ORIGINAL ARTICLE
Detection and genetic variability of newly identified dasheen mosaic virus in Poland
1
Department of Virology and Bacteriology, Institute of Plant Protection – National Research Institute, Poznań, Poland
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-04-26
Acceptance date: 2022-08-08
Online publication date: 2022-10-10
Corresponding author
Beata Hasiów-Jaroszewska
Department of Virology and Bacteriology, Institute of Plant Protection–National Research Institute, Węgorka 20, 60-318, Poznań, Poland
Department of Virology and Bacteriology, Institute of Plant Protection–National Research Institute, Węgorka 20, 60-318, Poznań, Poland
Journal of Plant Protection Research 2022;62(4):307-316
HIGHLIGHTS
- This is the first report of DsMV in Poland.
- DsMV population is highly diverse.
- Variability of DsMV was confirmed by low sequence identity and recombination events.
- On phylogenetic tree the Polish isolates grouped separately from others DsMV isolates.
KEYWORDS
TOPICS
ABSTRACT
Dasheen mosaic virus (DsMV) is one of the most important viral pathogens of aroids and
can cause major economic losses for ornamental crops. Here, we present the detection and
molecular characterisation of DsMV isolates originating from Monstera adansonii plants
in Poland. Moreover, the genetic variability of DsMV isolates was analyzed based on the
coat protein gene (CP) of the Polish and other DsMV isolates described to date. The pre-
sence of DsMV was confirmed by transmission electron microscopy (TEM) and reverse
transcription polymerase chain reaction (RT-PCR) with specific, diagnostic primers in
three out of ten examined plants. To obtain full-length sequences of CP, two pairs of pri-
mers were designed and used in the RT-PCR. The specificity of obtained products was con-
firmed by Sanger sequencing. The obtained sequences of CP were compared with 44 other
DsMV sequences retrieved from the GenBank. Analyses revealed that DsMV population
is very diverse. The variability of DsMV isolates was confirmed by low sequence identity
and pervasive recombination events. The phylogenetic analysis was performed based on
37 non-recombinant CP sequences. The maximum-likelihood reconstruction revealed
that the Polish isolates are distinct and grouped separately from other DsMV isolates. Due
to the high genetic diversity, detecting the virus could be difficult. Nonetheless disease
management relies strongly on a fast and accurate identification of the causal agent. To our
knowledge this is the first report of DsMV in Poland.
FUNDING
This study was supported by the Polish Ministry of
Education and Science, by Statutory activity project:
WIB03.
RESPONSIBLE EDITOR
Yongzhi Wang
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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