ORIGINAL ARTICLE
Compatibility of Metarhizium anisopliae (Ascomycota: Hypocreales) with several insectisides
 
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1
Plant Protection Department, Aboureihan Campus, University of Tehran, Iran
2
Iranian Research Institute of Plant Protection, Tehran, Iran
3
Department of Plant Protection, Campus of Agriculture and Natural Resources University of Tehran, Karaj, Iran 31587-11167., P.O.Box 4111
CORRESPONDING AUTHOR
Pourian Hamid-Reza
Department of Plant Protection, Campus of Agriculture and Natural Resources University of Tehran, Karaj, Iran 31587-11167., P.O.Box 4111
 
Journal of Plant Protection Research 2010;50(1):22–27
KEYWORDS
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ABSTRACT
The entomopathogenic fungus conidia viability and sporulation may be affected by different environmental factors or by bio-pesticides and chemical products used to protect cultivated plants. In this research, we investigated the compatibility of Metarhizium anisopliae DEMI 001 isolates with three insecticides: fipronil, pyriproxyfen and hexaflumuron. The effect of fipronil, pyriproxyfen and hexaflumuron on sporulation, vegetative growth and conidial germination of the fungus was studied based on measuring the vegetative growth and sporulation. SDA medium was mixed with fipronil (10, 40 and 120 ppm), pyriproxyfen (10, 500 and 1 000 ppm), hexaflumuron (20, 50 and 120 ppm). SAD medium excluding insecticides was designated as blank. To determine the sporulation rate, spores was counted on surface area and vegetative growth was measured on the basis of colony diameter. The results showed that hexaflumuron at the concentration of 120 ppm reduced the vegetative growth to 0% showing the highest reduction effect compared to pyriproxyfen (24.59%) and fipronil (24.31%). All three insecticides reduced drastically the conidial germination at the highest concentrations (0–15%). Hexaflumuron treatment with 0% germination at all three concentrations and fipronil and pyriproxyfen with 32.36% and 9.7% germination at 200 ppm were not significantly different. Regarding the highest negative effect of hexaflumuron on germination at all three concentrations, this insecticide should not be applied together with the entomopathogenic fungus.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (33)
1.
Alizadeh A., Mohamad-Amin S., Masood Kh., Saberi-Riseh R. 2007. Compatibility of Beauveria bassiana (Bals.) Vuill. with several pesticides. Int. J. Agri. Bio. 9 (1): 31–34.
 
2.
Alves S.B., Lecuona R.E. 1998. Epizootiologia aplicada ao controle microbiano de insetos. p. 97–170. In: “Controle Microbiano de Insetos” (S.B. Alves, ed.). São Paulo, Fealq, 1163 pp.
 
3.
Alves S.B., Monino Jr A., Almeida J.E.M. 1998. Produtos fitossanitarios e entomopatogênicos. p. 289–370. In: “Controle Microbiano de Insetos” (S.B. Alves, ed.). São Paulo, Fealq, 1163 pp.
 
4.
Anderson T.E, Roberts D.W. 1983. Compatibility of Beauveria bassiana isolate with insecticide formulations used in Colorado Potato Beetle (Coleotera: Crysomelidae) Control. J. Econ. Entomol. 76: 1437–1441.
 
5.
Ansari M.A., Shah F.A., Whittaker M., Prasad M., Butt T.M. 2007. Control of western flower thrips, Frankliniella occidentalis pupae with Metarhizium anisopliae in peat and peat alternative growing media. Biol. Control 40: 93–297.
 
6.
Azaizeh H., Gindin G., Said O., Barash I. 2002. Biological control of the western flower thrips, Frankliniella occidentalis in cucumber using the entomopathogenic fungus Metarhizium anisopliae. Phytoparasitica 30: 1–7.
 
7.
Batista Filho A., Aves S.B. 1998. Formulacao de entomopatogênicos. p. 917–66. In: “Controle Microbiano de Insetos” (S.B. Alves, ed.). São Paulo, Fealq, 1 163 pp.
 
8.
Batista Filho A., Leite L.G. 1995. Enhanced activity of Beauveria bassiana (Bals.) Vuill. associated with mineral oil against Cosmopolites sordidus (Germar) adults. Ann. Soc. Entomol. Brasil 24: 405–408.
 
9.
Behle R., Compton D., Laszlo J., Shapiro Ilan D. 2006. Potential use of soyscreen in formulations of Beauveria bassiana for UV protection. Entomol. Soci. Amer. Ann. Meet. North Central Branch. Paper No. D-215.
 
10.
Benz G. 1987. Environment. p. 177–214. In: “Epizootiology of Insect Diseases” (R . Fuxa, Y. Tanada, eds.). New York, Wiley, 960 pp.
 
11.
Boucias D.G., Pendland J.C. 1988. Nonspecific factors involved in attachment of entomopathogenic deuteromycetes to host insect cuticle. Appl. Environ. Microbiol. 54: 1795–1805.
 
