ORIGINAL ARTICLE
Pathogenicity of selected isolates of the quarantine pinewood nematode Bursaphelenchus xylophilus to Scots pine (Pinus sylvestris L.)
 
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Department of Biological Pest Control, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, Poland
CORRESPONDING AUTHOR
Anna Filipiak
Department of Biological Pest Control, Institute of Plant Protection – National Research Institute, Władysława Węgorka 20, 60-318 Poznań, Poland
Submission date: 2015-06-09
Acceptance date: 2015-10-23
 
Journal of Plant Protection Research 2015;55(4):378–382
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TOPICS
ABSTRACT
The pinewood nematode (PWN), Bursaphelenchus xylophilus , is the causal agent of pine wilt disease (PWD). This nematode is considered to be an indigenous to North America and was introduced to Japan in the late 19th century. Subsequently, it has spread throughout Japan and in many other countries, China, Taiwan, and South Korea. In 1999, B. xylophilus was discovered in Portugal, and in 2008 in Spain. So far the studies have revealed that the pathogenicity of B. xylophilus varies between different isolates. The conducted study compared the pathogenicity of five isolates of B. xylophilus , originating from different parts of Japan, to 3-year-old Pinus sylvestris, and their ability to reproduce in the seedlings. The results revealed diverse virulence of B. xylophilus resulting in plant mortality. Three isolates S10, Ka4, and T4 caused 100% mortality of plants within three months while at the same time, the other two isolates, C14-5 and OKD-1 did not cause any disease symptoms on plants. After seven months, some dieback occurred on two seedlings, but similar symptoms were also found on the control plant. Moreover, a significant positive correlation was found between nematode virulence and the number of nematodes reproducing on pine seedlings.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (27)
1.
Aikawa T., Kikuchi T., Kosaka H. 2006. Population structure of Bursaphelenchus xylophilus within single Pinus thunbergii trees inoculated with two nematode isolates. Forest Pathology 36 (1): 1–13.
 
2.
Aikawa T., Kikuchi T. 2007. Estimation of virulence of Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae) based on its reproductive ability. Nematology 9 (3): 371–377.
 
3.
Braasch H. 2000. Influence of temperature and water supply on mortality of 3-year-old pines inoculated with Bursaphelenchus xylophilus and B. mucronatus. Nachrichtenblatt des Deutschen Pflanzenschutzdienstes 52 (10): 244–249.
 
4.
Caroppo S., Cavalli M., Coniglio D., Ambrogioni L. 2000. Pathogenicity studies with various Bursaphelenchus populations on conifer seedlings under controlled and open air conditions. Redia 83: 61–75.
 
5.
Dropkin V.H., Foudin A., Kondo E., Linit M., Smith M., Robbins K. 1981. Pinewood nematode: a threat to U.S. forests? Plant Disease 65: 1022–1027.
 
6.
EPPO 2015. Prepared by CABI and EPPO for the EU under Contract 90/399003. Available on: http://www.cabi.org/isc/datash... [Accessed: April 22, 2015].
 
7.
Espada M., Silva A.C., Akker S.E., Cock P.J.A., Mota M., Jones J.T. 2015. Identification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multilayered detoxification strategy. Molecular Plant Pathology. DOI: 10.1111/mpp.12280.
 
8.
Filipiak A. 2008. The pine wilt disease. Sylwan 12: 9–19.Furuno T., Nakai I., Uenaka K., Haya K. 1993. Pine wilt disease of introduced pine species planted in Kamigamo and Shirahama Experimental Stations of Kyoto University Forest – resistance of Pinus spp. to the pine wood nematode. The Reports of the Kyoto University Forests 25: 20–34. (in Japanese).
 
9.
Futai K., Furuno T. 1979. The variety of resistances among pine species to pine wood nematode, Bursaphelenchus lignicolous. Bulletin of the Kyoto University Forests 51: 23–26.
 
10.
GUS – Główny Urząd Statystyczny 2012. Leśnictwo. Informacje i opracowania statystyczne. Warszawa, 341 ss.
 
11.
Ikeda T., Suzuki T. 1984. Influence of pine-wood nematodes on hydraulic conductivity and water status in Pinus thunbergii. Journal of the Japanese Forestry Society 66 (10): 412–420. (in Japanese, with English summary).
 
