ORIGINAL ARTICLE
Biological control potential of Trichoderma asperellum against Pseudocercospora griseola, the etiological agent of angular leaf spot in common bean (Phaseolus vulgaris L.)
1
Crop Protection, Institute of Agricultural Research for Development (IRAD), Yaoundé, Cameroon
2
Department of Biochemistry, University of Yaoundé, Yaoundé, Cameroon
3
Post-Harvest Unit, Ekona Agricultural Research Centre, Institute of Agricultural Research for Development (IRAD), Buea, Cameroon
4
Department of Agriculture, University of Dschang, Dschang, Cameroon
5
Annual Crop, International Institute for Tropical Agriculture (IITA), Kinshasa, Congo
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2025-07-16
Acceptance date: 2025-09-23
Online publication date: 2026-05-06
Corresponding author
Gael Chatue Chatue
Crop Protection, Institute of Agricultural Research for Development (IRAD), Yaoundé, Cameroon
Crop Protection, Institute of Agricultural Research for Development (IRAD), Yaoundé, Cameroon
HIGHLIGHTS
- T. asperellum PR11, PR12, and 659-7 completely inhibited the growth of P. griseola
- T. asperellum PR11 protects bean leaves from angular leaf spot disease
- Protection of bean was associated with an increase in total phenols and flavonoids
KEYWORDS
TOPICS
ABSTRACT
Angular leaf spot disease, which is caused by the fungus Pseudocercospora griseola, is
among the most damaging diseases affecting common bean (Phaseolus vulgaris L.), impacting
both yield and grain quality. Because of the environmental risks associated with
fungicides and the variability in the virulence of P. griseola isolates, biological control
emerges as a promising alternative for managing this disease. This study assessed the biological
control potential of Trichoderma asperellum 659-7, PR11, and PR12 against P. griseola.
Additionally, changes in some biochemical parameters were also investigated. The
findings revealed that the three tested strains stopped the growth of P. griseola during the
confrontation test, achieving 100% inhibition. Furthermore, the cell-free culture filtrates
from each T. asperellum strain hindered the mycelial growth and spore germination of
P. griseola, with the level of inhibition depending on both the concentration of culture filtrate
and the specific strain of T. asperellum. The most significant reduction was noted with
T. asperellum PR11, which decreased mycelial growth by 26.33% and spore germination by
27.14% at 25% (v/v). Moreover, treating infected bean leaves with T. asperellum PR11 led
to a reduction in disease severity by 11.32 and 22.5% at 14 and 21 days after inoculation,
respectively. An increase in chlorophyll content (287.087%), total phenols (43.116%), and
flavonoids (72.010%) was also observed when infected leaves were treated with T. asperellum
PR11. These overall results endorse the effectiveness of Trichoderma asperellum PR11
as a biological control agent for managing bean angular leaf spot, offering an alternative
and environmentally friendly strategy.
ACKNOWLEDGEMENTS
This research was carried out in the Laboratory of
Biological Control and Applied Microbiology of the
Institute of Agricultural Research for Development
(IRAD). Therefore, our sincere gratitude is extended to
IRAD for providing access to their laboratory facilities.
We also thank Bertrand Zing Zing and Etienne Akoutou
for their assistance and support.
RESPONSIBLE EDITOR
Piotr Iwaniuk
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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