ORIGINAL ARTICLE
Genotypic differences between isolates of Phytophthora cinnamomi Rands and P. citricola Sawada obtained from twelve nursery plant species
1
Research Institute of Pomology and Floriculture
Pomologiczna 18, 96-100 Skierniewice, Poland
2
Central Laboratory of State Plant Health and Seed Inspection Service
Żwirki i Wigury 73, 87-100 Toruń, Poland
Corresponding author
Katarzyna Wiejacha
Research Institute of Pomology and Floriculture Pomologiczna 18, 96-100 Skierniewice, Poland
Research Institute of Pomology and Floriculture Pomologiczna 18, 96-100 Skierniewice, Poland
Journal of Plant Protection Research 2004;44(3):189–198
KEYWORDS
Phytophthora cinnamomi RandsPhytophthora citricola Sawadageneticdistancerandom amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR)inter-simple sequence repeats - polymerase chain reaction (ISSR-PCR)
TOPICS
ABSTRACT
Genotypic differentiation among 10 isolates of Phytophthora cinnamomi Rands and 24 isolates of Phytophthora citricola Sawada from 12 different plant species
grown in Polish ornamental nurseries was determined. DNA was extracted from pure pathogen cultures and amplified by the PCR technique using ISSR and RAPD primers. 9 primers were used to amplify P. cinnamomi and 8 to amplify
P. citricola DNA. The analyzed amplification products were between 300 and 2300 bp. The genotypical differentiation was from 17 to 35% in P. cinnamomi and from 10 to 60% in P. citricola. Isolates from host plants of the same family showed, with some exceptions, similar levels of differentiation.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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