ORIGINAL ARTICLE
Correlation between bean seed surface lipids and Acanthoscelides obtectus Say development
 
More details
Hide details
1
University of Warmia and Mazury, Department of Phytopathology and Entomology Prawocheńskiego 17, 10-722 Olsztyn, Poland
2
University of Gdańsk, Department of Chemistry Sobieskiego 18, 80-952 Gdańsk, Poland
3
University of Warmia and Mazury, Department of Plant Food Chemistry and Processing
CORRESPONDING AUTHOR
Mariusz Nietupski
University of Warmia and Mazury, Department of Phytopathology and Entomology Prawocheńskiego 17, 10-722 Olsztyn, Poland
 
Journal of Plant Protection Research 2005;45(2):125–133
KEYWORDS
TOPICS
ABSTRACT
The influence of bean seed surface lipids on infestation of seeds by Acanthoscelides obtectus Say was investigated. The experiments were performed in dual-choice bioassays on three bean varieties: Blanka, Bor and Longina. The collected data for natural and solvent washed seeds concerned the number of ovipositions, embryo mortality, lack of seed-boring activity, dead larvae inside seeds and developed insects. The results clearly indicated that bean seed surface lipids are involved in all infestation stages, and could be used to distinguish resistant and non-resistant varieties of been. Chemical analyses revealed the following groups of surface lipids: wax esters, long chain primary alcohols, n-alkanes, sterols, fatty acids, squalene, aldehydes, monoacylglycerols, ketones and fatty acid esters. Quantitative composition of surface lipids was analysed using selected chemometric procedures to determine correlation with bioactivity. Cluster analysis of surface lipid composition enabled to distinguish resistant and non-resistant varieties. Fatty acids and monoacylglycerols were found to deter bean weevil infestation, while alkan-1-ols acted as attractants.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (18)
1.
Brzostek G., Ignatowicz S. 1992. Oviposition preference of the bean weevil, Acanthoscelides obtectus Say (Coleoptera: Bruchidae), for varieties of the common bean. Ann. Warsaw Agricult. Univ. – SGGW, Horticult., 16: 65–77.
 
2.
Ciepielewska D., Fornal Ł. 1993. The preference of the bean (Acanthoscelides obtectus Say, Coleoptera, Bruchidae). Part II. Morphological and physiological characters of bean seeds of various cultivars and the development of the insect pest. Pol. Pismo Entomol., 62: 153–160.
 
3.
Eigenbrode S. D., Espelie K. E. 1995. Effects of plant epicuticular lipids on insect herbivores. Ann. Rev. Entomol., 40: 171–194.
 
4.
Espelie K.E., Bernays E.A., Brown J.J. 1991. Plant and insect cuticular lipids serve as behavioural cues for insects. Arch. Insect Biochem. Physiol., 17: 223–233.
 
5.
Fornal Ł., Ciepielewska D., Pierzynowska-Korniak G. 1993. The preference of the bean (Acanthoscelides obtectus Say, Coleoptera, Bruchidae). Part III. The effect of carbohydrate and tannin fractions. Pol. Pismo Entomol., 62: 161–167.
 
6.
Hedin P. A. (ed.). 1983. Plant resistance to insects. ACS Symposium Series 208,Washington, pp. 540.
 
7.
Mardia K.V., Kent J.T., Bibby J.M. 1979. Multivariate Analysis. Academic Press. N.Y.: 110–130.
 
8.
Massart D.L., Kaufman L. 1983. Hierarchical clustering methods. The interpretation of analytical data by use of cluster analysis.Wiley, N.Y.: 76–92.
 
9.
Mirkov T.E.,Wahlstrom J., Hagiwara K., Finardi-Filho F., Kjemtrup S., Chrispeels M.J. 1994. Evolutionary relationships among proteins in the phytohemagglutinin-arcelin-a-amylase inhibitor family of the common bean and its relatives. Plant Mol. Biology 26: 1103–1113.
 
10.
Nietupski M., Ciepielewska D., Szafranek B., Synak E., Szafranek J., Maliński E., Konopka I., Fornal Ł. 2001. Bean seed surface lipids – a variety trait which inhibits the development of bean weevil, Proc. 4th European Conference on grain legumes. Cracow, Poland, 8–12 July 2001, p. 285.
 
11.
Otto M. 1999. Chemometrics. Wiley–VCH, Weinheim: 119–158.
 
12.
Städler E. 1986. Oviposition and feeding stimulation in leaf surface waxes. p. 105–121. In “Insect and the Plant Surface” (B. E. Juniper, T.R.E. Southwood, eds.). Edward Arnold, London.
 
13.
Stamopoulos D., Huignard J. 1980. L’influence des diverses parties de la graine de haricot (Phaseolus vulgaris) sur le développement des larves d’Acanthoscelides obtectus (Coléoptère Bruchidae). Entomol. Exp. Appl., 28: 38–46.
 
14.
Szafranek J., Szafranek B. 2001. Substancje powierzchniowe roślin i ich rola w oddziaływaniach roślina-owad. pp. 231–249. In „Biochemiczne Oddziaływania Środowiskowe” (W. Oleszek, K. Głowniak, B. Leszczyński, eds.). Wydawnictwo Akademii Medycznej w Lublinie.
 
15.
Thiéry D. 1984. Hardness of some fabaceous seed coats in relation to larval penetration by Acanthoscelides obtectus (Say) (Coléoptera:Bruchidae). J. Stored Prod. Res., 20: 177–181.
 
16.
Thièry D. 1982. Influence de lateneur en eau et de ladureté de teguments de graines de Phaseolus vulgaris sur la fréquence de pénétration des larves néonates d’Acanthoscelides obtectus. Entomol. Exp. Appl., 32: 141–145.
 
17.
Thiéry D., Jarry M., Pouzat J. 1994. To penetrate or not penetrate? A behavioural choice by bean beetle first-instars larvae in response to Phaseolus vulgaris seed surface quality. J. Chem. Ecology 20: 1867–1875.
 
18.
Winiecki Z., Szafranek J., Maliński E., Nawrot J. 1994. Wpływ wosków powierzchniowych ziarna pszenicy na żerowanie szkodników magazynowych. Materiały 34. Sesji Nauk. Inst. Ochr. Roślin cz. 2: 28–32.
 
eISSN:1899-007X
ISSN:1427-4345