ORIGINAL ARTICLE
 
KEYWORDS
TOPICS
ABSTRACT
Numerous plant species around the world suffer from the presence of viruses, which especially in economically important crops, cause irretrievable damage and/or extensive losses. Many biotechnological approaches have been developed, such as meristem culture, chemotherapy, thermotherapy or cryotherapy, to eliminate viruses from infected plants. These have been used alone or in combination. In this work, meristem culture, thermotherapy and cryotherapy were compared for Apple mosaic virus elimination from hazelnut local cultivar “Palaz”. The virus-free plant was also confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) after each treatment and, the best results were obtained by cryotherapy. A one step freezing technique, droplet vitrification, was used for cryotherapy, and the best regeneration percentage was 52%. After cryotherapy, virus-free seedlings of hazelnut local cultivar “Palaz” were confirmed as being virus-free after three subcultured periods.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
FUNDING
This study was supported by Mugla Sitki Kocman University, Scientific Research Projects Coordination Unit (Mugla, Turkey, MSKU-BAP, Project Number: 17-057).
 
REFERENCES (45)
1.
Akbas B., Degirmenci K. 2009. Incidence and natural spread of Apple mosaic virus on hazelnut in the west black sea coast of Turkey and its effect on yield. Journal of Plant Pathology 91 (3): 767–771. DOI: https://doi.org/10.4454/jpp.v9....
 
2.
Balamuralikrishnan M., Doraisamy S., Ganapathy T., Viswanathan R. 2002. Combined effect of chemotherapy and meristem culture on Sugarcane mosaic virus elimination in sugarcane. Sugar Tech 4 (2): 19–25. DOI: https://doi.org/10.1007/BF0295....
 
3.
Bettoni J.C., Costa M.D., Gardin J.P.P., Kretzschmar A.A., Pathirana R. 2016. Cryotherapy: a new technique to obtain grapevine plants free of viruses. Revista Brasileira de Fruticultura 38: 2–13. DOI: https://doi.org/10.1590/0100-2....
 
4.
Dĩaz-Barrita A.J., Norton M., Martĩnez-Peniche R.A., Uchanski M., Mulwa R., Skirvin R.M. 2008. The use of thermotherapy and in vitro meristem culture to produce virusfree ‘Chancellor’ grapevines. International Journal of Fruit Science 7 (3): 15–25. DOI: https://doi.org/10.1300/J492v0....
 
5.
Feng C., Wang R., Li J., Wang B., Yin Z., Cui Z., Li B., Bi W., Zhang Z., Li M., Wang Q. 2013. Production of pathogenfree horticultural crops by cryotherapy of in vitro-grown shoot tips. p. 463–482. In: “Protocols for Micropropagation of Selected Economically-Important Horticultural Plants” (M. Lambardi, E.A. Ozudogru, S.M. Jain, eds.). Methods in Molecular Biology, Clifton, New York, 490 pp. DOI: https://doi.org/10.1007/978-1-....
 
6.
Gergerich R.C., Dolja V.V. 2006. Introduction to plant viruses, the invisible foe. The Plant Health Instructor: 478. DOI: https://doi.org/10.1094/PHI-I-....
 
7.
Helliot B., Panis B., Poumay Y., Swennen R., Lepoivre P., Frison E. 2002. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Reports 20 (12): 1117–1122. DOI: https://doi.org/10.1007/s00299....
 
8.
Hu G., Dong Y., Zhang Z., Fan X., Ren F., Zhou J. 2015. Virus elimination from in vitro apple by thermotherapy combined with chemotherapy. Plant Cell, Tissue and Organ Culture 121 (2): 435–443. DOI: https://doi.org/10.1007/s11240....
 
9.
Hu J.S., Li H.P., Barry K., Wang M. 1995. Comparison of dot blot, ELISA, and RT-PCR assays for detection of two Cucumber mosaic virus isolates infecting banana in Hawaii. Plant Disease 79 (9): 902–906. DOI: https://doi.org/10.1094/PD-79-....
 
10.
Kaya E. 2015. Using reverse transcription-polymerase chain reaction (RT-PCR) for determination of Apple mosaic ilarvirus (ApMV) in hazelnut (Corylus avellana L.) cultivars. JSM Biochemistry and Molecular Biology 3 (1): 1011.
 
11.
Kaya E., Alves A., Rodrigues L., Jenderek M., Hernandez-Ellis M., Ozudogru A., Ellis D. 2013. Cryopreservation of Eucalyptus Genetic Resources. CryoLetters 34 (6): 608–618.
 
