Effect of amino acid application on induced resistance against citrus canker disease in lime plants
More details
Hide details
Department of Plant Protection, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran
Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14155-6343, Tehran, Iran
Department of Biotechnology, Faculty of New Technologies and Energy Engineering, Shahid Beheshti University G.C., 1194968319, Evin, Tehran, Iran
Submission date: 2013-12-11
Acceptance date: 2014-05-07
Corresponding author
Vahideh Hasabi
Department of Plant Protection, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran
Journal of Plant Protection Research 2014;54(2):144-149
Citrus bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc), is a destructive disease. So far, the chemicals used to control this pathogen are either ineffective or harmful to the environment. To improve control of this disease, lime (Citrus aurantifolia) were treated with L-arginine, L-methionine, L-ornithine, and distilled water. Plants were inoculated with Xcc, 48 hours post treatment. Lesion diameters of inoculated leaves were evaluated four weeks after inoculation with a bacterial suspension. Changes in β-1,3-glucanase transcript levels and activity of antioxidant enzymes, catalase, peroxidase, and phenylalanine ammonia-lyase were investigated at 48 hours post treatment and 24, 48, and 72 hours post inoculation. Based on the results of phenotypic, antioxidant enzyme activity and a molecular study of the stressed plants, it was found that those plants treated with the amino acid methionine significantly increased the plant induced resistance as well as decreased the severity of disease by reducing necrotic lesion size.
The authors have declared that no conflict of interests exist.
Ballester A.R., Izquierdo A., Lafuente M.T., González-Candelas L. 2010. Biochemical and molecular characterization of induced resistance against Penicillium digitatum in citrus fruit. Postharvest Biol. Technol. 56 (1): 31–38.
Bradford M.M. 1976. A rapid and sensitive method for quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1): 248–254.
Cohen Y.R. 2002. β-Amino-butryric acid-induced resistance against plant pathogens. Plant Dis. 86 (5): 448–457.
Cohen Y., Niderman T., Mosinger E., Fluhr R. 1994. β-Aminobutyric acid induces the accumulation of pathogenesis-related proteins in tomato (Lycopersicon esculentum Mill.) and resistance to late blight infections caused by Phytophthora infestans. Plant Physiol. 104 (1): 59–66.
Dekkers M.G.H., Graham J.H., Burns J.K., Cubero J., Colburn G.C. 2004. Evaluation of chemical inducers and PR protein reporters for induced systemic resistance to citrus bacterial diseases. Phytopathology 94 (6): S25.
Dewdney M.M., Graham J.H. 2012. Florida Citrus Pest Management Guide: Citrus Canker. Institute of Food and Agricultural Sciences, University of Florida, 4 pp.
Dhindsa R.S., Dhindsa P., Thorpe A.T. 1981. Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decrease levels of superoxide dismutase and catalase. J. Exp. Bot. 32 (1): 93–101.
Dixon R.A., Paiva N.L. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7 (7): 1085–1097.
Droby S., Wisniewski M., Macarisin D., Wilson C. 2009. Twenty years of posthar-vest biocontrol research: is it time for a new paradigm? Postharvest Biol. Technol. 52 (2): 137–145.
Francis M.I., Redondo A., Burns J.K., Graham J.H. 2009. Soil application of imidacloprid and related SAR-inducing compounds produces effective and persistent control of citrus canker. Eur. J. Plant Pathol. 124 (2): 283–292.
Gabriel D.W., Kingsley M.T., Hunter J.E., Gottwald T.R. 1989. Reinstatement of Xanthomonas citri (ex Hasse) and X. phaseoli (ex Smith) and reclassification of all X. campestris pv. citri strains. Int. J. Syst. Bacteriol. 39 (1): 14–22.
Gapinska M., Sklodowska M., Gabara B. 2008. Effect of short- and long-term salinity on the activities of antioxidative enzymes and lipid peroxidation in tomato roots. Acta Physiol. Plant 30 (1): 11–18.
Graham J.H., Myers M.E. 2011. Soil application of SAR inducers imidacloprid, thiamethoxam, and acibenzolar-S-methyl for citrus canker control in young grapefruit trees. Plant Dis. 95 (6): 725–728.
Graham J.H., Leite R.P., Jr. 2007. Soil applied neonicotinoids for control of bacterial diseases on young citrus trees. p. 107. In: Proc. of International Workshops: “PR-Proteins” and “Induced Resistance Against Insects and Diseases” Doorn, The Netherlands, 10–14 May 2007.
Hammerschmidt R., Métraux J.-P., van Loon L.C. 2001. Inducing resistance: a summary of papers presented at the First International Symposium on Induced Resistance to Plant Diseases, Corfu, May 2000. Eur. J. Plant Pathol. 107 (1): 1–6.
Jakab G., Cottier V., Toquin V., Rigoli G., Zimmerli L. 2001. β-Aminobutyric acidinduced resistance in plants. Eur. J. Plant Pathol. 107 (1): 29–37.
Li J., Zingen-Sell I., Buchenauer H. 1996. Induction of resistanceof cotton plants to Verticillium and of tomato plants to Fusarium wilt by 3-aminobutyric acid and methyl jasmonate. J. Plant Dis. Prot. 103 (3): 288–299.
Ojha S., Chandra Chatterjee N. 2012. Induction of resistance in tomato plants against Fusarium oxysporum f. sp. lycopersici mediated through salicylic acid and Trichoderma harzianum. J. Plant Prot. Res. 52 (2): 220–225.
Oka Y., Cohen Y., Spiegel Y. 1999. Local and systemic induced resistance to the root-knot nematode in tomato by DL-β-amino-n-butyric acid. Phytopathology 89 (12): 1138–1143.
Reimers P.J., Leach J.E. 1991. Race-specific resistance to Xanthomonas oryzae pv. oryzae conferred by bacterial blight resistance gene Xa-10 in rice (Oryza sativa) involves accumulation of a lignin-like substance in host tissues. Physiol. Mol. Plant Pathol. 38 (1): 39–55.
Reuveni R. 1995. Biochemical marker of disease resistance. p. 99–114. In: “Molecular Methods in Plant Pathology” (R.P. Singh, U.S. Singh, eds.). Boca Raton, CRC Press, Florida, USA, 507 pp.
Sarosh B.R., Sivaramakrishnan S., Shetty H.S. 2005. Elicitation of defense related enzymes and resistance by L-methionine in pearl millet against downy mildew disease caused by Sclerospora graminicola. Plant Physiol. Biochem. 43 (8): 808–815.
Tzeng D.D., Tzeng H.C., Chen R., Cheng A., Tsai C.C., Chen C., Hwang T., Yeh Y., DeVay J.E. 1996. The use of MR formulation as a novel and environmentally safe photodynamic fungicide for the control of powdery mildews. Crop Prot. 15 (4): 341–347.
Van Loon L.C., Rep M., Pieterse C.M.J. 2006. Significance of inducible defense-related proteins in infected plants. Annu. Rev. Phytopathol. 44 (1): 135–162.
Yao H.J., Tian S.P. 2005. Effects of a biocontrol agent and methyl jasmonate on postharvest diseases of peach fruit and the possible mechanisms involved. J. Appl. Microbiol. 98 (4): 941–950.
Zimmerli L., Jakab G., Matraux J.P., Mauch-Mani B. 2000. Potentiation of pathogen-specific defense mechanisms in Arabidopsis by β-aminobutyric acid. Proc. Natl. Acad. Sci. USA 97 (23): 12920–12925.
Journals System - logo
Scroll to top