Potential of endochitinase gene to control Fusarium wilt and early blight disease in transgenic potato lines
Saman Riaz 1, D
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Centre of Excellence in Molecular Biology, University of the Punjab, Lahore-Pakistan, Pakistan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2019-01-31
Acceptance date: 2019-08-21
Online publication date: 2019-10-09
Corresponding author
Bushra Tabassum   

Centre of Excellence in Molecular Biology, University of the Punjab, Lahore-Pakistan, Pakistan
Journal of Plant Protection Research 2019;59(3):376-382
Potato (Solanum tuberosum L.), an important food crop in the world, is susceptible to many fungal pathogens including Alternaria solani and Fusarium oxysporum causing Fusarium wilt and early blight diseases. Mycoparasitic fungi like Trichoderma encode chitinases, cell wall degrading enzymes, with high antifungal activity against a wide range of phytopathogenic fungi. In this study, a binary vector harboring endochitinase gene of ~1,000 bp was constructed and used to transform potato nodes through Agrobacterium-mediated transformation. Out of several primary transformants, two transgenic potato lines were verified for transgene insertion and integration by Southern blot. In a pot experiment for Fusarium resistance, the transgenic potato lines didn’t show any symptoms of disease, instead they remained healthy post infection. The transgenic potato lines exhibited 1.5 fold higher mRNA expression of endochitinase at 7 days as compared to 0 day post fungus inoculation. It was evident that the mRNA expression decreased over days of inoculation but was still higher than at 0 day and remained stable upto 30 days post inoculation. Similarly, for A. solani infection assay, the mRNA expression of the endochitinase gene was 3 fold higher 7 days post inoculation compared to expression at 0 day. Although the expression decreased by1.2 fold during subsequent days post infection, it remained stable for 30 days, suggesting that protection in transgenic potato plants against fungal pathogens was achieved through an increase in endochitinase transcript.
The authors have declared that no conflict of interests exist.
Christou P., Twyman R.M. 2004. The potential of genetically enhanced plants to address food insecurity. Nutrition Research Reviews. 17 (1): 23–42. DOI: 10.1079/NRR200373.
de las Mercedes Dana M., Pintor-Toro J.A., Cubero B. 2006. Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents. Plant Physiology 142 (2): 722–730. DOI: https://doi.org/10.1104/pp.106....
Emani C., Garcia J.M., Lopata-Finch E., Pozo M.J., Uribe P., Kim D.J., Rathore K.S. 2003. Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens. Plant Biotechnology Journal 1 (5): 321–336. DOI: 10.1046/j.1467-7652.2003.00029.x.
Esfahani K., Motallebi M., Zamani M.R., Hashemi S.H., Jourabchi E. 2010. Transformation of Potato (Solanum tuberosum cv. Savalan) by Chitinase and β-1, 3-Glucanase Genes of Myco-Parasitic Fungi Towards Improving Resistance to Rhizoctonia solani AG-3. Iranian Journal of Biotechnology 8 (2): 73–81.
Gentile A., Deng Z., La Malfa S., Distefano G., Domina F., Vitale A., Tribulato E. 2007. Enhanced resistance to Phomatra cheiphila and Botrytis cinerea in transgenic lemon plants expressing a Trichoderma harzianum chitinase gene. Plant Breeding 126 (2): 146–151. DOI: https://doi.org/10.1111/j.1439....
Gokul B., Lee J.H., Song K.B., Rhee S.K., Kim C.H., Panda T. 2000. Characterization and applications of chitinases from Trichoderma harzianum-A review. Bioprocess Engineering 23: 691–694. DOI: https://doi.org/10.1007/s00449....
Harighi M.J., Motallebi M., Zamani M.R., 2006. Purification of chitinase 42 from Trichoderma atroviride PTCC5220. Iranian Journal of Biotechnology 19: 203–214.
Hermosa M., Grondona I., Iturriaga Et, Diaz-Minguez J., Castro C., Monte E., Garcia-Acha I. 2000. Molecular characterization and identification of biocontrol isolates of Trichoderma spp. Applied and Environmental Microbiology 66 (5): 1890–1898. DOI: 10.1128/aem.66.5.1890-1898.2000.
Jabeen N., Chaudhary Z., Gulfraz M., Rashid H., Mirza B. 2015. Expression of rice chitinase gene in genetically engineered tomato confers enhanced resistance to Fusarium wilt and early blight. Journal of Plant Pathology 31 (3): 252–258. DOI: 10.5423/PPJ.OA.03.2015.0026.
Jeger M., Hide G., Van den Boogert P, Termorshuizen A., Van Baarlen P. 1996. Pathology and control of soil-borne fungal pathogens of potato. Potato Research 39 (3): 437–469.
Keen N. 2000. A century of plant pathology: A retrospective view on understanding host-parasite interactions. Annual Review of Phytopathology 38 (1): 31–48. DOI: https://doi.org/10.1146/annure....
