Dual effects of leaf extracts of Eucalyptus citriodora on controlling purslane and root-knot nematode in sunflower
More details
Hide details
Botany Department, National Research Centre, Dokki, P.O. Code 12622 Cairo, Egypt
Plant Pathology Department, Nematology Laboratory, National Research Centre Dokki, P.O. Code 12622 Cairo, Egypt
Corresponding author
Kowthar Gad El-Rokiek
Botany Department, National Research Centre, Dokki, P.O. Code 12622 Cairo, Egypt
Journal of Plant Protection Research 2011;51(2):121-129
Experiments were conducted under laboratory and greenhouse conditions to investigate the allelopathic activity of aqueous extracts of dry and fresh leaves of Eucalyptus citriodora on purslane weed growth and nematode Meloidogyne incognita infecting sun- flower plants cv. Giza 102. A Petri dish biotest showed that the aqueous extracts significantly reduced purslane ( Portulaca oleracea L.) seedling length, with the degree of inhibition being dependent on the extract concentration. The fresh and dry leaf extracts of E. citriodora standard solution “S” caused the highest net mortality percentage of 100% after 72 hrs of exposure. Greenhouse studies in 2008 and 2009, indicated the greatest significant inhibition in purslane growth as well as the number of galls and egg masses of infecting nematode affected the increase in sunflower growth and yield. The studies indicated increase in the endogenous contents of total phenols in purslane tissues. This increase, correlated with growth inhibition due to treatment with leaf extract of E. citriodora . Chemical analysis indicated an increase in the contents of carbohydrates, protein and oil in sunflower seeds. The analysis of fatty acid composition by Gas Liquid Chromatography (GLC) indicated increases in the percentage of oleic and linoleic acid in sunflower seeds when fresh leaf extract of E. citriodora was used. A high-performance liquid chromatography analysis showed that the following acids; caffeic, ferulic, coumaric, benzoic, vanelic, chlorogenic, and hydroxybenzoic were present in Eucalyptus extracts.
The authors have declared that no conflict of interests exist.
Al-Naib F.A.G., Al-Mousawi A.H. 1976. Allelopathic effects of Eucalyptus microtheca. Identification and characterization on the phenolic compounds in Eucalyptus microtheca. J. Univ. Kuwit (Sci.) 3: 83–87.
Alonso-Prados J.L., Hernandez-Sevillano E., Llanos S., Villarroya M., Garcia- Baudin J. M. 2002. Effects of sulfosulfuron soil residues on barley (Hordeum vulgare), sunflower (Helianthus annuus) and common vetch (Vicia sativa). Crop Protect. 21: 1061–1066.
Aly R., Goldwasser Y., Eizenberg H., Hershenhorn J., Golan S., Kleifeld Y. 2001. Broomrape (Orobanche cumana) control in sunflower (Helianthus annuus) with imazapic. Weed Technol. 15 (2): 306–309.
AOCS. 1964. Official and Tentative Methods of American Oil Chemists Society and American Chem. 3rd ed. Vols. I and II, Ac 3-44. Champaign, IL.
Cheema Z.A, Khaliq A., Mubeen M. 2003. Response of wheat and winter weeds to foliar application of different plant water extracts of sorghum (S. bicolor).Pak. J. Weed Sci. Res. 9 (1–2): 89–97.
Chon S.U., Kim Y.M. 2004.Herbicidal potential and quantification of suspected allelo-chemicals from four grass Crop Extracts J. Agron. Crop Sci. 190 (2): 145–150.
Chon S.U., Kim Y.M., Lee J.C. 2003. Herbicidal potential and quantification of causative- allelochemicals from several Compositae weeds.Weed Res. 43 (6): 444–448.
Davis R.F., Webster T.M. 2005.Relative host status of selected weeds and crops for Meloidogyne incognito and Rotylenchulus reniformis. J. Cotton Sci. 9: 41–46.
El-Hamshary O.I.M., Wafaa M.A. El-Nagdi, Youssef M.M.A. 2006. Genetical studies and antagonistic effects of a newly bacterial fusant against Meloidogyne incognita, root-knot nematode, infecting sunflower and plant pathogen Futatcum elsporum. Pak. J. Biotech. 3 (1–2): 61–70.
El-Nagdi W.M.A. 2005. Comparative efficacy of aqueous jojoba dry leaves extract and jojoba&castor oil commercial product on the root-knot, Meloidogyne incognita infecting pepper plant. Egypt. J. Agric. Res. NRC 2 (1):425–437.
El-Rokiek Kowthar G. 2007. Evaluating the physiological influence of benzoic and cinnamic acids, alone or in combination on wheat and some infested weeds comparing with the herbicide isoproturon. Ann. Agric. Sci., Ain Shams Univ., Cairo 52 (1): 45–58.
El-Rokiek Kowthar G., Aid Rawia A. 2009. Allelopathic effects of Eucalyptus citriodora on amaryllis and associated grassy weed. Planta Daninha 27 (Numero Especial):887– 899.
El-Rokiek Kowthar G., El-Shahawy T.A., Sharara F.A. 2006. New approach to use rice straw waste for weed control II. The effect of rice straw extract and fusilade (herbicide) on some weeds infesting soybean (Gllecomae L.). Int. J. Agric. Biol. 8 (2): 269–275.
Harborne J.B. 1984. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 2nd ed., Phytochem. Methods. Chapman and Hall, New York, 3: 100–117.
