A study on the toxicity of a medicinal plant, Artemisia annua L. (Asteracea) extracts to the sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae)
More details
Hide details
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj 31584, Iran
Corresponding author
Zibaee Arash
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj 31584, Iran
Journal of Plant Protection Research 2010;50(1):79-85
A methanolic extract of Artemisia annua was obtained to evaluate its insecticidal activities against the sunn pest (Eurygaster intefriceps). Also, the responses of general esterase (EST), glutathione S-transferase (GST), alkaline phosphatase (ALP), acid phosphatase (ACP), acethylcholinesterase (AChE) to the plant extract were investigated. Topical application of plant extract on adults showed that the mortality was dose-dependent i.e. with increasing of plant extract concentrations more mortality achieved. Esterase and GST activities were increased in the first 24 h post-treatment. However, the enzymes activities were decreased after 24 h until 72 h. The activities of ALP, ACP and AChE in insect body decreased significantly and inhibition was higher along with increasing concentrations of plant extract. Isozyme electrophoresis profiles indicated that responses of isozymes (EST and GST) to plant extract were decreased after 48 h exposure to extract so that some enzymes bands disappeared. The results indicated that the highest concentration of A. annua extract was the most toxic among the four extracts. The decline of the detoxification ability in insects’ tissues might be the main reason for the insecticidal activities.
The authors have declared that no conflict of interests exist.
Akhtar Y., Isman M.B. 2004. Comparative growth of inhibitory and antifeedant effects of plant extract and pure allelochemicals on some phytophagous insect species. J. Appl. Entomol. 128: 32–38.
Bessey O.A., Lowry O.H., Brock, M.J. 1946. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J. Biol. Chem. 164: 321–329.
Bhakuni R.S., Sharma R.P., Kumar S. 2001. Secondary metabolites of Artemisia annua and their biological activity. Curr. Sci. 80: 35–49.
Bradford M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Chapman R.F. 1985. Structure of the digestive systems. p. 165–211. In: “Comprehensive Insect Physiology, Biochemistry, and Pharmacology” Vol. 14 (G.A. Kerkut, L.I. Gilbert, eds.). Pergamon Press, Oxford.
Francis F., Haubruge E., Gasper C. 2000. Influence of host plants on specialist/generalists aphids on the development of Adalis bipunctata (Coleoptera: Coccinellidae). Eur. J. Entomol. 97: 481–490.
Grant D.F., Matsumura F. 1989. Glutathione S-transferase 1 and 2 in susceptible and insecticide resistant Aedes aegypti. Pestic. Biochem. Physiol. 33: 132–138.
Grundy D.L., Still C.C. 1985. Inhibition of acetylcholinesterases by pulegone-1,2-epoxide. Pestic. Biochem. Physiol. 23: 383–388.
Han Q.P., Zhuang Z., Tang A. 1995. The mechanism of resistance to fenitrothion in Chilo suppressalis Walker. Acta Entomol. Sin. 38: 266–272.
Hemingway J., Karunatne S.H.P.P. 1998. Mosquito carboxylesterases: A review of the molecular biology and biochemistry of a major insecticide resistance mechanism. Med. Veter. Entomol. 12: 1–12.
Isman M.B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Ann. Rev. Entomol. 51: 45–66.
Jalali J., Arbab A., Aliakbar A. 2005. The efficacy of Aqueous plant extracts of wormwood and dwarf elder against elm leaf beetle Xanthogaleruca luteola Mull. (Coleoptera: Chrysomelidae). Agri. Sci. 15: 115–120.
Kazzazi M., Bandani A.R., Hoszeinkhani S. 2005. Biochemical characterization of α-amylase of the Sunn pest, Eurygaster integriceps. Entomol. Sci. 8: 371–377.
Ke W., Yang J., Meng Z., Ma A. 2008. Evaluation of molluscicidal activities of Arisaema tubers extracts on the snail Oncomelania hupensis. Pestic. Biochem. Physiol. 92: 129–132.
Korayem A.M., Hasabo S.A., Ameen H.H. 1993. Effects and mode of action of some plant extracts on certain plant parasitic nematodes. Anz. Schaedlingsku. 66: 32–36.
LeOra Software 1987. Polo-Pc a User’s Guide to Probit or Logit Analysis. LeOra Software, Berkeley, California, 22 pp.
Li F., Han Z. 2002. Purification and characterization of acetylcholinesterase from cotton aphid, Aphis gossypii. Arch. Insect Biochem. Physiol. 51: 37–45.
Morris J.G. 1978. A Biologist’s Physical Chemistry (2nd ed.). Edward Arnold Publishing (Limited).
