ORIGINAL ARTICLE
Pathological and molecular variation in Colletotrichum falcatum went isolates causing red rot of sugarcane in the northwest zone of India
 
More details
Hide details
1
Uttar Pradesh Council of Sugarcane Research Shahjahanpur-242001, Uttar Pradesh, India
CORRESPONDING AUTHOR
Ajay Kumar Tiwari
Uttar Pradesh Council of Sugarcane Research Shahjahanpur-242001, Uttar Pradesh, India
Submission date: 2012-08-30
Acceptance date: 2012-11-23
 
Journal of Plant Protection Research 2013;53(1):37–41
KEYWORDS
TOPICS
ABSTRACT
Red rot is one of the most wide spread sugarcane diseases in the country and has been a constraint on sugarcane productivity. Pathological as well as molecular studies were used to characterize the 11 isolates of Colletotrichum falcatum Went collected from sugarcane cultivars of different sugarcane-growing regions in northwestern states of India, to assess pathogen diversity. Seven reference pathotypes of C. falcatum from the northwestern zone of India were compared with four newly collected isolates of the same pathogen. All the newly collected isolates and existing pathotypes were inoculated on a set of 14 differentials in August 2011 by the plug method. After 60 days of inoculation, the observations were recorded and the pathotypes/isolates were categorized as resistant, intermediate, and susceptible according to the virulency behavior. On the basis of pathological categorization and comparison with reference pathotypes, it was concluded that the three isolates R1001 (CoJ 64), R1002 (CoS 88230), and R1004 (CoSe 92423) are similar to the existing isolate Cf 08, except for isolate R0401 from CoS 8436 (Shahjahanpur, Uttar Pradesh). This isolate differs from all the reference pathotypes of the northwestern zone of India indicating the existence of a new pathotype. Pathological results revealed that variety CoJ 64 is the ancestor/source of prevailing new races in nature because these three new isolates showed similarity with Cf 08, of CoJ 64. In this area, Cf 08 was widespread. The isolates were further tested for their variability with random amplification of polymorphic DNA (RAPD) primers. Twenty RAPD primers were screened, out of which seven gave amplification. Out of seven amplified primers, only two primers showed the polymorphism among 11 isolates (seven reference pathotypes and four new isolates) of C. falcatum. Analysis of the genetic coefficient matrix derived from the scores of RAPD profiles showed that minimum and maximum per cent similarities among the tested C. falcatum isolates existed in the range of 11.11 to 87.5, respectively. The dendogram analysis by the unweighted pair group method with arithmetic mean (UPGMA), separated two main clusters. The first cluster comprises only two isolates (Cf 07 & Cf 08), however the second cluster comprises all the other isolates (Cf 01, Cf 02, Cf 03, Cf 09, Cf 11, R1001, R1002, R1004 and R0401), confirming high genetic diversity among the isolates. The study also indicates the possibilities of a new isolate (R0401) in Shahjahanpur, which needs further investigation at the sequence level. The investigation is in progress.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (25)
1.
Abbas H., Anwar S.A., Javed N., Iqbal M.A., Abid N. 2010. Morphological varibality among isolates of C falcatum went. Infecting four cultivars of sugarcane. Pak. J. Phytopathol. 22 (2): 101–104.
 
2.
Agnihotri V.P. 1990. Diseases of Sugarcane and Sugarbeet. Oxford & IBH Pub. New Delhi, 283 pp.
 
3.
Alexander K.C., Viswanathan R. 1996. Major diseases affecting sugarcane production in India and recent experiences in quarantine. p. 46–48. In: “Sugarcane Germplasm Conservation and Exchange” (B.J. Croft, C.M. Piggin, E.S. Wallis, D.M. Hogarth, eds.). Canberra, Australia, Australian Centre for International Agricultural Research, 67 pp.
 
4.
Alvi A.K., Iqbal J., Shah A.H., Pan Y.B. 2008. DNA based genetic variation for red rot resistance in sugarcane. Pak. J. Bot. 40 (4): 1419–1425.
 
5.
Beniwal M.S., Satyavir, Virk K.S. 1989. Pathogenic variability in Colletotrichum falcatum incitant of red rot of sugarcane. Indian Phytopathol. 42 (3): 95–99.
 
6.
Chona B.L. 1954. Studies on the diseases of sugarcane. IV. Relative resistance of sugarcane varieties to red rot. Indian J. Agric. Sci. 24 (2): 301–315.
 
7.
Duttamajumdar S.K. 2008. Red Rot of Sugarcane. Lucknow India, Indian Institute of Sugarcane Research, p. 46.
 
8.
Freeman S. 1997. Pathogenicity and host specificity in the filamentous pathogen, Colletotrichum. p. 47–54. In: “Recent Advances in Microbiology” (B. Dagan). Israel, Department of Plant Path ARO, The Volcani Center.
 
