Susceptibility of winter triticale cultivars to Rhizoctonia cerealis (Sharp eyespot) and R. solani
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University of Technology and Life Sciences Department of Phytopathology and Molecular Mycology Kordeckiego 20, 85-225 Bydgoszcz, Poland
Submission date: 2012-06-22
Acceptance date: 2012-08-23
Corresponding author
Grzegorz Lemańczyk
University of Technology and Life Sciences Department of Phytopathology and Molecular Mycology Kordeckiego 20, 85-225 Bydgoszcz, Poland
Journal of Plant Protection Research 2012;52(4):421-434
In the field study period from 2006 to 2010, the incidence and severity of sharp eyespot caused by Rhizoctonia were recorded on 36 cultivars of triticale at the milk ripe growth stage. Four localities in north-central Poland were included: Chrząstowo, Dębina, Kończewice and Minikowo. The susceptibility of the seedlings of 30 cultivars of triticale to R. cerealis (AG-D subgroup I) and R. solani (AG-5) was studied in the laboratory. There was much variation in incidence and severity of sharp eyespot between years and locations. The disease was most intense at Chrząstowo. At this location, the mean percentage of diseased stems on 28 cultivars was 2.6–35.7 (–55.0), and the mean disease index was 0.7–15.6 (–24.5), with the lowest and highest values in 2008 and 2009, respectively. At Minikowo, the disease was least intense. At this location, the mean percentage of diseased stems on 23 cultivars was 1.0–4.6 (–18.0), and the mean disease index was 0.3–1.4 (–6.3), with the lowest and highest values in 2006 and 2007, respectively. The cultivars with least intense disease were Tulus and Atletico (Chrząstowo), Grenado and Zorro (Dębina), Krakowiak and Tornado (Kończewice), and Woltario and Constans (Minikowo). The cultivars with most intense disease were Alekto (Chrząstowo), Baltiko (Dębina), Pawo (Kończewice) and Borwo (Minikowo). Mostly R. cerealis was isolated from the diseased stems; R. solani was isolated only sporadically. There was a wide variation in the susceptibility of triticale cultivars to Rhizoctonia. Most triticale seedlings inoculated with R. cerealis produced symptoms typical of sharp eyespot. Seedlings inoculated with R. solani formed extended lesions with no defined borders. Most symptoms developed on coleoptiles, with less symptoms on the leaves and the least on the roots. There was much variation in susceptibility of triticale cultivars to both Rhizoctonia species. Cultivars were grouped into six categories according to the intensity of seedling infection. Categories 1, 2 and 3, representing low, moderate and high susceptibility to R. cerealis, included 17, 10 and 3 cultivars, respectively. Categories 4, 5 and 6, representing low, moderate and high susceptibility to R. solani, included 3, 12 and 15 cultivars, respectively. Cultivars Baltiko and Zorro had low, and cv. Cultivo had high susceptibility to both Rhizoctonia species. No cultivar was resistant to Rhizoctonia. There was a positive correlation between infection by R. cerealis and R. solani. Infection of coleoptiles by R. cerealis or R. solani was significantly correlated with infection of leaves. No correlation between intensity of sharp eyespot on triticale plants in the field and on seedlings in controlled conditions was found.
The authors have declared that no conflict of interests exist.
Bandoni R.J. 1979. Safranin O as a rapid nuclear stain for fungi. Mycologia 71 (4): 873–874.
Bateman G.L., Edwards S.G., Marshall J., Morgan L.W., Nicholson P., Nuttall M., Parry D.W., Scrancher M., Turner A.S. 2000. Effects of cultivar and fungicides on stem-base pathogens, determined by PCR, and on diseases and yield of wheat. Ann. Appl. Biol.137 (3): 213–221.
Bockus W.W., Bowden R.L., Hunger R.M., Morrill W.L., Murray T.D., Smiley R.W. (eds.). 2010. Compendium of Wheat Diseases and Pests. 3rd ed. APS Press, St. Paul, MN, 171 pp.