12.
Kaya E., Galatali S., Guldag S., Ozturk B. 2020. A new perspective on cryotherapy: pathogen elimination using plant shoot apical meristem via cryogenic techniques. p. 137–148. In: “Plant Stem Cells: Methods and Protocols” (M. Naseem, T. Dandekar, eds.). Springer, US, 150 pp. DOI: https://doi.org/10.1007/978-1-....
 
13.
Kaya E., Souza F.V.D. 2017. Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. In Vitro Cellular and Developmental Biology – Plant 53: 410–417. DOI: https://doi.org/10.1007/s11627....
 
14.
Kobylko T., Nowak B., Urban A. 2005. Incidence of Apple mosaic virus (ApMV) on hazelnut in south-east Poland. Folia Horticulturae 17 (2): 153–161.
 
15.
Kumar S., Khana M.S., Raja S.K., Sharmab A.K. 2009. Elimination of mixed infection of Cucumber mosaic and Tomato aspermy virus from Chrysanthemum morifolium Ramat. cv. Pooja by shoot meristem culture. Scientia Horticulturae 119 (2): 108–112. DOI: https://doi.org/10.1016/j.scie....
 
16.
Lambardi M., Sharma K.K., Thorpe T.A. 1993. Optimization of in vitro bud induction and plantlet formation from mature embryos of Aleppo pine (Pinus halepensis Mill.). In Vitro Cellular and Developmental Biology – Plant 29: 189–199. DOI: https://doi.org/10.1007/BF0263....
 
17.
Lloyd G., McCown B. 1980. Commercially feasible micropropagation of mountain laurel, Kalmia latifolia by use of shoot tip culture. International Plant Propagators’ Society 30: 421–427.
 
18.
López-Delgado H., Mora-Herrera M.E., Zavaleta-Mancera H.A., Cadena-Hinojosa M., Scott I.M. 2004. Salicylic acid enhances heat tolerance and potato virus X (PVX) elimination during thermotherapy of potato microplants. American Journal of Potato Research 81 (3): 171–176. DOI: https://doi.org/10.1007/BF0287....
 
19.
Marascuilo L.A., McSweeney M. 1977. Post-hoc multiple comparisons in sample preparations for test of homogeneity. p. 141–147. In: “Non-Parametric and Distribution-Free Methods for the Social Sciences” (M. McSweeney, L.A. Marascuilo, eds.). Pacific Grove, CA, USA: Brooks/Cole Publications.
 
20.
Menzel N., Jelkmann N., Maiss E. 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant m-RNA as internal control. Journal of Virological Methods 99: 89–92. DOI: https://doi.org/10.1016/S0166-....
 
21.
Milosevic S., Cingel A., Jevremovic S.B., Stankovic I., Bulajic A., Branka K., Subotic A. 2012. Virus elimination from ornamental plants using in vitro culture techniques. Journal Pesticides and Phytomedicine – Pesting 27 (3): 203–211. DOI: https://doi.org/10.2298/PIF120....
 
22.
Nukari A., Uosukainen M., Rokka V.M. 2009. Cryopreservation techniques and their application in vegetatively propagated crop plants in Finland. Agricultural and Food Science 18: 117–128. DOI: https://doi.org/10.2137/145960....
 
23.
O’Donnell K. 1999. Plant pathogen diagnostics: present status and future developments. Potato Research 42: 437–447. DOI: https://doi.org/10.1007/BF0235....
 
24.
Ozudogru E.A., Kaya E., Kirdok E., Issever-Ozturk S. 2011. In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cellular and Developmental Biology – Plant 47: 309–320. DOI: https://doi.org/10.1007/s11627....
 
25.
Paprstein F., Sedlak J., Polak J., Svobodova L., Hassan M., Bryxiova M. 2008. Results of in vitro thermotherapy of apple cultivars. Plant Cell Tissue and Organ Culture 94 (3): 347–352. DOI: https://doi.org/10.1007/s11240....
 
26.
Paprstein F., Sedlak J., Svobodova L., Polak J., Gadiou S. 2013. Results of in vitro chemotherapy of apple cv. Fragrance. Horticultural Science 40: 186–190. DOI: https://doi.org/10.17221/37/20....
 
27.
Ramgareeb S., Snyman S.J., van Antwerpen T., Rutherford R.S. 2010. Elimination of virus and rapid propagation of diseasefree sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture. Plant Cell Tissue and Organ Culture 100: 175–181. DOI: https://doi.org/10.1007/s11240....
 
