Capsaicin as an organophosphate synergist against Colorado potato beetle (Leptinotarsa decemlineata Say)
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Department of Animal Toxicology, Institute of General and Molecular Biology Nicolaus Copernicus University Gagarina 9, 87-100 Toruń, Poland
Justyna Maliszewska
Department of Animal Toxicology, Institute of General and Molecular Biology Nicolaus Copernicus University Gagarina 9, 87-100 Toruń, Poland
Journal of Plant Protection Research 2012;52(1):28–34
The aim of the presented study was to assess the applicability of capsaicin as a synergist for organophosphate insecticide against the Colorado potato beetle ( Leptinotarsa decemlineata Say). We recorded the death rate, behavioral thermoregulation and metabolic rate of the examined insects after intoxication with capsaicin and organophosphate pesticide. The results obtained showed that the tested substances changed the thermal preferences of the potato beetle, causing the beetles to prefer a decrease in temperature in the thermal gradient system. We also observed an increase in the metabolic rate of the beetles intoxicated with capsaicin and organophosphate insecticide. The addition of capsaicin in a concentration of 10 –7 M (dissolved in alcohol) to insecticide resulted in an increase in the death rate of the insects by 24% in 35°C and 73% in 15°C, in comparison to organophosphate alone. Our results show that capsaicin in low concentrations may be an efficient synergist for organophosphate insecticides against the Colorado potato beetle.
The authors have declared that no conflict of interests exist.
Alyokhin A., Baker M., Mota-Sanchez D., Dively G., Grafius E. 2008. Colorado potato beetle resistance to insecticides. Am. J. Pot. Res. 85: 395–413.
Barčić J.I., Bažok R., Bezjak S., Čuljak T.G., Barčić J. 2006. Combinations of several insecticides used for integrated control of Colorado potato beetle (Leptinotarsa decemlineata Say, Coleoptera: Chrysomelidae). J. Pest. Sci. 79: 23–232. DOI: 10.1007/s10340-006-0138-5.
Ben-Ari Y. 2002. Excitatory actions of GABA during development: the nature of the nurture. Nat. Rev. Neurosci. 3: 728–739.
Błasiak J. 1996. Oddziaływanie Insektycydów Fosforoorganiznych z Błonami Biologicznymi. [The effect of organophosphate insecticides on biological membranes].Wyd. Uniwersytetu Łódzkiego, Łódź: 22–30.
Gökçe A., Isaacs R., Whalon M.E. 2006. Behavioural response of Colorado potato beetle (Leptinotarsa decemlineata) larvae to selected plant extracts. Pest. Manage. Sci. 62: 1052–1057.
Grajpel B., Kiełbasiewicz E., Stankiewicz M., Rogozińska E., Olszak R., Tęgowska E. 2001. Effect of indoxacarb and betacyflutrin on insects thermal behavior, metabolism and muscle resting potential. 10th Int. Symp. Molecular and Physiological Aspects of Regulatory Processes of the Organism. Cracow, 5–6.06.2001: 113–115.
Jokanović M. 2001. Biotransformation of organophosphorus compounds. Toxicology 166: 139–160.
Kobayashi A., Osaka T., Namba Y., Inoue S., Lee T., Kimura S. 1998. Capsaicin activates heat loss and heat production simultaneously and independently in rats. Am. J. Physiol. Regulatory Integrative Comp. Physiol. 275: 92–98.
Kozłowski J., Kałuski T., Jaskulska M. 2008. Badania laboratoryjne nad ograniczeniem uszkodzeń roślin rzepaku przez ślinika luzytańskiego (Arion lusitanicus Mabille). [Laboratory studies on reduction damage of oilseed rape plants caused byArion lusitanicus Mabille]. Prog. Plant Protection/Post. Ochr. Roślin 48 (3): 889–892.
Lundbæk J.A., Birn P., Tape S.E., Toombes G.E.S., Søgaard R., Koeppe II R.E., Gruner S.M., Hansen A.J., Andersen O.S. 2005. Capsaicin regulates voltage-dependent sodium channels by altering lipid bilayer elasticity. Mol. Pharmacol.68 (3): 680–689.