Boerema G.H., Verhoeven A.A. 1977. Check-list for scientific names of common parasitic fungi. Series 26: Fungi on field crops: Cereals and grasses. Neth. J. Plant Pathol. 83 (5): 165–204.
Chen H.G., Cao Q.G., Xiong G.L., Li W., Zhang A.X., Yu H.S., Wang J.S. 2010. Composition of wheat rhizosphere antagonistic bacteria and wheat sharp eyespot as affected by rice straw mulching. Pedosphere 20 (4): 505–514.
Chen L., Zhang Z.Y., Liang H.X., Liu H.X., Du L.P., Xu H.J., Xin Z.Y. 2008. Overexpression of TiERF1 enhances resistance to sharp eyespot in transgenic wheat. J. Exp. Bot. 59 (15): 4195–4204.
Clarke G.M. 1984. Statistics and Experimental Design. Edward Arnold, London, 188 pp.
Clarkson J.D.S., Cook R.J. 1983. Effects of sharp eyespot on yield loss in winter wheat. Plant Pathol. 32 (4): 421–428.
Colbach H., Lucas P., Cavelier N., Cavelier A. 1997. Influence of cropping system on sharp eyespot in winter wheat. Crop Prot. 16 (5): 415–422.
Cromey M.G., Butler R.C., Boddington H.J., Moorhead A.R. 2002. Effects of sharp eyespot on yield of wheat (Triticum aestivum) in New Zealand. NZ J. Crop Hort. 30 (1): 9–17.
Cromey M.G., Butler R.C., Munro C.A., Shorter S.C. 2005. Susceptibility of New Zealand wheat cultivars to sharp eyespot. NZ Plant Prot.-SE. 58: 268–272.
Daamen R.A., Stol W. 1990. Surveys of cereal diseases and pests in theNetherlands. 2. Stem-base diseases of winter wheat. Neth. J. P1ant Pathol. 96 (5): 251–260.
Demirci E. 1998.Rhizoctonia species and anastomosis groups isolated from barley and wheat in Erzurum, Turkey.Plant Pathol.47 (1): 10–15.
Doyle J.J., Doyle J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12 (1): 13–15.Etheridge J.V., Davey L., Christian D.G. 2001. First report of Rhizoctonia cerealia causing sharp eyespot in Panicum virgatumin the UK. Plant Pathol. 50 (6): 807.
Gondko R., Zgirski A., Adamska M. 1994. Skala T (tenowa). p. 205–206. In: “Biostatystyka w zadaniach”. Wydawnictwo UŁ, Łódź, 532 pp.
Hamada M.S., Yin Y., Chen H., Ma Z. 2011. The escalating threat of Rhizoctonia cerealis, the causal agent of sharp eyespot in wheat. Pest Manag. Sci. 67 (11): 1411–1419.
Hoeven E.P. van der, Bollen G.J. 1980. Effect of benomyl on soil fungi associated with rye. 1. Effect on the incidence of sharp eyespot caused by Rhizoctonia cerealis. Neth. J. Plant Pathol. 86 (3): 163–180.
Johanson A., Turner H.C., McKay G.J., Brown A.E. 1998. A PCR-based method to distinguish fungi of the rice sheath-blight complex, Rhizoctonia solani, R. oryzae and R. oryzae-sativae. FEMS Microbiol. Lett. 162 (2): 289–294.
Kotwica K. 2008. Możliwości łagodzenia ujemnych skutków uprawy zbóż po sobie. [Possibilities of Alleviating Negative Effects of Cereal Growing after Each Other]. Rozprawy UTP, Bydgoszcz 129, 101 pp.
Kryuchkova L. 2000. Stem-base diseases of wheat in Ukraine. p. 113–118. In: “2000 BCPC Conference – Pests and Diseases 2000”. Proc. BCPC Conference – Pests & Diseases. Brighton, UK, 13–16 November 2000, 1297 pp.
Kurowski T.P. 2002. Studia nad chorobami podsuszkowymi zbóż uprawianych w wieloletnich monokulturach. [Studies on Root and Foot-Rot Diseases of Cereals Grown in Long-Term Monoculture]. Rozprawy i monografie UW-M Olsztyn 56, 86 pp.
