disease Survey Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2019-12-07
Acceptance date: 2020-02-03
Online publication date: 2020-05-26
Corresponding author
Maali Shaker Soliman
Mycology Research and Disease Survey Department, Plant Pathology Research Institute, Agricultural Research Center, 9 Gamaa street, 12211 Giza, Egypt
Mycology Research and Disease Survey Department, Plant Pathology Research Institute, Agricultural Research Center, 9 Gamaa street, 12211 Giza, Egypt
Journal of Plant Protection Research 2020;60(2):141-149
KEYWORDS
TOPICS
ABSTRACT
Strawberry plants showing symptoms of leaf spots and petiole lesions were collected from
El Qalubya governorate, which is one of the most famous areas that extensively grows
strawberry in Egypt. The objectives of this study were to isolate and characterize the causal
pathogen of the disease. The isolated pathogen was identified as Paramyrothecium roridum
(formerly known as Myrothecium roridum) based on its morphological characteristics and
sequencing the partial rDNA internal transcribed spacer (ITS). A pathogenicity test using
detached leaf assay revealed that P. roridum is a potential pathogen of strawberry. Symptoms
started as small necrotic areas which expanded rapidly to macerate whole leaflets
and petioles. In advanced stages of infection, dark olive green sporodochia were clearly
distinguished on the infected tissues. Six strawberry cultivars showed different levels of
susceptibility to P. roridum. Florida was the most resistant cultivar while Beauty, Camarosa,
Fortuna and Sweet Charlie were susceptible. Festival showed a moderate level of susceptibility.
An in vitro assay on the effect of the liquid culture filtrate of P. roridum on strawberry
leaves showed that the filtrate caused damage to tissues and clear necrotic symptoms
were developed. High performance liquid chromatograph (HPLC) analysis on the filtrate of
10 day old P. roridum culture revealed the presence of various mycotoxins. The two major
toxins detected were 8-alpha-hydroxyroridin H and myrothecin A in addition to other trichothecenes.
Data also revealed the capability of P. roridum to produce polygalacturonase
(PG) and cellulase (Cx) enzymes in liquid cultures. The activity of PG was found to be significantly
correlated with the age of the growth culture. This is the first record of P. roridum
on strawberry in Egypt.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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