Khan A., Nasir I.A., Tabassum B., Aaliya K., Tariq M., Rao A.Q. 2017. Expression studies of chitinase gene in transgenic potato against Alternaria solani. Plant Cell, Tissue and Organ Culture 128 (3): 563–576. DOI: https://doi.org/10.1007/s11240....
Kumar J., Rawat L. 2011. The threat of plant diseases to food security. Proceedings of the 25th Training on Quality Management and Plant Protection Practices for Enhanced Competitiveness in Agricultural Export, New Dehli: Centre of Advanced Faculty Training in Plant Pathology.
Mora A.A., Earle E.D. 2001. Resistance to Alternaria brassicicola in transgenic broccoli expressing a Trichoderma harzianum endochitinase gene. Molecular Breeding 8: 1–9.
Lorito M., Woo S.L., Fernandez I.G., Colucci G., Harman G.E., PintorToro J.A., Filippone E., Muccifora S., Lawrence C.B., ZoinaTuzun S., Scala F. 1998. Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens. Proceedings of the National Academy of Sciences of the United States of America. 95: 7860–7865. DOI: 10.1073/pnas.95.14.7860.
Murashige T., Skoog F. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15 (3): 473–497. DOI: https://doi.org/10.1111/j.1399....
Oerke E.C., Dehne H.W. 2004. Safeguarding production-losses in major crops and the role of crop protection. Crop Protection 23 (4): 275–285. DOI: https://doi.org/10.1016/j.crop....
Pavlik M., Jandurová O.M. 2000. Fungicides cytotoxicity expressed in male gametophyte development in Brassica campestris after in vitro application of converted field doses. Environmental and Experimental Botany 44 (1): 49–58. DOI: https://doi.org/10.1016/S0098-....
Perrakis A., Tews I., Dauter Z., Oppenheim A.B., Chet I., Wilson K.S., Vorgias C.E. 1994. Crystal structure of a bacterial chitinase at 2.3 Å resolution. Structure 2 (12): 1169–1180. DOI: https://doi.org/10.1016/S0969-....
Pinnamaneni R., Kalidas P., Rao K.S. 2010. Cloning and expression of Bbchit1 gene of Beauveria bassiana. The Open Entomology Journal 4: 30–35. DOI: 10.2174/1874407901004010030.
Savary S., Ficke A., Aubertot J.N., Hollier C. 2012. Crop losses due to diseases and their implications for global food production losses and food security. Food Security 4 (4): 519–537. DOI: https://doi.org/10.1007/s12571....
Shibuya N., Minami E. 2001. Oligosaccharide signaling for defense responses in plant. Physiological and Molecular Plant Pathology 59 (5): 223–233. DOI: https://doi.org/10.1006/pmpp.2....
Solgi T., Moradyar M., Zamani M.R., Motallebi M. 2015. Transformation of Canola by Chit33 Gene towards Improving Resistance to Sclerotinia sclerotiorum. Plant Protection Science 51 (1): 6–12. DOI: https://doi.org/10.17221/83/20....
Suzuki K., Taiyoji M., Sugawara N., Nikaidou N., Henrissat B., Watanabe T. 1999. The third chitinase gene (chiC) of Serratia marcescens 2170 and the relationship of its product to other bacterial chitinases. Biochemical Journal 343 (3): 587–596. DOI: https://doi.org/10.1042/bj3430....
Tabassum B., Nasir I.A., Khan A., Aslam U., Tariq M., Shahid N., Husnain T. 2016. Short hairpin RNA engineering: In planta gene silencing of potato virus Y. Crop Protection 86: 1–8. DOI: https://doi.org/10.1016/j.crop....
Tariq M., Khan A., Tabassum B., Toufiq N., Bhatti M.U, Riaz S., Nasir I.A., Husnain T. 2018. Antifungal activity of chitinase II against Colletotrichum falcatum Went. causing red rot disease in transgenic sugarcane. Turkish Journal of Biology 42 (1): 45–53. DOI:10.3906/biy-1709-17.
Toufiq N., Tabassum B., Bhatti M.U., Khan A., Tariq M., Shahid N., Nasir I.A., Husnain T. 2017. Improved antifungal activity of barley derived chitinase I gene that overexpress a 32kDa recombinant chitinase in E. coli host. BrazilianJournal of Microbiology 49 (2): 414–421. DOI: 10.1016/j.bjm.2017.05.007.
van Aalten D.M., Synstad B., Brurberg M.B., Hough E., Riise B.W., Eijsink V.G., Wierenga R.K. 2000. Structure of a two-domain chitotriosidase from Serratia marcescens at 1.9-A resolution. Proceedings of the National Academy of Sciences of the United States of America 97(11): 5842–5847. DOI: 10.1073/pnas.97.11.5842.
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