ORIGINAL ARTICLE
Search for variability among mastreviruses showing symptoms of maize streak virus from different regions of Nigeria by polymerase chain reactions
1
International Institute of Tropical Agriculture, Oyo Road, P.M. B. 5320, Ibadan, Nigeria
2
Department of Crop Protection, Ahmadu Bello University, P.M.B 1044, Zaria, Nigeria
Corresponding author
Oluwafemi Sunday
International Institute of Tropical Agriculture, Oyo Road, P.M. B. 5320, Ibadan, Nigeria
International Institute of Tropical Agriculture, Oyo Road, P.M. B. 5320, Ibadan, Nigeria
Journal of Plant Protection Research 2008;48(4):529-539
KEYWORDS
MastrevirusesMSVisolatespolymerase chain reaction (PCR)Immunocapture (IC)–PCRGraminaerelationship dendogramdiversity
TOPICS
ABSTRACT
Field surveys were undertaken in 1997–1999 across five ecological zones in Nigeria to collect grass species showing symptoms of Maize streak virus (MSV) (Genus Mastrevirus: Family Geminiviridae). Apart from maize (Zea mays L.), 15 grass species were found with symptoms similar to MSV.
These hosts showed two types of symptoms viz: mild or severe. Polymerase chain reaction (PCR) and immunocapture IC–PCR were used to diagnose the mastreviruse. Variation among the isolates is illustrated in relationship dendograms. The PCR dendogram showed 35–85% variation among the
isolates while the IC–PCR dendogram showed 55–85%. The PCR dendogram clusters the isolates essentially along symptom severity. Isolates that were most distantly related to a standard MSV strain showed mild symptoms in their grass hosts. Simple PCR protocols could help in studying diversity
among grass Mastreviruses where facilities for genome sequencing are not available.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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