ORIGINAL ARTICLE
Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions
 
More details
Hide details
1
International Institute of Tropical Agriculture, Oyo Road, P.M. B. 5320, Ibadan, Nigeria
2
Department of Crop Protection, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria
CORRESPONDING AUTHOR
Oluwafemi Sunday
International Institute of Tropical Agriculture, Oyo Road, P.M. B. 5320, Ibadan, Nigeria
 
Journal of Plant Protection Research 2008;48(4):529–539
KEYWORDS
TOPICS
ABSTRACT
Field surveys were undertaken in 1997–1999 across five ecological zones in Nigeria to collect grass species showing symptoms of Maize streak virus (MSV) (Genus Mastrevirus: Family Geminiviridae). Apart from maize (Zea mays L.), 15 grass species were found with symptoms similar to MSV. These hosts showed two types of symptoms viz: mild or severe. Polymerase chain reaction (PCR) and immunocapture IC–PCR were used to diagnose the mastreviruse. Variation among the isolates is illustrated in relationship dendograms. The PCR dendogram showed 35–85% variation among the isolates while the IC–PCR dendogram showed 55–85%. The PCR dendogram clusters the isolates essentially along symptom severity. Isolates that were most distantly related to a standard MSV strain showed mild symptoms in their grass hosts. Simple PCR protocols could help in studying diversity among grass Mastreviruses where facilities for genome sequencing are not available.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (32)
1.
Bosque-Perez N.A. 2000. Eight decades of Maize streak virus research. Virus Res. 71: 107–121.
 
2.
Briddon R.W., Lunness P., Chamberlin L.C., Pinner M.S., Brundish H., Markham P.G. 1992. The nucleotide sequence of an infectious insect-transmissible clone of the geminivirus Panicum streak virus. J. Gen. Virol. 73: 1041– 1047.
 
3.
Briddon R.W., Lunness P., Chamberlin L.C., Markham P.G. 1994. Analysis of the genetic variability of Maize streak virus. Virus Genes 9: 93–100.
 
4.
Briddon R.W., Markham P.G. 1995. Use of PCR in the detection and characterization of geminiviruses. Bull. OEPP/EPPO Bull. 25: 315–320.
 
5.
Dellaporta S.L., Woods J., Hicks J.B. 1983. A plant DNA mini-preparation: version 11. Plant Biol. Rep. 1: 19–21.
 
6.
Damsteegt V.D. 1983. Maize streak virus 1. Host range and vunerability of maize germplasm. Plant Dis. 67: 734–737.
 
7.
Dekker E.L., Pinner M.S., Markham P.G., van Regenmontel M.H.V. 1988. Characterization of maize streak virus isolates from different plant species by polyclonal and monoclonal antibodies. J. Gen. Virol. 69: 983–990.
 
8.
Doyle J.J., Doyyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissues. Phytochem. Bull. 119: 11–15.
 
9.
Doyle J.J., Doyyle J.L. 1987. A rapid total DNA preparation procedure for fresh plant tissues. Focus 12: 13–15.
 
10.
Efron T., Kim S.K., Fajemisin J.M., Mareck J.H., Tang C.Y., Dąbrowski Z.T., Rossel H.W., Thottapplly G. 1989. Breeding for resistance to maize streak virus: a multidisciplinary team approach. Plant Breeding 103:1–36.
 
11.
Fauquet C.M., Bisaro D.M., Briddon R.W., Brown J.K., Harrison B.D., Rybicki E.P., Stenger D.C., Stanley J. 2003. Revision of taxonomic criteria for species demarcation in the family Geminiviridae, and an updated list of begomovirus species. Arch. Virol. 148: 405–421.
 
12.
Fajemisin J.M., Shoyinka S.A. 1976. Maize streak and other maize virus diseases in West Africa. p. 52–61. In: Proceedings International Maize Virus Disease Colloquium and Workshop. 16–19 August, 1976 Ohio Agric. Res. Dev. Centre Wooster, USA (L.E. Williams, D.T. Gordon, L.R. Nault, eds.).
 
13.
Hoffman K., Sackey S.T., Maiss E.., Adamako D., Vetten H.J. 1997. Immunocapture polymerase chain reaction for the detection and characterization of cacao swollen shoot virus 1A isolates. J. Phytopathol. 145: 205–212.
 
14.
Janson R.W., Siegel G., Lemon S.M. 1990. Molecular epidemiology of human hepatisis A virus defined by an antigen-captured polymerase chain reaction method. Proc. Natl. Acad. Sci. USA 87: 2867–2871.
 