12.
Butt T.M., Jackson C., Magan N. 2001. Introduced-fungal biocontrol agent: progress, problems and potential. p. 1–8. In: “Fungi as Biocontrol Agent” (T.M. Butt, C. Jackson, N. Magan, eds.). CABI International, UK, 390 pp.
 
13.
De Olivera R.C., Neves P.M.O.J. 2004. Biological control compatibility of Beauveria bassiana with acaricides. Neotrop. Entomol. 33: 353–358.
 
14.
Duarte A., Menendez J.M., Triguero N. 1992. Estudio preliminar sobre la compatibilidad de Metarhizium anisopliae com algunos plaguicidas quimicos. Rev. Baracoa 22: 31–39.
 
15.
Ekesi S., Maniania N.K. 2000. Susceptibility of Megalurothrips sjostedti development stages to Metarhizium anisopliae and the effects of infection on feeding adult fecundity, egg fertility and longevity. Entomol. Exp. Appl. 94: 229–236.
 
16.
Furlong M.J., Pell J.K. 1997. The influence of environmental factors on the persistence of Zoophthora radicans conidia. J. Inverteb. Pathol. 69: 223–233.
 
17.
Ghini R., Kimati H. 2000. Resistência de fungos a fungicidas. Jaguariuna, EMBRAPA Meio Ambient, 78 pp.
 
18.
Kimati H. 1995. Controle químico. p. 761–785. In: “Manual de Fitopatologia” (A. Bergamin Filho, H. Kimati, L. Amorin, eds.). São Paulo, Agronomica Ceres, 919 pp.
 
19.
Li D.P., Holdom D.G. 1995. Effects of nutrients on colony formation, growth, and sporulation of Metarhizium anisopliae (Deuteromycotina, Hyphomycetes). J. Invertebr. Pathol. 65: 253–260.
 
20.
Loria R., Galaini S., Roberts D.W. 1983. Survival of inoculum of the entomopathogenic fungus Beauveria bassiana as influenced by fungicides. Environ. Entomol. 12: 1724–1726.
 
21.
Malo A.R. 1993. Estudio sobre la compatibilidad del hongo Beauveria bassiana (Bals.) Vuill. con formulaciones comerciales de fungicidas e insecticidas. Rev. Colomb. Entomol. 19: 151–158.
 
22.
Maniania N.K., Ekesi S., Lohr B., Wangi F. 2003. Prospects for biological control of the flower thrips, Frankliniella occidentalis, with the entomopathogenic fungus, Metarhizium anisopliae on chrysanthemum. Mycopathologia 155: 229–235.
 
23.
Moino Jr A., Alves S.B. 1998. Efeito de imidacloprid e fipronil sobre Beauveria bassiana (Bals.) Vuill. e Metarhizium anisopliae (Metsch.) Sorok. no comportamento de limpeza de Heterotermes tenuis (Hagen). Ann. Soc. Entomol. Brasil 27: 611–619.
 
24.
Neves P.M.O.J., Hirose E., Tchujo P.T. 2001. Compatibility of entomopathogenic fungi with neonicotinoid insecticides. Neotrop. Entomol. 30: 263–268.
 
25.
Osborne L.S., Boucias D.G. 1985. A review of chemical antagonists to mycopathogens of citrus root weevils. Fla. Entomol. 68: 409–416.
 
26.
Quintela E.D., McCoy C.W. 1998. Synergistic effect of imidacloprid and two entomopathogenic fungi on the behavior and survival of larvae of Diaprepes abbreviatus (Coleoptera: Curculionidae) in soil. J. Econ. Entomol. 91: 110–122.
 
27.
Roberts D.W., Campbell A.S. 1977. Stability of entomopathogenic fungi. Misc. Publ. Entomol. Soc. Am. 10: 19–75.
 
28.
St. Leger R.J., Cooper R.M. 1987. Production of cuticle-degrading enzymes by the Metarhizium anisopliae during infection of cuticles from Calliphora vomitoria and Manduca sexta. J. Gen. Microbiol. 133: 1371–1382.
 
29.
St. Leger R.J., Goettel M., Roberts D.W. 1991. Preparation events during infection of host cuticle by Metarhizium anisopliae. J. Invertebr. Pathol. 58: 168–179.
 
30.
SYSTAT 2005. Chicago, IL: SPSS Science Marketing Departnt SPSS Inc.
 
31.
Todorova S.I., Coderre D., Duchesne R.M. 1998. Compatibility of Beauveria bassiana with selected fungicides and herbicides. Environ. Entomol. 27: 427–433.
 
32.
Vestergarrd S., Gilliespie A.M., Butt T.M., Schreiter G., Eilenberg J. 1995. Pathogenicity of the hyphomycetes fungi Verticillium lecanii and Metarhizium anisopliae to the western flower thrips, Frankliniella occidentalis. Biocontrol Sci. Technol. 5: 185–192.
 
33.
Zimmerman G. 1975. Über die Wirkung systemischer Fungizide auf verschiedene insektenpathogene Fungi imperfecti in vitro. Nachrichtenbl. Dtsch. Pflanzenschutzdienst (Berl.) 27: 113–117.
 
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