9.
Hussnain Z., Afghan S. 2006. Impact of Major Cane Diseases on Sugarcane Yield and Sugar Recovery. Annual Report, Shakarganj Sugar Research Institute, Jhang, 43 pp.
 
10.
Jain S., Chahal S.S. 2011. Molecular and morpho-pathological variation in Bipolaris oryzae (Breda de Haan) shoem. causing brown spot of rice in North India. Crop Improvement 38 (2): 103–108.
 
11.
Karunanithi K., Ntthya K., Valluvaparidasan V., Paranidharan V., Velazhahan R., Jyaraj T. 2012. Genetic variability among the Isolates of Colletotrichum falcatum Went causing red rot of sugarcane in Tamil Nadu, India. International Society of Sugarcane Technologists, 10th Pathology Workshop, Nanning, China, May 7–11, 8 pp.
 
12.
Kumar N., Jhang T., Satyavir, Sharma T.R. 2010. Molecular and pathological characterization of Colletotrichum falcatum Infecting subtropical Indian sugarcane. J. Phytopathol. 154 (4): 260–267.
 
13.
Madan V.K, Bikas M., Ansari M.L., Srivastava A., Soni N., Solaman S., Agnihotri V.P. 2000. RAPD-PCR analysis of molecular variability in the red rot pathogen (Colletotrichum falcatum) of sugarcane. Sugarcane Int. 3 (2): 5–8.
 
14.
Mishra K., Behera B. 2009. Growth and sporulation of different isolates of Colletotrichum falcatum Went, incitant of sugarcane red rot in various culture media and hydrogen ion concentration. Indian Sugar 58 (10): 27–32.
 
15.
Mohan Raj D., Padmanabhan P., Karunakaran M. 2002 . Association of phytotoxin produced by Colletotrichum falcatum Went in the red rot disease of sugarcane. Sugarcane 5: 21–23.
 
16.
Muhammad W.A.K., Muhammad A., Raza W., Zia A. 2011. Identification of sugarcane lines with resistance to red rot. Pak. J. Phytopathol. 23 (2): 98–102.
 
17.
Saghai-Maroof M.A., Soliman K.M., Jorensen A.R., Allard R.W. 1984. Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA 81 (4): 8014–8018.
 
18.
Saiki R.K., Gelfand D.H., Stoffel S., Scharf S.J., Higushi R., Hom G.T., Mullis K.B., Erlich H.A. 1988. Primer directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239 (2): 487–489.
 
19.
Singh R.P., Lal S. 1999. Red rot. p. 153–158. In: “A Guide to Sugarcane Diseases” (P. Rott, R.A. Bailey, J.C. Comstock, B.J. Croft, A.S. Saumtally, eds.). CIRAD-ISSCT Publications, 339 pp.
 
20.
Singh D., Tiwari A.K., Mall S., Shukla B., Ahmad I.Z., Rao G.P. 2012. Morpholoical and molecular diversity among C. falcatum isolates causing red rot disease of sugarcane in Uttar Pradesh. 10th Pathology Workshop, Nanning, China, May 7–11, p. 9.
 
21.
Suman A., Lal S., Shasany A.K., Gaur A., Singh P. 2005. Molecular assessment of diversity among pathotypes of Colletotrichum falcatum prevalent in sub-tropical Indian sugarcane. World J. Microbiol. Biotech. 21 (6): 1135–1140.
 
22.
Tiwari A.K., Bharti Y.P., Mishra N., Tripathi S., Lal M., Sharma P.K., Rao G.P., Sharma M.L. 2010. Biotechnological Approaches for Improving Sugarcane Crop with Special Reference to Disease Resistance. Acta Phytopathol. Entomol. Hung. 45 (2): 235–249.
 
23.
Velazhahan R., Nithya K., Bukhari A.I.M., Valluvaparidasan V., Paraidharan V. 2012. Immonological and molecular detection of Colletotrichum falcatum went causing red rot disease in sugarcane (Saccharum Species Hybrids). International Society of Sugarcane Technologists, 10th Pathology Workshop, Nanning, China, May 7–11, 7 pp.
 
24.
Viswanathan R., Samiyappan R. 2002. Induced systemic resistance by fluorescent pseudomonas against red rot disease of sugarcane caused by Colletotrichum falcatum. Crop Prot. 21 (1): 1–10.
 
25.
Went FAFC. 1893. Het root snot. Arch. Java. Suikerind. 1: 265–282.
 
eISSN:1899-007X
ISSN:1427-4345