Nath B.S., Suresh A., Mahendra Varma B., Kumar R.P. 1997. Changes in protein metabolism in haemolymph and fat body of the silkworm, Bombyx mori L., in response to organophosphorus insecticides toxicity. Ecotoxicol. Environ. Saf. 36: 169–173.
Oehmichen M., Besserer K. 1982. Forensic significance of acetylcholine esterase histochemistry in organophosphate intoxication. Original investigations and review of the literature. Z. Rechtsmed. 89: 149–165.
Oppenorth F.J. 1979. Glutathione S-transferase and hydrolytic activity in a tetrachlorvinphos-resistant strain of housefly and their influence on resistance. Pestic. Biochem. Physiol. 11: 176–178.
Robertson J.L., Preisler H.K., Russell R.M. 2007. PoloPlus: Probit and Logit Analysis User’s Guide. LeOra Software, Petaluna, CA, USA, 245 pp.
Sakharov I.Y., Makarova I.E., Ermolin G.A. 1989. Chemical modification and composition of tetrameric isozyme K of alkaline phosphatase from harp seal intestinal mucosa, Comp. Biochem. Physiol. Part B. 92: 119–122.
SAS Institute. 1997. SAS/STAT User’s Guide for Personal Computers. SAS Institute, Cary, Nc.
Senthil Nathan S. 2006. Effects of Melia azedarach on nutritional physiology and enzyme activities of the rice leaffolder Cnaphalocrocis medinalis (Gnenee) (Lepidoptera: Pyralidae). Pestic. Biochem. Physiol. 84: 98–108.
Senthil Nathan S., Kalaivani K. 2005. Efficacy of nucleopolyhedrovirus (NPV) and azadirachtin on Spodoptera litura Fabricius (Lepidoptera: Noctuidae). Biol. Control 34: 93–98.
Senthil Nathan S., Choia M.Y., Seoa H.Y., Paika C.H., Kalaivania K., Kim J.D. 2008. Effect of azadirachtin on acetylcholinesterase (AChE) activity and histology of the brown planthopper Nilaparvata lugens. Ecotoxicol. Environ. Saf. 70: 244–250.
Shafeek A., Prasanthi R.P.J., Reddy G.H., Chetty C.S., Reddy G.R. 2004. Alterations in acetylcholinesterase and electrical activity in the nervous system of cockroach exposed to the neem derivative, azadirachtin. Ecotoxicol. Environ. Saf. 59: 205–208.
Shekari M., Jalali Sendi J., Etebari K., Zibaee A., Shadparvar A. 2008. Effects of Artemisia annua L. (Asteracea) on nutritional physiology and enzyme activities of elm leaf beetle, Xanthogaleruca luteola Mull. (Coleoptera: Chrysomellidae). Pestic. Biochem. Physiol. 91: 66–74.
Singh K., Singh D.K. 2000. Toxicity to the snail Limnaea acuminata of plant-derived molluscicides in combination with synergists. Pest Manage. Sci. 56: 889–898.
Stryer L. 1995. Biochemistry. W.H. Freeman and Company, New York, 415 pp.
Tripathi A.K.V., Prajapati A.K., Aggarwal-Khanuja S.P.S., Kumar S. 2000. Repellency and toxicity of oil from Artemisia annua to certain stored-product beetles. J. Econ. Entomol. 93: 43–47.
Vanhaelen N., Haubruge E., Lognay G., Francis F. 2001. Hoverfly Glutathione S-Transferases and Effect of Brassicaceae Secondary Metabolites. Pestic. Biochem. Physiol. 71: 170–177.
Wang J.J., Cheng W.X., Ding W., Zhao Z.M. 2004. The effect of the insecticide dichlorvos on esterase activity extracted from the psocids, Liposcelis bostrychophila and L. entomophila. J. Insect Sci. 4: 1–5.
Weinzierl R., Henn T. 1991. Alternatives in insect management: biological and biorational approaches. North Central Regional Extension Publication 401. Cooperative Extension Service, University of Illinois at Urbana-Champaign, 73 pp.
Wilson K. 1986. Principle and Techniques of Practical Biochemistry (K. Wilson, K.H. Goulding, eds.). 3rd ed. Edward Arnold, 229 pp.
Yang M.L., Wu H.H., Guo Y.P., Ma E.B. 2005. Toxicological and biochemical characterizations of malathion sensitivity in two populations of Oxya chinensis (Orthoptera: Acrididae). Insect Sci. 13: 41–47.
Yu S.J. 1982. Host plant induction of glutathione S-transferase in the fall armyworm. Pestic. Biochem. Physiol. 17: 52–60.
Journals System - logo
Scroll to top