Nath B.S. 2003. Shifts in glycogen metabolism in hemolymph and fat body of the silkworm, Bombyx mori (Lepidoptera: Bombycidae) in response to organophosphorus insecticides toxicity. Pest. Biochem. Phys. 74: 73–84.
Olszewska J. 2010. Vanilloid receptors – comparison of structure and functions in mammals and invertebrates. Folia Biol. (Cracow) 58 (1–2): 1–7.
Olszewska J., Adamkiewicz B., Tęgowska E. 2010. Wpływ kapsazepiny na preferencje termiczne mącznika młynarka Tenebrio molitor. Mechanizmy służące utrzymaniu życia i regulacji fizjologicznych. [The effect of capsazepine on thermal preferences of mealworm Tenebrio molitor].XXIV Ogólnopolskie Seminarium (H. Lach, ed.). Cracow, 18.09.2010: 58–61.
Orhan I., Naz Q., Kartal M., Tosun F., Şener B., Choudhary I.M. 2007. In vitro anticholinesterase activity of various alkaloids. Z. Naturforsch. 62c: 684–688.
Scott I.M., Jensen H., Scott J.G., Isman M.B., Arnason J.T., Philogène B.J.R. 2003. Botanical insecticides for controlling agricultural pests: piperamides and the Colorado potato beetle Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Arch. Insect Biochem. Physiol. 54: 212–225.
Sosnowska D., Pruszyński S., Lipa J.J. 2009. Ewolucja metod i środków w zwalczaniu stonki ziemniaczanej (Leptinotarsa decemlineata Say). [Development of methods and insecticides for control of Colorado potato betele (Leptinotarsa decemlineata Say)]. Prog. Plant. Protection/Post. Ochr. Roślin 49 (2): 565–576.
Spitzer N.C., Lamborghini J.E. 1976. The development of the action potential mechanism of amphibian neurons isolated in culture. Proc. Natl. Acad. Sci. USA 73: 1641–1645.
Spurr E.B., McGregor P.G. 2003. Potential invertebrate antifeedants for toxic baits used for vertebrate pest control. Sci. Conservation 232: 1–36.
Stanković S., Zabel A., Kostic M., Manojlovic B., Rajkovic S. 2004. Colorado potato beetle.
Leptinotarsa decemlineata (Say) resistance to organophosphates and carbamates in Serbia. J. Pest. Sci. 77: 11–15. DOI: 10.1007/s10340-003-0020-7.
Szallasi A., Blumberg P.M. 1999. Vanilloid (capsaicin) receptors and mechanisms. Pharmacol. Rev. 51 (2): 160–211.
Tauber C.A., Tauber M.J., Gollands B., Wright R.J., Obrycki J.J. 1988. Preimaginal development and reproductive responses to temperature in two populations of the Colorado potato beetle (Coleoptera: Chrysomelidae). Ann. Entomol. Soc. Am. 81 (5): 755–763.
Tęgowska E., Grajpel B., Piechowicz B. 2005. Does red pepper contain an insecticidal compound for Colorado beetle? IOBC/WPRS Bull. 28 (10): 121–127.
Wawrzyniak M., Lamparski R. 2007. Ocena oddziaływania wyciągów z wybranych roślin zielarskich na żerowanie i rozwój stonki ziemniaczanej (Leptinotarsa decemlineata Say). [Evaluation of the effect of extracts from selected herbal plants on feeding and development of Colorado potato beetle (Leptinotarsa decemlineata Say)]. Prog. Plant Protection/Post. Ochr. Roślin 47 (4): 255–258.
Węgorek P. 2005. Current status of resistance in Colorado potato beetle (Leptinotarsa decemlineata Say) to selected active substances of insecticides in Poland. J. Plant Protection Res. 45 (4): 309–319.
Yu S.J. 2008. The Toxicology and Biochemistry of Insecticides. CRC Press, London, 276 pp.