Kurowski T.P., Adamiak E. 2007. Occurrence of stem base diseases of four cereal species grown in long-term monocultures. Pol. J. Natur. Sci. 22 (4): 574–583.
Kwaśna H., Bateman G.L., Ward E. 2010. Microbiota in wheat roots evaluated by cloning of ITS1/2 rDNA and sequencing. J. Phytopathol. 158 (4): 278–287.
Kwiatek M., Pankiewicz K., Wiśniewska H., Korbas M., Danielewicz J. 2012. Identification of Pch1 eyespot resistance gene in the collection of wheat lines (Triticum aestivum L.). J. Plant Prot. Res. 52 (2): 254–258.
Larkin R.P., Honeycutt C.W., Olanya O.M. 2011. Management of Verticillium wilt of potato with disease-suppressive green manures and as affected by previous cropping history. Plant Dis. 95 (5): 568–576.
Lemańczyk G. 2010a. Occurrence of sharp eyespot (Rhizoctonia cerealis) in winter triticale grown in some provinces of Poland. Phytopathologia 56: 27–38.
Lemańczyk G. 2010b. Occurrence of sharp eyespot in spring cereals grown in some regions of Poland. J. Plant Prot. Res. 50 (4): 505–512.
Lemańczyk G. 2012a. The role of the preceding crop and weed control in the transmission of Rhizoctonia cerealia and R. solani to winter cereals. J. Plant Prot. Res. 52 (1): 93–105.
Lemańczyk G. 2012b. Effects of farming system, chemical control, fertilizer and sowing density on sharp eyespot and Rhizoctonia spp. in winter wheat.J. Plant Prot. Res. 52 (4): 381–396.
Lemańczyk G., Sadowski Cz. 2002. Fungal communities and health status of roots of winter wheat cultivated after oats and oats mixed with other crops. BioControl47 (3): 349–361.
Li Z., Zhou M., Zhang Z., Ren L., Du L., Zhang B., Xu H., Xin Z. 2011. Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Funct. Integr. Genomic 11 (1): 63–70.
Lipps P.E., Herr L.J. 1982. Etiology of Rhizoctonia cerealia in sharp eyespot of wheat. Phythopathology 72 (12): 1574–1577.
Liu H.X., Xin Z.Y., Zhang Z.Y. 2011. Changes in activities of antioxidant-related enzymes in leaves of resistant and susceptible wheat inoculated with Rhizoctonia cerealis. Agric. Sci. China 10 (4): 526–533.
Matusinsky P., Mikolasova R., Klem K., Spitzer T., Urban T. 2008.The role of organic vs. conventional farming practice, soil management and preceding crop on the incidence of stem-base pathogens on wheat. J. Plant Dis. Prot. 115 (1): 17–22.
Mazzola M., Smiley R.W., Rovira A.D., Cook R.J. 1996. Characterization of Rhizoctonia isolates, disease occurrence and management in cereals. p. 259 267. In: “Rhizoctonia Species: Taxonomy, Molecular, Biological, Ecological, Pathology, and Disease Control” (B. Sneh, S. Jabaji-Hare, S. Neate, G. Dijst, eds.). Kluwer Academic Publishers, Dordrecht, The Netherlands, 584 pp.
McCall W.A. 1922. How to Measure in Education. Macmillan Company, New York, NY, USA, 416 pp.
Nicholson P., Parry D.W. 1996. Development and use of a PCR as say to detect Rhizoctonia cerealis, the cause of sharp eyespot in wheat. Plant Pathol. 45 (5): 872–883.
Nicholson P., Turner A.S., Edwards S.G., Bateman G.L., Morgan L.W., Parry D.W., Marshall J., Nuttall M. 2002. Development of stem-base pathogens on different cultivars of winter wheat determined by quantitative PCR. Eur. J. Plant Pathol. 108 (2): 163–177.
