ORIGINAL ARTICLE
Phenotypic and molecular characterization of the causal agent of chafer beetle mortality in the wheat fields of the Kurdistan province, Iran
| 1 | Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran |
| 2 | Plant Protection Department, Agricultural and Natural Resources Research Center of Kurdistan, P.O. Box 714,
Sanandaj, Iran |
CORRESPONDING AUTHOR
Keivan Karimi
Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
Plant Protection Department, Agricultural Faculty, University of Tabriz, P.O. Box 5166614766, Tabriz, Iran
Submission date: 2014-09-09
Acceptance date: 2015-06-19
Journal of Plant Protection Research 2015;55(3):227–234
KEYWORDS
TOPICS
ABSTRACT
We report the first case of chafer beetle [Anisoplia austriaca
(Herbst 1783)] mortality caused by
Actinomucor elegans
var.
elegans
in wheat fields of the Kurdistan province, Iran. For three years, dead larvae of
Anisoplia austriaca
were collected from wheat fields of
the Kurdistan province. Similar isolates of a fast-growing fungus were recovered from all samples. The fungal isolates were identified
as
A. elegans
var.
elegans
based on morphological and cultural characteristics. The identity of the species was further confirmed using
sequence data of the ITS (Internal Transcribed Spacer) region of ribosomal DNA. Koch’s postulates were fulfilled by the inoculation
of the larvae of
A. austriaca
and
Galleria mellonella
(Linnaeus, 1758) (as the model insect) using the spore suspension of
A. elegans
var.
elegans. The viability of sporangiospores was evaluated using a spore dilution technique on germination medium.
The results on the
pathogenicity (100% mortality in
A. austriaca
larvae) and viability tests (germination: 95.45%) demonstrated that
A. elegans
var.
elegans
can be considered as a potential biocontrol agent against the chafer beetle. Field experiments are still required to evaluate the capacity
of
A. elegans
as a biological control agent.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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