Characterization of phytoplasmas related to aster yellows group infecting annual plants in Iran, based on the studies of 16S rRNA and rp genes
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Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, 84156, Isfahan, Iran
Leila Zirak
Division of Plant Pathology, Department of Plant Protection, College of Agriculture, Isfahan University of Technology, 84156, Isfahan, Iran
Submission date: 2013-10-12
Acceptance date: 2014-01-01
Journal of Plant Protection Research 2014;54(1):1–8
Several annual field crops, vegetables, ornamentals, oilseed crops, and weeds showing phytoplasma diseases symptoms were collected to detect phytoplasmas related to‘ Candidatus Phytoplasma asteris’. The collecting was done in the central regions of Iran. For general detection of phytoplasmas, 16S rRNA gene fragments were amplified using phytoplasma universal primer pair P1/P7 in polymerase chain reaction (PCR) followed by primer pair R16F2n/R16R2 in nested PCR. Then, for finer detection of phytoplasmas related to ‘Ca. P. asteris’, DNA samples were used to extend the rp and tuf gene fragments by PCR using aster yellows group specific primer pairs rp(I)F1A/rp(I)R1A and fTufAy/rTufAy, respectively. Restriction fragment lenght polymorphism (RFLP) analysis of rp gene fragments using digestion with Alu I, Mse I, and Tsp 509I restriction enzymes indicated that aster yellows group related phytoplasmas in these Iranian regions, belong to rp I-B subgroups. Sequence analysis of partial 16S rRNA and rp genes from representative phytoplasma isolates confirmed the RFLP results. This research is the first report of annual plants infected with phytoplasmas related to subgroup rp I-B in Iran.
The authors have declared that no conflict of interests exist.
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