Characterization of angular leaf spot (ALS) disease of beans caused by Pseudocercospora griseola (Sacc.) Crous & Braun along with its occurrence was investigated using 118 isolates obtained from beans grown in greenhouses in the western Black Sea region of Turkey. Incidences of ALS disease ranged between 77–100% and 82–100% for summer and autumn sown bean cultivations while the disease severity was in the ranges of 66–82% and 74–86% for the same periods, respectively. All of the 118 isolates of P. griseola yielded 500–560 bp PCR products from ITS1 and ITS4 primers, while 45 isolates yielded 200–250 bp products from actin genes primer and 5 isolates yielded 300–350 bp from calmodulin primer. The form of the Turkish isolates of P. griseola was determined as f. griseola since ITS sequences of 118 isolates of P. griseola showed between 98–100% similarity to the isolates of P. griseola f. griseola deposited in GenBank and our isolates took place on the same branch on the phylogenetic tree formed by the representative isolates in GenBank. The actin sequences did not give a clear differentiation for the forms of P. griseola. The phylogenetic trees generated by ITS1, ITS2 and actin genes formed similar branches. Each had two main clade and similar sub clades.
This study is the Ph.D. thesis of Ankara University Graduate School of Natural and Applied Sciences Department of Plant Protection named ”Determination of the Groups of Pseudocercospora griseola Causing Angular Leaf Spot in Protected Beans in West Black Sea Region, Investigations on Prevalence, Searches of Inoculum and Reactions of Some Bean Cultivation Against This Agent’’ and this project was supported by the Turkish Ministry of Agriculture and Forestry with Project number TAGEM-BS-/10/10-01/02-05 under National Integrated Greenhouse Disease Management Program.
The authors have declared that no conflict of interests exist.
Abadio A.K.R., Lima S.S., Santana M.F., Salamao T.M.F., Sartorato A., Mizubuti E.S.G., Araujo E.F., Queiroz de M.V. 2012. Genetic diversity analysis of isolates of the fungal bean pathogen Pseudocercospora griseola from central and southern Brazil. Genetics and Molecular Research 11 (2): 1272–1279. DOI: 10.4238/2012.May.14.1.
Bora T., Karaca İ. 1970. Kültür Bitkilerinde Hastalığın ve Zararın Olçülmesi. [Measurement of Disease and Damage in Cultivated Plants]. Ege University, Faculty of Agriculture Auxiliary Textbook, No. 167. (in Turkish).
Canpolat S., Maden S. 2017. Determination of the inoculum sources of angular leaf spot disease caused by Pseudocercospora griseola, on common beans. Plant Protection Bulletin 57 (1): 39–47 (in Turkish, with English abstract). DOI: 10.16955/bitkorb.299016, ISSN 0406-3597.
Canpolat S., Maden S. 2020. Reactions of some common bean cultivars grown in Turkey against some isolates of angular leaf spot disease, caused by Pseudocercospora griseola. Plant Protection Bulletin 60 (2): 45–54. (in Turkish, with English abstract). DOI: 10.16955/bitkorb.630968.
Chilagane L.A., Nchimbi-Msolla S., Kusolwa P.M., Porch T.G., Diaz L.M.S., Tryphone G.M. 2016. Characterization of the common bean host and Pseudocercospora griseola, the causative agent of angular leaf spot disease in Tanzania. African Journal of Plant Science 10 (11): 238–245. DOI: https://doi.org/10.5897/AJPS20....
Crous P.W., Lienbenberg M.M., Braun U., Groenewald J.Z. 2006. Re-evaluating the taxonomic status of Phaeoisariopsis griseola, the causal agent of angular leaf spot of bean. Studies in Mycology 55 (1): 163–173. DOI: 10.3114/sim.55.1.163.
Ddamulira G., Mukankusi C.M., Ochwo-Ssemakula M., Edema R., Sseruwagi P., Gepts P.L. 2014. Distribution and variability of Pseudocercospora griseola in Uganda. Journal of Agricultural Science 6 (6): 16–29. DOI: 10.5539/jas.v6n6p16.
Nay M.M., Souza T.L.P.O., Gonçalves-Vidigal M.C., Raatz B., Mukankusi C.M., Gonçalves-Vidigal M.C., Abreu A.F.B., Melo L.C., Pastor-Corrales M.A. 2019. A review of angular leaf spot resistance in common bean. Crop Science 59: 1376–1391. DOI: 10.2135/cropsci2018.09.0596.
Sartorato A. 2004. Pathogenic variability and genetic diversity of Phaeoisariopsis griseola isolates from two counties in the State of Goias, Brazil. Journal of Phytopathology 152: 385–390.
Schoonhoven A., Pastor-Corrales M.A. 1987. Standard system for the evaluation of bean germplasm. Centro Internacional de Agricultura Tropical, CIAT Apartado Areo 6713 Cali, Colombia, p. 56.
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA 6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30 (12): 2725.
Townsend G.K., Heuberger J.W. 1943. Methods for estimating losses caused by diseases in fungicide experiments. Plant Disease Report 27: 340–343.
Viguiliouk E., Mejia S.B., Kendall C.W., Sievenpiper J.L. 2017. Can pulses play a role in improving cardiometabolic health. Evidence from systematic reviews and meta-analyses. Annuals of the New York Academy of Sciences 1392 (1): 43.
Journals System - logo
Scroll to top