12.
Jiao G., Shen P., Li H.1996. A study on the pathogenicity of pine wood nematodes from Japan and Nanjing, China to Cedrus deodara and Pinus massoniana. Plant Quarantine 10 (4): 193–195. (in Chinese, with English summary).
 
13.
Kiyohara T., Tokushige Y. 1971. Inoculation experiments of a nematode, Bursaphelenchus sp., onto pine trees. Journal of the Japanese Forestry Society 53: 210–218. (in Japanese, with English summary).
 
14.
Kiyohara T., Bolla R.I. 1990. Pathogenic variability among populations of the pinewood nematode, Bursaphelenchus xylophilus. Forest Science 36 (4): 1061–1076.
 
15.
Kojima K., Kamijyo A., Masumori M., Sasaki S. 1994. Cellulase activities of pine-wood nematode Bursaphelenchus xylophilus isolates with different virulences. Journal of the Japanese Forestry Society 76: 258–262. (in Japanese, with English summary).
 
16.
Kozłowski M.W. 2003. Native and exotic sawyer beetles, Monochamus spp. (Coleoptera, Cerambycidae) as the vectors of the pine wilt nematode, Bursaphelenchus xylophilus. Sylwan 1: 24–34.
 
17.
Li H. 2008. Identification and pathogenicity of Bursaphelenchus species (Nematoda: Parasitaphelenchidae). Ph.D. thesis, Ghent University, Ghent, Belgium, 223 pp.
 
18.
Mota M.M., Braasch H., Bravo M.A., Penas A.C., Burgermeister W., Metge K., Sousa E. 1999. First report of Bursaphelenchus xylophilus in Portugal and in Europe. Nematology 1 (7–8): 727–734.
 
19.
Mota M., Takemoto S., Takeuchi Y., Hara N., Futai K. 2006. Comparative studies between Portuguese and Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus. Journal of Nematology 38 (4): 429–433.
 
20.
Robertson L., Cobacho Arcos S., Escuer M., Santiago Merino R., Esparrago G., Abelleira A., Navas A. 2011. Incidence of the pinewood nematode Bursaphelenchus xylophilus Steiner & Buhrer, 1934 (Nickle, 1970) in Spain. Nematology 13 (6): 755–757.
 
21.
Rutherford T.A., Webster J.M. 1987. Distribution of pine wilt disease with respect to temperature in North America, Japan and Europe. Canadian Journal of Forest Research 17 (9): 1050–1059.
 
22.
Rutherford T.A., Mamiya Y., Webster J.M. 1990. Nematode-induced pine wilt disease: factors influencing its occurrence and distribution. Forest Science 36 (1): 145–155.
 
23.
Shinya R., Morisaka H., Kikuchi T., Takeuchi Y., Ueda M., Futai K. 2013. Secretome analysis of the pine wood nematode Bursaphelenchus xylophilus reveals the tangled roots of parasitism and its potential for molecular mimicry. PLoS One 8 (6): e67377.
 
24.
Takemoto S., Kanzaki N., Futai K. 2005. PCR-RFLP image analysis: a practical method for estimating isolate-specific allele frequency in a population consisting of two different strains of the pinewood nematode, Bursaphelenchus xylophilus(Aphelenchida: Aphelencoididae). Applied Entomology and Zoology (Jpn) 40: 529–535.
 
25.
Togashi K., Shigesada N. 2006. Spread of the pinewood nematode vectored by the Japanese pine sawyer: modeling and analytical approaches. Population Ecolology 48 (4): 271–283.
 
26.
Tomalak M. 2004. Ocena przydatności dostępnych metod do identyfikacji taksonomicznej kwarantannowego nicienia – węgorka sosnowca [Bursaphelenchus xylophilus (Steiner et Bührer) Nickle]. [Evaluation of methods available for taxonomic identification of the Bursaphelenchus xylophilus]. Progress in Plant Protection/Postępy w Ochronie Roślin 44 (1): 452–461.
 
27.
Wang Y., Yamada T., Sakaue D., Suzuki K. 2005. Variations on the life history parameters and their influence on rate of population increase of different pathogenic isolates of the pine wood nematode, Bursaphelenchus xylophilus. Nematology 7 (3): 459–467.
 
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