ORIGINAL ARTICLE
Molecular identification of mating type genes in asexually reproducing Fusarium oxysporum and F.culmorum
1
Department of Phytopathology, Poznań University of Life Sciences
Dąbrowskiego 159, 60-594 Poznań, Poland
Corresponding author
Lidia Irzykowska
Department of Phytopathology, Poznań University of Life Sciences Dąbrowskiego 159, 60-594 Poznań, Poland
Department of Phytopathology, Poznań University of Life Sciences Dąbrowskiego 159, 60-594 Poznań, Poland
Journal of Plant Protection Research 2011;51(4):405-409
KEYWORDS
TOPICS
ABSTRACT
Sexually (homothallic and heterothallic) and asexually reproducing species belong to the
Fusarium
genus.
So far, there is
no known sexual stage of the
F. oxysporum
Schlechtend.: Fr. and
F. culmorum
(W.G. Smith) Sacc. Knowing the reproduction mode is
important for the design of successful control strategies, since they are different for clonally and sexually reproducing organisms. In
examined sets of asexual
F. oxysporum
and
F. culmorum
isolates, the DNA sequences of mating type genes (idiomorphs
MAT-1
and
MAT-2
) were identified.
MAT-1
sequence was detected for 33 and 40% of
F. oxysporum
and
F. culmorum
isolates, respectively. For the
remaining isolates a sequence specific for
MAT-2
was amplified
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (27)
1.
Arie T., Kaneko I., Yoshida T., Noguchi M., Nomura Y., Yamaguchi I. 2000. Mating-type genes from asexual phytopathogenic ascomycetes Fusarium oxysporum and Alternaria alternata. Mol. Plant Microbe 13: 1330–1339.
3.
Christiansen S.K., Wirsel S., Yun S.H., Yoder O.C., Turgeon B.G. 1998. The two Cochliobolus mating-type genes are conserved among species bur one of them is missing in C. victoriae. Mycol. Res. 102 (8): 919–929.
4.
Dyer P.S., Furneaux P.A., Douhan G., Murray T.D. 2001. A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis.Fungal Genet. Biol. 33 (3): 173–180.
5.
Gerlach W., Nirenberg H. 1982. The Genus Fusarium. A Pictorial Atlas. Berlin-Dahlem (Germany): Mitt. Biol. Bundesanst. Land.-u. Forstwirtschaft. Berl.-Dahlem., 407 pp.
6.
Irzykowska L., Baturo A. 2008. Genetic polymorphism of Fusarium culmorum isolates originating from roots and stem bases of barley. J. Plant Protection Res. 48 (3): 303–312.
7.
Irzykowska L., Bocianowski J. 2008. Genetic variation, pathogenicity and mycelial growth rate differentiation between Gaeuanno tceenrainievar. Tritici isolates derived from winter and spring wheat. Ann. Appl. Biol.152: 369–375.
8.
Karényi Z., Moretti A., Waalwijk C., Olah B., Hornok L. 2004. Mating type sequences in asexually reproducing Fusarium species. Appl. Environ. Microb. 70 (8): 4419–4423.
9.
Karényi Z., Zeller K., Hornok L., Leslie J.F. 1999. Molecular standardization of mating type terminology in the Gibberella fujikuroi species complex. Appl. Environ. Microb. 65 (9):4071–4076.
10.
Kistler H.C. 1997. Genetic Diversity in the Plant-Pathogenic Fungus Fusarium oxysporum. Phytopathology 87: 474–479.
11.
Kothe E. 2001. Mating-type genes for basidiomycete strain improvement in mushroom farming. Appl. Microbiol. Biotechnol. 56: 602–612.
12.
Kwasna H., Chelkowski J., Zajkowski P. 1991. Fungi (Mycota) XXII. Warsaw (Poland): Polish Academy of Sciences, 136 pp.
13.
Logrieco A., Bottalico A., Mulé G., Moretti A., Perrone G. 2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. Eur. J. Plant. Pathol. 109 (7): 645–667.
14.
Nicolaisen M., Justesen A.F., Thrane U., Skouboe P., Holmstrøm K. 2005. An oligonucleotide miccroarray for the identification and differentiation of trichothecene producing and non-producing Fusarium species occurring on cereal grain. J. Microbiol. Meth. 62: 57–69.
15.
McDonald B.A., McDermott J.M. 1993. Population genetics of plant pathogenic fungi. Bioscience 43: 311–319.
16.
Mulè G., Susca A., Stea G., Moretti A. 2004a. Specific detection of the toxigenic species Fusarium proliferatum and F. oxysporum from asparagus plants using primers based on calmodulin gene sequences. FEMS Microbiol. Lett. 230 (2): 235–240.
17.
Mulè G., Susca A., Stea G., Moretti A. 2004b. Corrigendum to ‘Specific detection of the toxigenic species Fusarium profileratum and F. oxysporum from asparagus plants using primers based on calmodulin gene sequences’. FEMS Microbiol. Lett. 232, p. 229.
18.
O’Donnell K., Ward T.J., Geiser D.M., Kistler H.C., Aoki T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes support formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genet. Biol. 41 (6): 600–623.
19.
O’Donnell K., Gueidan C., Sink S.L., Johnston P.R., Crous P., Glenn A.E., Riley R.T., Zitomer N.C., Colyer P., Waalwijk C., Van Der Lee T., Moretti A., Kang S., Kim H., Geiser D.M., Juba J., Baayen R.P., Cromey M.G., Bithel S., Sutton D.A., Skovgaard K., Ploetz R., Kistler H.C., Elliott M., Davis M., Sarver B.A. 2009. A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genet. Biol. 46 (12): 936–948.
20.
Pöggeler S. 2001. Mating-type genes for classical strain improvements of ascomycetes. Appl. Microbiol. Biotechnol. 56: 589–601.
21.
Schilling A.G., Möller E.M., Geiger H.H. 1996. Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, .graminearum and F. avenaceum.Phytopathology 86: 515–522.
22.
Taylor J., Jacobson D.J, Fisher M.C. 1999. The evolution of asexual fungi: Reproduction, speciation and classification. Annu. Rev. Phytopathol. 37: 197–246.
23.
Turgeon B.G. 1998. Application of mating type gene technology to problems in fungal biology. Annu. Rev. Phytopathol. 36: 115–137.
24.
Turgeon B.G., Yoder O.C. 2000. Commentary. Proposed nomenclature for mating type genes of filamentous ascomycetes .Fungal Genet. Biol. 31 (1): 1–5.
25.
Waskiewicz A., Golinski P., Karolewski Z., Irzykowska L., Bocianowski J., Kostecki M., Weber Z. 2010. Formation of fumonisins and other secondary metabolites by Fusarium oxysporum and F. proliferatum- a comparative study. Food Addit. Contam. 27: 608–615.
26.
Waskiewicz A., Irzykowska L., Karolewski Z., Bocianowski J., Golinski P., Weber Z. 2009. Mycotoxins biosynthesis by Fusarium oxysporum and F. proliferatum isolates of asparagus origin. J. Plant Protection Res. 49 (4): 369–372.
27.
Yun S-H., Arie T., Kaneko I., Yoder O.C., Turgeon B.G. 2000. Molecular organization of mating type loci in heterothalic, homothalic and asexual Gibberella/Fusarium species. Fungal Genet. Biol. 31 (1): 7–20.
Share
RELATED ARTICLE
| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