Ogoshi A., Cook R.J., Bassett E.N. 1990. Rhizoctonia species and anastomosis groups causing root rot of wheat and barley in the Pacific Northwest. Phytopathology 80 (9): 784–788.
Okubara P.A., Schroeder K.L., Paulitz T.C. 2008. Identification and quantification of Rhizoctonia solani and R. oryzae using real-time polymerase chain reaction. Phytopathology 98 (7): 837–847.
Polley R.W., Thomas M.R. 1991. Surveys of diseases of winter wheat in England and Wales, 1976–1988. Ann. Appl. Biol.119 (1): 1–20.
Prew R.D., Macintosh A.H.1975. Effects of benomyl and other fungicides on take-all, eyespot and sharp eyespot diseases of winter wheat. Plant Pathol.24 (2): 67–71.
Ray R.V., Jenkinson P., Edwards S.G. 2004. Effects of fungicides on eyespot, caused predominantly by Oculimacula acuformis, and yield of early-drilled winter wheat. Crop Prot. 23 (12): 1199–1207.
Ren L.J., Zhang X., Zhou M.P., Lu W.Z., Ma H.X. 2007. QTL analysis of sharp eyespot (Rhizoctonia cerealis) and Fusarium Head Blight in wheat. J. Triticeae Crop 3: 416–420. http://en.cnki.com.cn/Article_... CJFDTOTAL-MLZW200703011.htm Accessed: April 20, 2012.
Rossi V., Cervi C., Chiusa G., Languasco L. 1995. Fungi associated with foot rots on winter wheat in northwest Italy. J. Phytopathol. 143 (2): 115–119.
Sneh B., Burpee L., Ogoshi A. 1991. Identification of Rhizoctonia Species.APS Press, St. Paul, MN, USA, 133 pp.
Tewoldemedhin Y.T., Lamprecht S.C., McLeod A., Mazzola M. 2006. Characterization of Rhizoctonia spp. recovered from crop plants used in rotational cropping systems in the Western Cape province of South Africa. Plant Dis. 90 (11): 1399–1406.
Toda T., Hyakumachi M., Suga H., Kageyama K., Tanaka A., Tani T. 1999. Differentiation of Rhizoctonia AG-D isolates from turfgrass into subgroups I and II based on rDNA and RAPD analyses. Eur. J. Plant Pathol. 105 (9): 835–846.
Tokarski P. 2011. Pszenżyto ozime. p. 106–117. In: “Lista opisowa odmian. Rośliny rolnicze. Cz. 1. Zbożowe” (J. Zych, ed.). COBORU, Słupia Wielka, 170 pp.
Tunali B., Nicol J.M., Hodson D., Uçkun Z., Büyük O., Erdurmuş D., Hekimhan H., Aktaş H., Akbudak M.A., Bağcı S.A. 2008. Root and crown rot fungi associated with spring, facultative, and winter wheat in Turkey. Plant Dis. 92 (9): 1299–1306.
Wachowska U. 2000. Susceptibility of cereals and other crops to Rhizoctonia cerealis. Phytopathol. Pol. 20: 59–66.
Wardlaw A.C. 2000. Practical Statistics for Experimental Biologists. 2nd ed. John Wiley and Sons, New York, 250 pp.
Wenzel H. 1948. Zur Erfassung des Schadenausmasses in Pflanzenschutzversuchen. Pflanzenschutz-Ber. 15: 81–84.
Zadoks J.C., Chang T.T., Konzak C.F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14 (6): 415–421.
Zhalieva L.D. 2008. Changes in a pathogenic complex of major pathogens causing root rot on grain cereals in conditions of Krasnodar. p. 105–106. In: Abstracts of Int. Conference on Information Systems of Diagnostics, Monitoring and Forecasting the Major Weed Plants, Pests and Diseases of Agricultural Crops. St. Petersburg/Pushkin, Russia, 12–16 May 2008, 120 pp.
Żółtańska E. 2005. The effect of previous crop and weather conditions on the incidence of stem base diseases in winter wheat. J. Plant Prot. Res. 45 (1): 37–40.
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