ORIGINAL ARTICLE
 
HIGHLIGHTS
  • We embarked on an in-depth phytochemical characterization of the various components present in PVEO.
  • We utilized the High-Performance Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry (HS-SPME-GC-MS) technique.
KEYWORDS
TOPICS
ABSTRACT
The objective of this investigation was to assess the chemical makeup of essential oil derived from Ptychotis verticillata (PVEO), and to examine its antifungal, insecticidal, and repel- lent properties. PVEO was extracted through hydrodistillation, and its volatile constituents were analyzed using Headspace Solid-Phase Microextraction Gas Chromatography-Mass Spectrometry. Qualitative and quantitative evaluation of antifungal activity was carried out using the agar diffusion method and the minimum inhibitory concentration (MIC) test against Candida glabrata, Saccharomyces cerevisiae, Aspergillus niger and Penicillium digitatum. We evaluated the repellent potential, as well as the contact and inhalation tox- icity of PVEO against Callosobruchus maculatus. The results of the study indicated that the essential oil of P. verticillata was composed mainly of γ-Terpinen (25.86%), β-Cymene (18.70%) O-Cymen-5-ol (16.78) and α-Pinene (12.13%). PVEO showed potent antifungal activity against all strains tested. The results of insecticidal activity of this essential oil were promising in adult C. maculatus. At a dose of 20 µl · dm–3 of air, EO caused maximum mortality with an LC50 value of 5.64 µl · dm–3 for the inhalation test and 3.4 µl · dm–3 for the contact test. In addition, a significant decrease in the number of eggs laid and adult emer- gence was observed as EO doses increased, reaching a reduction of around 95% at a dose of 20 µl · dm–3 of air. In terms of repellent activity, PVEO also showed encouraging results. It demonstrated an average repellent activity of around 92 ± 10.95%. Furthermore, molecular docking simulations corroborated the in vitro results and demonstrated that specific p-Menthen-3-one compounds formed more robust hydrogen bonding interactions with the target receptors. These experiments underscore PVEO’s effectiveness as a fungicide against the tested fungal strains, demonstrating its role as a bio-insecticide against C. macu latus adults, and its potential as an appealing repellent. This suggests that PVEO could serve as a valuable alternative within integrated pest management strategies.
ACKNOWLEDGEMENTS
The authors express their gratitude to the Moroccan Ministry of Higher Education, Scientific Research and Innovation, and the European Union for supporting this work under the PRIMA Programme (OLI4FOOD, ID No 1845), as well as for the support provided by the researcher support project (PNURSP2024R158) of the Princess Nourah Bint Abdulrahman University in Riyadh, Saudi Arabia.
RESPONSIBLE EDITOR
Jolanta Kowalska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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