ORIGINAL ARTICLE
Incidence and genetic diversity of raspberry bushy dwarf virus and raspberry leaf blotch virus isolates in red raspberry in Ukraine
1
Department of Virology, Sanitation and Propagation of Fruit and Berry Crops, Institute of Horticulture, NAAS of Ukraine, Kyiv, Ukraine
2
Gene Pool, Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech Republic
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2024-10-01
Acceptance date: 2025-02-18
Online publication date: 2026-09-07
Corresponding author
Iryna Riaba
Department of Virology, Sanitation and Propagation of Fruit and Berry Crops, Institute of Horticulture, NAAS of Ukraine, Kyiv, Ukraine
Department of Virology, Sanitation and Propagation of Fruit and Berry Crops, Institute of Horticulture, NAAS of Ukraine, Kyiv, Ukraine
HIGHLIGHTS
- The study analysed RBDV and RLBV isolates in Ukraine using molecular-genetic methods
- Surveys in nine regions revealed a 33.3% infection rate in raspberry plants
- Fragments of four RBDV and two RLBV isolates were sequenced and deposited in GenBank
- Phylogenetic analysis showed a high genetic diversity of isolates from Ukraine
KEYWORDS
phylogenetic analysisRubus idaeus Lraspberry bushy dwarf virus (RBDV)raspberry leaf blotch virus (RLBV)RT-PCR
TOPICS
ABSTRACT
The objective of this study was to assess the prevalence and genetic diversity of raspberry
bushy dwarf virus (RBDV) and raspberry leaf blotch virus (RLBV) in Ukrainian raspberry
plantations. A survey of viral symptoms, including yellow leaf blotch, leaf distortion, mottling,
and crumbly fruits, was conducted across several regions. Crumbly fruits were linked
to RBDV infection, while yellow leaf blotch and leaf distortion were associated with RLBV
and the presence of eriophyid mites, which serve as RLBV vectors. To detect the presence
of these viruses, 195 symptomatic and asymptomatic leaf samples were collected from cultivated
raspberry fields and tested using RT-PCR. The overall infection rate was 33.3%, with
RBDV detected in 29.2% of the samples and RLBV in 4%. Comparison of sequences of the
Ukrainian RBDV isolates showed high nucleotide and amino acid identity and grouped
them with isolates from Belarus, Sweden, and Kazakhstan. RLBV isolates from Ukraine
displayed notable genetic variability, clustering with isolates from Finland, Serbia, and Slovakia.
These findings highlight a high level of virus infections in raspberry plantations in
Ukraine. Despite the obligatory certification of planting material, RBDV remains a significant
threat to raspberry farming. Furthermore, the emergence and spread of RLBV in
Ukraine could further worsen the situation.
FUNDING
This study was conducted as part of project
19.01.02.01.F “Development of theoretical basics of
the production systems and certification of fruit, berry,
and ornamental planting material on a virus-free
basis” (0116U000653, 2016-2020) and 14.00.03.03.F
«Development of biotechnological basics of production,
propagation and quality control of fruit
and berry crops planting material” (0121U108148,
2021–2025) of the National Academy of Agrarian
Sciences of Ukraine.
RESPONSIBLE EDITOR
Assunta Bertaccini
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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| ISSN: | 1427-4345 |
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