Temperature and pH impact on the mycelium growth of Mycogone perniciosa and Verticillium fungicola isolates derived from Polish and foreign mushroom growing houses
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Poznań University of Life Sciences Department of Vegetable Crops, Dąbrowskiego 159, 60-594 Poznań, Poland
Poznań University of Life Sciences Department of Plant Protection Methods, Zgorzelecka 4, 60-198 Poznań, Poland
Corresponding author
Marek Siwulski
Poznań University of Life Sciences Department of Vegetable Crops, Dąbrowskiego 159, 60-594 Poznań, Poland
Journal of Plant Protection Research 2011;51(3):268-272
The research project aimed at determining the effect of incubation temperature and pH of the agar medium on the mycelium growth of Mycogone perniciosa (Magnus) Delacroix and Verticillium fungicola (Preuss) Hassebrauk in in vitro conditions. Ten M. perni- ciosa and ten V. fungicola isolates were applied. Mycelium growth of the above-mentioned isolates was studied on PDA agar medium (Merck) enriched with an extract from mushroom hyphae, at temperatures ranging from 15 to 30°C and pH from 5.5 to 7.5. Incubation temperature was found to affect the mycelium growth rate of the examined pathogens. Mycelium growth in all M. perniciosa and V. fungicola isolates was the fastest at the temperature of 25°C, while a temperature of 15°C inhibited their growth significantly. Mycelium growth of the examined pathogens also depended on medium pH. M. perniciosa mycelium developed best at pH = 5.5, whereas at the same medium pH V. fungicola mycelium exhibited the worst growth.
The authors have declared that no conflict of interests exist.
Bech K., Jacobsen B.D., Kovacs G., Grabbe K., Hilber O. 1989. Investigations on the influence of temperature on growth and spore formation of Mycogone perniciosa and Verticillium fungicola, two pathogenic fungi of the cultivated mushroom. Mush. Sci. 12 (2): 739–751.
Fletcher J.T., Gaze R.H. 2008. Mushroom Pest and Disease Control. Manson Publishing, London, UK, 192 pp.
Gams W., van Zaayen A. 1982. Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. I. Taxonomy. Neth. J. Plant Pathol. 88 (2): 57–78.
Gea F.J., Tello J.C., Navarro M.J. 2003. Occurrence of Verticillium fungicola var. Fungicola on Agaricus bitorquis mushroom crops in Spain. J. Phythopathol. 151 (2): 98–100.
Geels F.P., van de Geijn J., Rutjens A.J. 1988. Pests and diseases. p. 361–422. In: “The Cultivation of Mushrooms” (L.J.LD. van Griensven, ed.). Horst, Netherlands, 515 pp.
Glamoclija J., Sokovic M., Ljaljevic-Grbic M., Vukojevic J., Milenkovic I., van Griensven L. 2008. Morphological characteristics and mycelial compatibility of different Mycogone perniciosa isolates. J. Microsc. 232 (3): 489–492.
Juarez del Carmen S., Largeteau-Mamoun M.L., Rousseau T., Regnault-Roger C., Savoie J.M. 2002. Genetic and physiological variation in isolates of Verticillium fungicola causing dry bubble disease of the cultivated button mushroom, Agaricus bisporus. Mycol. Res. 106 (8): 1163–1170.
Lambert E.B. 1930. Studies on the relation of temperature to the growth, parasitism, thermal death points, and control of Mycogone perniciosa.Phytopathology 20 (1): 75–83.
Maszkiewicz J., Dmowska E., Ignatowicz S., Lewandowski M., Szymański J. 2006. Ochrona Pieczarki. Praca zbiorowa pod red. J. Maszkiewicza. Hortpress, Warszawa, 144 pp.
Meyer L., Korsten L. 2008. A nested PCR for the detection of Mycogone perniciosa causing wet bubble disease of white button mushrooms. Mush. Sci. 17 (1): 554–564.
Nair N.G., Macauley B.J. 1987. Dry bubble disease of Agaricus Bisporus and A. bitorquis, and its control by prochlorazmanganese complex. New Zealand J. Agri. Res. 30 (1): 107–116.
Sharma V.P., Singh Ch. 2003. Biology and control of Mycogone perniciosaMagn. causing wet bubble disease of white button mushroom. J. Mycol. Plant Pathol. 33 (2): 257–264.
Tan Q., Wang l., Wang Y., Wang J. 1994. A study on biological characteristics in isolates of Mycogone perniciosa Magn. Acta Edulis Fungi 4 (2): 1996–2001.
Thapa C.D., Jandaik C.L. 1986. Spore germination behavior of Verticillium fungicola(Preuss) Hassebr. under different environmental conditions. p. 405–410. In: Proc. Int. Symp. Sci. Technical Aspects Cult. of Edible Fungi, Pennsylvania State University, PA, USA, 15–17 July 1986.
Umar M.H., Geels F.P., van Griensven L.J.LD. 2000. Pathology and pathogenesis of Mycogone perniciosa infection of Agaricus bisporus. p. 561–567. In: Proc. 15th Int. Cong. Sci. and Cult. of Edible Fungi, vol. 2, Maastricht, Netherlands. 15–19 May 2000.
van Zaayen A., Gams W. 1982. Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. II. Pathogenicity. Neth. J. Plant Path. 88 (2): 143–154.
Zare R., Gams W. 2008. A revision of the Verticillium fungicola species complex and its affinity with the genus Lecanicillium. Mycol. Res. 112 (7): 811–824.
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