• The EEOL significantly reduced infection rates, decreased root necrosis indices, and promoted increased plant height, stem diameter, and leaf area in both models.
  • The EEOL also lowered the percentage of fallen trees and enhanced harvest yields in the field model.
  • At a dosage of 96.54 l•ha-1, EEOL exhibited effectiveness equivalent to the conventional chemical nematode control method, fenamipos.
Eupatorium odoratum is known for its ability to resist nematode infestations that attack the root systems of banana plants. An aqueous extract of the leaves and stems of E. odoratum (named EEOL) represents a natural solution that we investigated for its potential to control the harmful nematode, Radopholus similis, in Cavendish banana plants. Our research into EEOL’s efficacy spanned two distinct environments: a micro plot experiment model and a field model. Various concentrations of EEOL were examined to assess its efficacy in alleviating R. similis infestations and in mitigating their adverse effects on Cavendish banana plants. In the micro plot experiment model, the concentration of the original solution, diluted at ratios of 1:30×, 1 : 16×, 1 : 8×, 1 : 4×, and 1 : 2×, ranged from 1.76 to 28.16 mg · ml-¹. In the field model, the corresponding rates varied from 6.03 to 96.54 l · ha-¹. Key parameters, including infection rates, root necrosis indices, plant growth metrics, percentage of fallen trees, and harvest yields, were meticulously monitored and assessed. The results demonstrated that EEOL significantly reduced infection rates (p < 0.05), decreased root necrosis indices (p < 0.05), and promoted increased plant height, pseudostem circumference, and leaf area (p < 0.05) in both models. Furthermore, it lowered the percentage of fallen trees (p < 0.05) and enhanced harvest yields (p < 0.05) in the field model. Notably, observations in the field model revealed that EEOL, particularly at a dosage of 96.54 l · ha-1, exhibited effectiveness equivalent to the conventional chemical nematode control method, fenamiphos (p > 0.05). The study’s findings underscore the promising potential of EEOL in effectively managing R. similis infestations and improving the yield and quality of Cavendish banana plants. The aqueous extract of the stem and leaves of E. odoratum emerged as an effective nematode management solution for banana cultivation, in both the micro plot experiment and field conditions.
Anna Filipiak
The authors have declared that no conflict of interests exist.
Bartholomew E.S., Brathwaite R.A.I., Isaac W.A.P. 2014. Control of root-burrowing nematode (Radopholus similis) in banana using extracts of Azadirachta indica and Allium sativum. Journal of Organic Systems 9 (2): 49–55.
Brooks F.E. 2004. Plant-parasitic nematodes of banana in American Samoa. Nematropica 34 (1): 65–72.
Brooks F.E. 2008. Burrowing Nematode. The Plant Health Instructor. [Available on: https://www.apsnet.org/edcente...]. DOI: https://doi.org/10.1094/PHI-I-....
Cerda H., Carpio C., Ledezma-Carrizalez A.C., Sanchez J., Ramos L., Muñoz-Shugulí C., Andino M., Chiurato M. 2019. Effects of aqueous extracts from amazon plants on Plutella xylostella (Lepidoptera: Plutellidae) and Brevicoryne brassicae (Homoptera: Aphididae) in laboratory, semi-field, and field trials. Journal of Insect Science 19 (5): 8–17. DOI: https://doi.org/10.1093/jisesa....
Chabrier C., Queneherve P. 2003. Control of the burrowing nematode (Radopholus similis Cobb) on banana: impact of the banana field destruction method on the efficiency of the following fallow. Crop Protection 22 (1): 121–127. DOI: https://doi.org/10.1016/S0261-....
Collingborn F.M., Gowen S.R., Mueller-Harvey I. 2000. Investigations into the biochemical basis for nematode resistance in roots of three Musa cultivars in response to Radopholus similis infection. Journal of Agricultural and Food Chemistry 48 (11): 5297–5301. DOI: https://doi.org/10.1021/jf0004....
Desmedt W., Mangelinckx S., Kyndt T., Vanholme B. 2020. A phytochemical perspective on plant defense against nematodes. Frontiers in Plant Sciences 11: 602079. DOI: https://doi.org/10.3389/fpls.2....
Engwali F.D., Nfor T.D., Alain K. 2013. On-farm evaluation of deleafing frequency on the severity of black sigatoka disease (Mycosphaerella fijiensis Morelet) and yield of banana (Musa spp). American Journal of Plant Sciences 3 (3): 595–605. DOI: https://doi.org/10.9734/AJEA/2....
Hölscher D., Dhakshinamoorthy S., Alexandrov T., Becker M., Bretschneider T., Buerkert A., Crecelius A.C., Waele D.D., Elsen A., Heckel D.G., Heklau H., Hertweck C., Kai M., Knop K., Krafft C., Maddula R.K., Matthäus C., Popp J., Schneider B., Schubert U.S., Sikora R.A., Svatoš A., Swennen R.L. 2014. Phenalenone-type phytoalexins mediate resistance of banana plants (Musa spp.) to the burrowing nematode Radopholus similis. Proceedings of the National Academy of Sciences of the United States of America 111 (1): 105–110. DOI: https://doi.org/10.1073/pnas.1....
Jadhav A.B., Kadalag A.D., Amrutsagar V.M. 2019. Effect of integrated nitrogen management on growth and yield of banana on inceptisol. Agricultural Research and Technology 20 (4): 556133. DOI: https:// doi.org/10.19080/ARTOAJ.2019.20.556133.