Herbert D., Phipps P.J., Strange R.E. 1971. Determination of total carbohydrate. Methods Microbiol. 5B: 209–344.
Hussein H.F. 2001. Estimation of critical period of crop-weed competition and nutrient removal by weeds in onion (Allium cepa,L.) in sandy soil. Egypt. J. Agron. 24 (1): 43–62.
Inderjit I. 2002. Multifaceted approach to study allelochemicals in an ecosystem. p. 231–256. In: “Allelopathy, from Molecules to Ecosystems” (M.J. Reigosa, N. Pedrol, eds.). Science Publishers, Enfield, New Hampshire, 641 pp.
Khan A., Sayed M., Shaukat S.S., Handoo Z.A. 2008. Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan. Nematol. Medit. 36 (1): 93–98.
Koenning S.R., Overstreet C., Noling J. W., Donald P.A., Becker J.O., Fortnum B.A. 1999. Survey of crop losses in response to phytoparasitic nematodes in the United States for 1994. J. Nematol. (Suppl.) 31 (4): 587–618.
Korayem A.M., Wahab A., Mohamed M.M. 2006. Management of the root-knot nematode, Meloi dogyne incognita on sunflower by a formulated soil amendment.Al-Azhar J. Agric. Res. 3 (2): 263–268.
Lowery O.H., Rosebrough N.J., Farr A.L., Randall R.J. 1951. Protein measurements with folin phenol reagent. J. Biol. Chem. 193 (1): 265–275.
Lu Y.H. 1999. Syntheses of Analogs of Ageratochromene and Their Inhibitory Effects on Plants Micro-Organisms. MS thesis, South China Agric. Univ., Guangzhou.
Luo S.M., Lin X.L, Zeng R.S., Kong C.H., Cao P.R. 1995. Allelopathy of trop. Plants in agro-ecosystem of South China. Eco. Sci. 2 (2): 114–128.
Marshall E.J.P., Brown V.K., Boatman N.D., Lutman P.J.W., Squire G.R., Ward L.K. 2003. The role of weeds in supporting biological diversity within crop fields. Weed Res. 43 (2): 77–89.
May F.E, Ash J.E. 1990. An assessment of the allelopathic potential of Eucalyptus. Aust. J. Bot. 38 (3): 245–254.
Mohamed M.M.M. 2005. Studies on Nematode Pests Associated with Some Oil Crops and Their Control Measures. Ph.D. Theisis, Fac. Agric., Cairo Univ., 182 pp.
Montogomery R. 1961. Further studies of the phenol-sulphuric acid reagent for carbohydrate. Biochem. Bioph. Acta 48: 591–593.
Nemat Alla M.M., Younis M.E. 1995. Herbicide effects on phenolic metabolism in miaze (Zea mays L.) and Soybean (Glycine Mae L.) seedlings. J. Exp. Bot. 46 (292): 1731–1736.
Ramappa H.K, Muniyappa V., Colvin J. 1998. The contribution of tomato and alternative host plants to tomato leaf curl virus inoculum pressure in different areas of south India. Ann. Appl. Biol. 133 (2): 187–198.
Sanchez J.D., Expósito M.J., Granados F.L., Muñoz M.C., García-Torres L. 2003. pronamide applied to sunflower seeds for Orobanche Cumana. Control Weed Tech. 17 (2): 314–319.
Shahidi F. 1999.Canola and Rapeseed. Van Nostrand Reinhold, New York, USA, 93 pp.
Singh H.P., Batish D.R., Kaur S., Kohli R.K. 2003. Phytotoxic interference of Ageratum onyzoides with wheat (Triticum aestivum). J. Agron. Crop Sci. 189 (5): 341–346.
Singh H.P., Batish D.R., Setia N., Kohli R.K. 2005. Herbicidal activity of volatile oils from Eucalyptus citriodora against Parthenium hysterophorus.Ann. Appl. Biol. 146 (1): 89–94.
Snedecor G.W., Cochran W.G. 1980. Statistical Methods. 7th ed. The Iowa State Univ. Press, Ames, Iowa, I A., 507 pp.
Snell F.D, Snell C.T. 1953. Colorimetric Methods. Volume III. Organic, D. Van Nostrand Company, Inc. Toronto, New York, London, 606 pp.
Stephanie R.D., Anita D.J., Eric L.B., David L.R., Scott A.S. 2004. Common sunflower (Helianthus annuus) and shattercane Sorghum bicolor) interference in corn. Weed Sci. 52 (6): 976–983.
Venkatesh R., Harrison S.K., Riedel R.M. 2000. Weed hosts of soybean cyst nematode Heterodera glycines) in Ohio. Weed Tech. 14 (1): 156–160.
Webster T.M. 2004. Southern states weed survey: grass crops subsection. Proc. Southern Weed Sci. Soc. 57: 404–426.
Weston L.A.1996.Utilization of allelopathy for weed management in agroecosytems. Agron. J. 88 (6): 860–866.
Youssef M.M.A., Korayem A.M., Mohamed M.M. 2008.Effect of certain organic amendment extracts and furfural on M. incognita root-knot nematode infesting sunflower. 5th Congress of Scientific Research Outlook in the Arab World., Fez, Morocco, 25–30 October 2008, p. 129.
Zhang M., Ling B., Kong C.H., Zhao H., Pang X. 2002. Allelopathic potential of volatile oil from Mikania micrantha. Chinese J. Appl. Eco.13: 1300–1302.
Journals System - logo
Scroll to top