15.
Martin D.P., Willment.J.A., Rybicki E.P. 1999) Evaluation of maize streak virus pathogenicity in differentially resistant Zea mays genotypes. Phytopathology 89: 695–700.
 
16.
Martin D.P., Willment J.A., Bilharz R., Velders R., Odhiambo B., Njuguna J., James D., Rybicki E.P. 2001. Sequence diversity and virulence in Zea mays of Maize streak virus isolates. Virology 288: 247–255.
 
17.
Mesfin T., Bosque-Perez N.A., Buddenhagen I.W., Thottappilly G. , Olojede S.O. 1992. Studies of maize streak virus isolates from grass and cereal hosts in Nigeria. Plant Dis. 76: 789–795.
 
18.
Naidu R.A., Hughes J.d’A. 2003. Methods for the detection of plant virus diseases. p 233–260. In: “Plant Virology in Sub-Saharan Africa’’ (J.d’A. Hughes, B.O. Odu, eds.). Proceedings of a conference organized by IITA, 4–8 June 2001, Ibadan, Nigeria.
 
19.
Oluwafemi S., Thottappilly G. , Alegbejo M.D. 2007. Transmission, ELISA, ans SDS–PAGE results of some maize streak virus isolates from different parts of Nigeria. J. Plant Protection Res. 47(2): 197–212.
 
20.
Okoruwa, A.E., 1995. Utilization and processing of maize. IITA Research Guide 35, 29 pp.
 
21.
Pinner M.S., Markham P.G. 1990. Serotyping and strain identification of maize streak virus isolates. J. Gen. Virol. 71: 1635–1640.
 
22.
Robertson N.L., French R., Gray S.M. 1991. Use of group specific primers and polymerase chain reaction for the detection and identification of luteoviruses. J. Gen. Virol. 72: 1473–1477.
 
23.
Rossell, H.W., Thottappilly G. 1985. Virus Diseases of Important Food Crops in Tropical Africa. IITA, Ibadan, Nigeria, 61 pp.
 
24.
Rohlf F.J. 1993. NTSYS–pc. Numerical taxonomy and multivariate analysis system. Exeter, New York.
 
25.
Rybicki E.P., Hughes F.L. 1990. Detection and typing of maize streak virus and other distantly related geminiviruses of grasses by polymerase chain reaction of a conserved viral sequence. J. Gen. Virol. 71: 2519–2526.
 
26.
Rybicki E.P., Dennis S., Napier G.Hughes F.L. 1998. Novel grass and wheat strains of maize streak virus detected by DNA amplification and sequencing. Mol. Plant Pathol. On-Line http//194.247.68.33/imppol/1988/0929rybycki.
 
27.
Rybicki E.P., Briddon R.W., Brown J.E. Fauquet C.M., Maxwell D.P., Harrison B.D. Markham P.G., Stanley J. 2000. Virus taxonomy. p. 285–297 In: “Seventh Report of the International Committee on Taxonomy of Viruses” (C.M. Fauquet, D.H.L. Bishop, E.B. Carsten, M.K. Estees, S.M. Lemmon, M.A. Mayo, D.J. McGeoch, C.R. Pringle, R.B. Wickner, eds.). Academic Press, San Diego.
 
28.
Schnippenkoetter W.H., Martin D., Hughes F., Fuvie M., Willment J., James D. von Wechmar B., Rybicki E.P. 2001. The relative infectivities and genomic characterization of three mastreviruses. Arch. Virol. 146: 1075–1088.
 
29.
Sneath P.H., Sokal R.R. 1973. Numerical taxonomy: The Principle and Practice of Numerical Classification. W.H. Freeman & Co. San Franscisco, USA, 573 pp.
 
30.
Soto P.E., Buddenhagen I.W., Asnani V.L. 1982. Development of streak virus-resistant maize populations through improved challenge and selection methods. Ann. Appl. Biol. 100: 539–546.
 
31.
Thottappilly G., Bosque-Perez N.A., Rossel H.W. 1993. Viruses and virus diseases of maize in tropical Africa. Plant Pathol. 42: 494–509.
 
32.
Willment J.A., Martin D.P., Rybicki E.P. 2001. Analysis of the diversity of African streak mastreviruses using PCR – generated PFLPs and partial sequence data. J. Virol. Methods 93: 75–87.
 
eISSN:1899-007X
ISSN:1427-4345