ORIGINAL ARTICLE
Toxicity and physiological effect of essential oil of Artemisia annua (Labiatae) on Agriolimax agrestis L. (Stylommatophora: Limacidae)
 
More details
Hide details
1
Department of Plant Protection, College of Agriculture, University of Guilan, Rasht, 41335 Iran
CORRESPONDING AUTHOR
Jalal Jalali Sendi
Department of Plant Protection, College of Agriculture, University of Guilan, Rasht, 41335 Iran
 
Journal of Plant Protection Research 2012;52(2):185–189
KEYWORDS
TOPICS
ABSTRACT
Essential oil of Artemisia annua L. was investigated to find out its toxicity and physiological aspects on the slug Agriolimax agrestis, in controlled conditions (8±1°C, 75±5 RH and 14:10 LD). The slugs received different concentrations of essential oil treated radish leaves in methanol, while the control received methanol alone. LC 10 , LC 30 , LC 50 and LC 90 values were estimated at 4.67, 5.3, 5.81, 7.25%, respectively. The effect of the essential oil on some important enzymatic components like; cytochrome P450 monnooxygenase, acid phosphatase, alkaline phosphatase, lipase, amylase and protease were significantly increased compared to the control. These results indicate that the plant Artemisia annua L. not only shows toxicity but also shows some irreversible effect on some important biochemical components and deserves further investigation
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (23)
1.
Ascher K.R.S. 1993. Non-conventional insecticidal effects of pesticides available from the neem tree, Azadirachta indica. Arch. Insect. Biochem. Physiol. 22 (3–4): 433–449.
 
2.
Bernfeld P. 1955. Amylases, a and b. Methods Enzymol. 1: 149–158.
 
3.
Bessy O.A., Lowry O.H., Brock M.J. 1946. A method for the rapid determination of alkaline phosphate with five cubic millimeters of serum. J. Biol. Chem. 164 (1): 321–329.
 
4.
Bhakuni R.S., Jain D.C., Sharma R.P., Kumar S. 2001. Secondary metabolites of Artemisia Anna and their biological activity. Curr. Sci. 80 (1): 35–49.
 
5.
Cooper E.L. 1976. Comparative Immunology. Prentice-Hall, INC., Englewood Cliffs. New Jersy, USA, 338 pp.
 
6.
Elpidina E.N., Vinokurov K.S., Gromenko V.A., Rudenskaya Y.A., Dunaevsky Y.E., Zhuzhikov D.P. 2001. Compartmen talization of proteinases and amylases in Nauphoeta cinerea Midgut. Arch. Insect Biochem. Physiol. 48 (4): 206–216.
 
7.
Etebari K., Mirhoseini S.Z., Matindoost L. 2005. A study on intra specific biodiversity of eight groups of silkworm (Bombyx mori) by biochemical markers. Insect. Sci. 12 (2): 87–94.
 
8.
Farkas J. Farkas P., Hyde D. 2004. Liver and gastroenterology tests. p. 330–336. In: “Basic Skills in Interpreting Laboratory Data” (M. Lee, ed.). 3rd ed. Amer. Soc. Helth-Syst. Pharmacists, Bethesda, Maryland, USA, 618 pp.
 
9.
Glen D. M., Wiltshire C.W., Butler R.C. 1991. Slug population changes following molluscicide treatment in relation to distance from the edge of treated area. Crop Prot.10 (5): 408–412.
 
10.
Ham A.W., Gomak D.H. 1979. Histology. JB. Lippincott Company, Philadelphia and Toronto, 764 pp.
 
11.
Hansen L.G., Hodgson E. 1971. Biochemical characteristics of microsomes: N and Odemethylation. Biochem. Pharmac. 20 (7): 1569–1578.
 
12.
Isman M.B. 1999. Pesticides based on plant essential oils. Pesticide 10:68–72.
 
13.
Isman M.B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Ann. Rev. Entomol. 51: 45–66.
 
14.
Jalali J., Arbab A., Aliakbar A. 2005. The efficacy of Aqueous plant extracts of wormwood and dwarf elder against elm leaf beetle Xanthogaleruca luteola Mull. (Coleoptera: Chrysomelidae). Agri. Sci. Knowl. 15: 115–120.
 
15.
Jalali J., Haghigian F., Aliakber A. 2002. Comparative insecticidal efficacy of Artemisia Anna L. and Sambucus ebulus L. extracts on Tribolium confusum Duv. Iranian J. Agri. Sci. 34 (1): 313–319.
 
16.
Khanjani M. 2005. Vegetable Pests in Iran. Bu-Ali Sina University Publications, 719 pp.
 
17.
LeOra software. 1987. Polo-Pc: A User Guide to Probit or Logit Analysis. LeOra Software, Berkeley, California, 22 pp.
 
18.
Rao P.J., Maheshkumar K., Singh S., Subrah B.M. 1999. Effects of Artemisia annua oil on development and reproduction of Dysdercus koengii F. (Hem.: Pyrrhocoridae). J. Appl. Entomol. 123 (5): 315–318.
 
19.
SAS institute. 1997. SAS/STAT user’s guide for personal computers. SAS Institute, Cary, NC, 128 pp.
 
20.
Schmutterer H. 1990. Properties and potential of natural pesticides from the neem tree. Annu. Rev. Entomol. 35: 271–298.
 
21.
Schmutterer H. 1995. The Neem Tree Azadirachta India and Other Meliaceus Plants; Sources of Unique Natural Products for Integrated Pest Management, Medicine, Industry and Other Purposes. VCH publishers, Weinheim, Germany, 696 pp.
 
22.
Tsujita T., Ninomiya H., Okuda H. 1989. P-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue. J. Lipid. Res. 30 (7): 997–1004.
 
23.
Zibaee A., Bandani A.R., Ramzi S. 2008. Lipase and invertase activities in midgut and salivary glands of Chilo suppressalis(Walker Lepidoptera, Pyralidae), rice stem borer. Inv. Surv. J. 5: 180–189.
 
eISSN:1899-007X
ISSN:1427-4345