Field application of Clethodim herbicide combined with Trichoderma spp. for controlling weeds, root knot nematodes and Rhizoctonia root rot disease in two faba bean cultivars
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Botany Department, Weed Biology and Control, National Research Centre, Dokki, Cairo, Egypt
Plant Pathology Department, National Research Centre, Dokki, Cairo, Egypt
Nematology Laboratory, Plant Pathology Department, National Research Centre, Dokki, Cairo, Egypt
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-10-01
Acceptance date: 2019-06-26
Online publication date: 2019-07-18
Corresponding author
Mahmoud A. T. El-Dabaa   

Botany Department, Weed Biology and Control, National Research Centre, Dokki, Cairo, Egypt
Journal of Plant Protection Research 2019;59(2):255-264
Clethodim herbicide (Cle) and three Trichoderma strains (Tri) were applied either alone or in combination (Cle + Tri) for controlling weeds, root knot nematodes (Meloidogyne arenaria) and Rhizoctonia root rot disease (Rhizoctonia solani) as well as for evaluating their effects on total microbial count in the rhizosphere and the number of Rhizobium nodules on roots in two faba bean cultivars cultivated in naturally heavily infested fields. The evaluated characters were very similar for the two tested cultivars (Nubariya 1 and Sakha 3). Treatment with Cle alone highly reduced the fresh and dry matter of tested weeds (Amaranthus viridis, Cynodon dactylon and Cenchrus ciliaris), followed by Cle + Tri and Tri alone. Cle + Tri highly reduced nematode parameters viz. numbers of J2 in soil or roots, females, eggs, galls and egg-masses when compared with each treatment alone. Tri alone caused a great decrease in Rhizoctonia root rot infection, followed by Cle + Tri and Cle alone. Total microbial count and Rhizobium nodules were affected only with Cle treatment. Plant growth parameters (shoot length, shoot fresh and dry weight and numbers of branches and leaves) and yield parameters (fresh pod and dry weight, seed number per pod, seed weight and ash pod weight of plant) were greatly improved for Cle + Tri treatments when compared with either Tri or Cle alone.
The authors have declared that no conflict of interests exist.
Abd El-Hai K.M., Ali Abeer A., El-Metwally M.A. 2017. Down-regulation of damping-off and root rot diseases in lentil using kinetin and Trichoderma. International Journal of Agricultural Research 12: 41−51. DOI: 10.3923/ijar.2017.41.51.
Aboali Z., Saeedipour S. 2015. Efficacy evaluation of some herbicides for weed management and yield attributes in broad bean (Vicia faba). Research Journal of Environmental Sciences 9: 289−295. DOI: 10.3923/rjes.2015.289.295.
Arevalo G.R.C., Lusarreta C.A., Neyra C.B., Sanchez M.A., Algarra P.J.H. 1992. Chemical control of annual weeds in field beans (Vicia faba) in Central Spain. Weed Science 40 (1): 96−100.
Barker K.R. 1985. Nematode extraction and bioassays. p. 19−35. In: “An Advanced Treatise on Meloidogyne.” Vol. II. (K.R. Barker, C.C. Carter, J.N. Sasser, eds.). North Carolina State University Graphics, Raleigh, USA.
Barnett H.L., Hunter B.B. 1972. Illustrated Genera of Imperfect Fungi. Burgess Publ. Co., Minnesota, 241 pp.
Bridson E.Y. 1995. The Oxide Manual. 7th ed. Unipath Limited, Wade Koad, Basingstoke Hampshire, RG 248 PW, England.
El-Mougy N.S., Abdel-Kader M.M. 2009. Seed and soil treatments as integrated control measure against faba bean root rot pathogens. Plant Pathology Bulletin 18: 75−87.
El-Nagdi Wafaa M.A., Youssef M.M.A. 2004. Soaking faba bean seed in some bio-agents as prophylactic treatment for controlling Meloidogyne incognita root-knot nematode infection. Journal of Pest Science 77 (2): 75–78. DOI:10.1007/s10340-003-0029-y.
El-Nagdi Wafaa M.A., Abd-El-Khairb H. 2014. Biological control of Meloidogyne incognita and Fusarium solani in dry common bean in the field. Archives of Phytopathology and Plant Protection 47 (4): 388–397. DOI: 10.1080/03235408.2013.809931.
Elshahawy I.E., Haggag Karima H.E., Abd- El-Khair H. 2016. Compatibility of Trichoderma spp. with seven chemical fungicides used in the control of soil borne plant pathogens. Research Journal of Pharmaceutical, Biological and Chemical Sciences 7 (1): 1772−1785.
El-Shennawy Rania Z. 2011. Biological control of root-rot and wilt disease of faba bean using some bioagents. Journal of Plant Protection and Pathology, Mansoura University 2 (2): 195–202.