28.
Rout G.R., Mohanpatra A., Jain M.S. 2006. Tissue culture of ornamental pot plant: A critical review on present scenario and future prospects. Biotechnology Advances 24 (6): 531–560. DOI: https://doi.org/10.1016/j.biot....
 
29.
Sakai A., Kobayashi S., Oiyama I. 1990. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports 9: 30–33. DOI: https://doi.org/10.1007/BF0023....
 
30.
Sellner L.N., Coelen R.J., Mackenzie J.S. 1992. A one-tube, one manipulation RT-PCR reaction for detection of Ross river virus. Journal of Virological Methods 40 (3): 255–263. DOI: https://doi.org/10.1016/0166-0....
 
31.
Slack S.A., Tufford L.A. 1995. Meristem culture for virus elimination. p. 117–128. In: “Plant Cell, Tissue and Organ Culture, Fundamental Methods” (O.L. Gamborg, G.C. Phillips, eds.), Springer-Verlag Berlin Heidelberg, 349 pp. DOI: https://doi.org/10.1007/978-3-....
 
32.
Spiegel S., Frison E.A., Converse R.H. 1993. Recent development in therapy and virus-detection procedures for international movements of clonal plant germplasm. Plant Disease 77: 176–1180. DOI: https://doi.org/10.1094/PD-77-....
 
33.
Spiegel S., Scott W., Bowman-Vance V., Tam Y., Galiakparov N.N., Rosner A. 1996. Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction. European Journal of Plant Pathology 102 (7): 681–685. DOI: https://doi.org/10.1007/BF0187....
 
34.
Tan R., Wang L., Hong N., Wang G. 2010. Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear. Plant Cell Tissue and Organ Culture 101: 229–235. DOI: https://doi.org/10.1007/s11240....
 
35.
Ustaoglu B., Karaca M. 2010. The possible effects of temperature conditions on hazelnut farming in Turkey. Itudergisi 9 (3): 153–161.
 
36.
Valasevich N., Cieślińska M., Kolbanova E. 2014. Molecular characterization of Apple mosaic virus isolates from apple and rose. European Journal of Plant Pathology 141: 839–845. DOI: https://doi.org/10.1007/s10658....
 
37.
Vivek M., Modgil M. 2018. Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’. Virus Disease 29 (1): 75–82. DOI: https://doi.org/10.1007/s13337....
 
38.
Wang Q.C., Cuellar W.J., Rajamäki M.L., Hiraka Y., Valkonen J.P.T. 2008. Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology 9: 237–250. DOI: https://doi.org/10.1111/j.1364....
 
39.
Wang Q., Liu Y., Xie Y., You M. 2006. Cryotherapy of potato shoot tips for efficient elimination of Potato leafroll virus (PLRV) and Potato virus Y (PVY). Potato Research 49: 119–129. DOI: https://doi.org/10.1007/s11540....
 
40.
Wang Q., Panis B., Engelmann F., Lambardi M., Valkonen J.P.T. 2009. Cryotherapy of shoot tips: a technique for pathogen elimination to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Annals of Applied Biology 154: 351–363. DOI: https://doi.org/10.1111/j.1744....
 
41.
Wang Q.C., Valkonen J.P.T. 2008a. Elimination of two viruses which interact synergistically from sweetpotato by shoot tip culture and cryotherapy. Journal of Virological Methods 154: 135–145. DOI: https://doi.org/10.1016/j.jvir....
 
42.
Wang Q.C., Valkonen J.P.T. 2008b. Efficient elimination of Sweetpotato little leaf phytoplasma fromsweetpotato by cryotherapy of shoot tips. Plant Pathology 57: 338–347. DOI: https://doi.org/10.1111/j.1365....
 
43.
Wang Q.C., Valkonen J.P.T. 2009. Cryotherapy of shoot tips: novel pathogen eradication method. Trends in Plant Science 14: 119–122. DOI: https://doi.org/10.1016/j.tpla....
 
44.
Wang B., Wang R.R., Cui Z.H., Bi W.L., Li J.W., Li B.Q., Ozudogru E.A., Volk G.M., Wang Q.C. 2014. Potencial applications of cryogenic technologies to plant genetic improvement and pathogen eradication. Biotechnology Advances 32: 583–595. DOI: https://doi.org/10.1016/j.biot....
 
45.
Ward E., Foster S.J., Fraaije B.A. McCartney H.A. 2004. Plant pathogen diagnostics: immunological and nucleic acidbased approaches. Annals of Applied Biology 145: 1–16. DOI: https://doi.org/10.1111/j.1744....
 
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