Jesus F.N., Damasceno J.C.A, Barbosa D.H.S.G, Malheiro R., Pereira J.A., Soares A.C.F. 2015. Control of the banana burrowing nematode using sisal extract. Agronomy for Sustainable Development 35: 783–791. DOI: https://doi.org/10.1007/s13593....
Jitendra P., Shamim Q.M., Kumar G.S., Prasad P.U. 2011. Phytochemical and pharmacological activities of Eupatorium odoratum L. Research Journal of Pharmacy and Technology 4 (2): 184–188.
Kirwa H.K., Murungi L.K., Beck J.J., Torto B. 2018. Elicitation of differential responses in the root-knot nematode Meloidogyne incognita to tomato root exudate cytokinin, flavonoids, and alkaloids. Journal of Agricultural and Food Chemistry 66 (43): 11291–11300. DOI: https://doi.org/10.1021/acs.ja....
Kosma P., Ambang Z., Begoude B.A.D, Ten Hoopen G.M., Kuate J., Akoa A. 2011. Assessment of nematicidal properties and phytochemical screening of neem seed formulations using Radopholus similis, parasitic nematode of plantain in Cameroon. Crop Protection 30 (6): 733–738. DOI: https://doi.org/10.1016/j.crop....
Lamessa K. 2021. Performance evaluation of banana varieties, through farmer’s participatory selection. International Journal of Fruit Science 21 (1): 768–778. DOI: https://doi.org/10.1080/155383....
Marin D.H., Barker K.R., Sutton T.B. 2000. Efficacy of “ABG-9008” against burrowing nematode (Radopholus similis) on bananas. Nematropica 30(1): 1–8.
Motha K.F., Abeysekara R., Kottearachchi N.S. 2010. Effect of biological agents and botanicals in controlling root-knot nematodes, Meloidogyne Spp., in Nicotiana tabacum. Tropical Agricultural Research and Extension 13 (1): 1–6. DOI: https://doi.org/10.4038/tare.v....
Mwaka H.S., Bauters L., Namaganda J., Marcou S., Bwesigye P.N., Kubiriba J., Smagghe G., Tushemereirwe W.K., Gheysen G. 2023. Transgenic East African highland banana plants are protected against Radopholus similis through host-delivered RNAi. International Journal of Molecular Sciences 24 (15): 12126. DOI: https://doi.org/10.3390/ijms24....
Rahman M., Begum R., Surag A.T., Tusher M.S.H., Huda M.K. 2023. Uncovering the phytochemical profile, antioxidant potency, anti-inflammatory effects, and thrombolytic activity in Dendrobium lindleyi Steud. Scientifica 2023: 9999640. DOI: https:// doi.org/10.1155/2023/9999640.
Ramya R., Dhamotharan R. 2015. Qualitative and quantitative analysis of phytochemicals of Costus speciosus. International Journal of Health Sciences and Research 5 (12): 170–176. DOI: https://doi.org/10.13140/RG.2.....
Sankar C., Soorianathasundaram K., Kumar N., Karunakaran G., Sivakumar M. 2017. Induction of resistant to radopholus similis and defence related mechanism in susceptible and resistance banana hybrids infected with Radopholus similis. International Journal of Current Microbiology and Applied Sciences 6 (4): 1668–1684. DOI: https://doi.org/10.20546/ijcma....
Sarah J., Pinochet J., Stanton J. 1996. The Burrowing Nematode of Bananas, Radopholus Similis Cobb, 1913. Musa Pest Fact Sheet (INIBAP), Montpellier, France, 129 pp.
Sora S.A., Guji M.J. 2023. Evaluation of banana (Musa Spps.) for growth, yield, and disease reaction at Teppi, Southwestern Ethiopia. International Journal of Fruit Science 23 (1): 62–69. DOI: https://doi.org/10.1080/155383....
Thoden T.C., Boppré M. 2010. Plants producing pyrrolizidine alkaloids: sustainable tools for nematode management? Nematology 12 (1): 1–24. DOI: https://doi.org/10.1163/138855....
Udebuani A.C, Abara P.C., Obasi K.O., Okuh S.U. 2015. Studies on the insecticidal properties of Chromolaena odorata (Asteraceae) against adult stage of Periplaneta americana. Journal of Entomology and Zoology Studies 3 (1): 318–321.
Veech J.A. 1979. Histochemical localization and nematoxicity of terpenoid aldehydes in cotton. Journal of Nematology 11 (3): 240–246.
Wang K.H., Uyeda J., Sugano J. 2001. Banana Pest and Disease Management in the Tropical Pacific: A guidebook for banana growers. College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, Hawaii, USA, 72 pp.
Wezel A., Casagrande M., Celette F., Vian J.F., Ferrer A., Peigné J. 2014. Agroecological practices for sustainable agriculture. A review. Agronomy for Sustainable Development 34: 1–20. DOI: https:// doi.org/10.1007/s13593-013.0180-7.
Zachariades C., Day M., Muniappan R., Reddy G.V.P. 2009. Chromolaena odorata (L.) King and Robinson (Asteraceae). p. 130–162. In: “Biological Control of Tropical Weeds Using Arthropods” (R. Muniappan, G.V.P. Reddy, A. Raman, eds.). Cambridge University Press, UK.
Journals System - logo
Scroll to top