Farfour S.A., Al-Saman M.A. 2014. Root-rot and stem-canker control in Faba bean plants by using some biofertilizers agents. Journal of Plant Pathology and Microbiology 5: 218. DOI: 10.4172/2157-471.1000218.
Gampala K., Pinnamaneni R.R. 2010. Studies on the compatibility of Trichoderma viride with certain agro-chemicals. Current World Environment 5 (1): 155−158. DOI: http://dx.doi.org/10.12944/CWE....
Habtegebriel B., Boydom A. 2016. Biocontrol of faba bean black root rot caused by Fusarium solani using seed dressing and soil application of Trichoderma harzianum. Journal of Biological Control 30 (3): 169−176. DOI: https://doi.org/10.18311/jbc/2....
Jordan D.L. 2011. Weed management in peanuts. p. 56–62. In: “2011 Peanut Information”. North Carolina Cooperation Extension Service Publication AG-331.
Khan M.R., Ashraf S., Rasool F., Salati K.M., Mohiddin F.A., Haque Z.Z. 2014. Field performance of Trichoderma species against wilt disease complex of chickpea caused by Fusarium oxysporum f. sp. ciceri and Rhizoctonia solani. Turkish Journal of Agriculture and Forestry 38: 447−454. DOI:10.3906/TAR-1209-10.
Kleemann S.G.L., Gill G.S. 2012. Herbicide application strategies for the control of rigid ryegrass (Lolium rigidum) in widerow Faba bean (Vicia faba) in Southern Australia. Weed Technology 26: 284–288. DOI: https://doi.org/10.1614/WT-D-1....
Korayem A.M., Mohamed M.M.M., El-Ashry S.M. 2018. Estimation of yield losses in broad bean due to Meloidogyne arenaria in Egypt. Pakistan Journal of Nematology 36 (1): 33−40. DOI: http://dx.doi.org/10.18681/pjn....
Madhavi G.B., Bhattiprolu S.L., Reddy V.B. 2011. Compatibility of biocontrol agent Trichoderma viride with various pesticides. Journal of Horticulture Science 6 (1): 71−73.
Naserinasab F., Sahebani N., Etebarian H.R. 2011. Biological control of Meloidogyne javanica by Trichoderma harzianum BI and salicylic acid on tomato. African Journal of Food Science 5 (3): 276−280.
Oyeleke S.B., Oyewole O.A., Dagunduro J.N. 2011. Effect of herbicide (pendimethalin) on soil microbial population. Journal of Food and Agriculture Science 1 (3): 40−43.
Peer F.A., Hassan B., Lone B.A., Qayoom S., Ahmad L., Khanday B.A., Ssingh P., Singh G. 2013. Effect of weed control methods on yield and yield attributes of soybean. African Journal of Agricultural Research 8 (48): 6135−6141. DOI: 10.5897/AJAR11.1172.
Rehman S.U., Lawrence R., Kumar E.J., Badri Z.A. 2012. Comparative efficacy of Trichoderma viride, T. harzianum and carbendazim against damping-off disease of cauliflower caused by Rhizoctonia solani Kuehn. Journal of Biopesticides 5 (1): 23−27.
Saber W.I.A., Abd El-Hai K.M., Ghoneem K.M. 2009. Synergistic effect of Trichoderma and Rhizobium on both biocontrol of chocolate spot disease and induction of nodulation, physiological activities and productivity of Vicia faba. Research Journal of Microbiology 4: 286−300. DOI: 10.3923/jm.2009.286.300.
Saxena D., Tewari A.K., Rai D. 2014. The in vitro effect of some commonly used fungicides, insecticides and herbicides for their compatibility with Trichoderma harzianum PBT23. World Applied Sciences Journal 31 (4): 444−448. DOI: 10.5829/idosi.wasj.2014.31.04.78.
Singh M., Sharma O.P., Bhagat S. 2014. Compatibility of promising Trichoderma spp. with pesticides. Pesticide Research Journal 26 (2): 217−220.
Southey J.F. 1970. Laboratory Methods for Work with Plant and Soil Nematodes. Technical Bulletin of Ministry of Agriculture, Fishers and Food, London, 148 pp.
Snedecor G.W., Cochran W.G. 1980. Statistical Methods. 5th ed. Iowa State University Press, Ames, Iowa, USA, 593 pp.
Stoddard F.L., Nicholas A.H., Rubiales D., Thomas J., Villegas-Fernăndez A.M. 2010. Integrated pest management in faba bean. Field Crops Research 115 (3): 308−318. DOI: 10.1016/j.fcr.2009.07.002.
Taylor A.L., Sasser J.N. 1978. Biology, identification and control of root − knot nematodes (Meloidogyne species). IMP, North Carolina State University Graphics, Raleigh, USA, 111 pp.
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