Survival rate, insecticidal and fungistatic activity of antagonistic actinomycete Streptomyces griseoviridis and entomopathogenic fungus Beauveria bassiana in separate and combined introductions to the soil
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Centre for Ecological Research PAS, Dziekanów Leśny, M. Konopnickiej 1, 05-092 Łomianki, Poland
Institute of Microbiology, BNAS, Kuprevich 2, 220141 Minsk, Belarus
Corresponding author
Anna Augustyniuk-Kram
Centre for Ecological Research PAS, Dziekanów Leśny, M. Konopnickiej 1, 05-092 Łomianki, Poland
Journal of Plant Protection Research 2007;47(2):179-186
Highly active antagonistic actinomycete Streptomyces griseoviridis and entomopathogenic fungus Beauveria bassiana were applied to the soil separately and together (in association) in the laboratory experiments. We assessed survival rate, insecticidal and fungistatic activity of these strains. We also tested the influence of synthetic insecticide Regent 25® (fipronil 25g/l) on investigated parameters. Additionally, insecticidal activity of both strains was compared with insecticidal activity of Regent. It was shown that both strains, especially S. griseoviridis, good survived in soil. Population density of S. griseoviridis in the association with B. bassiana increased 2–3 times compared to initial density. Regent considerably reduced population density of S. griseoviridis and B. bassiana. Insecticidal efficiency of S. griseoviridis was comparable with the effect of synthetic incecticide Regent and reached 89.2% and 86.8% respectively. Fungistatic activity towards Fusarium oxysporum showed only S. griseoviridis and it was observed that this activity decreased in time course.
The authors have declared that no conflict of interests exist.
Anderson L.E., Ehrlich J., Sun S.H., Burkholder P.R. 1956. Strains of Streptomyces the sources of azaserine, elaiomycin, griseoviridin and viridiogrisein. Antibiot Chemother 6: 100–115.
Bochow H. 1989. Use of microbial antagonists to control soil-borne pathogens in greenhouse crops. Acta Hortic. 255: 271–280.
Cao L., Qiu Z., You J., Tan H., Zhou S. 2005. Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol. Lett. 274: 147–152.
Crawford D.L., Lynch J.M., Whipps J.M., Ousley M.A. 1993. Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl. Environ. Microbiol. 59 (11): 3899–3905.
Das A.C., Mukherjee D. 2000. Soil application of insecticides influences microorganisms and plant nutrients. Appl. Soil Ecol. 14: 55–62.
Egorov H.C. 1986. Osnovy Uczenija ob Antibiotikach. Wysšaja škola, Moskwa: 117–154 (in Russian).
Jung K. 2005. Biological control of Thrips tabaci in the field – possibilities and practical limits. p. 81. In:Proceedings of 10th European Meeting “Invertebrate pathogens in biological control: present and future“. Locorotondo, 10–15 June, 2005.
Kandybin N.V., Smirnov O.V. 1996. Novel ecologically safe biopesticides against insects and mites. IOBC/WPRS Bull. 19 (9): 15–17.
Kolomiets E.I., Zdor N.A., Romanovskaya T.V., Lobanok A.G., Adamovich O. 1999. Streptomyces griseoviridis as the antagonist of plant pathogens. Bull. Pol. Acad. Sci. 47 (2–4): 89–97.
Lahdenpera M.L., Simon E., Uoti J. 1991. Mycostop – a novel biofungicide based on Streptomyces bacteria. p. 258–263. In: ”Biotic Interactions and Soil-Borne Disease” (A.B.R. Beemster, G.J. Bollen, M. Gerlagh, M.A. Ruissen, B. Schippers, A. Tempel, eds.) Elsevier, Amsterdam, The Netherlands Lobanok A.G., Kolomiets E.I. 2000. Microbial pesticides: from laboratory studies to practice. p. 181–182. In: “Microbiology and Biotechnology on the Eve of XXI Century” (A.G. Lobanok, L.I. Stepanovich, eds.). Propilei, Minsk.
Lockwood J.L. 1959. Streptomyces spp. as a cause of natural fungitoxicity in soils. Phytopathology 49: 327–331.
Martinez-Toledo M.V., Salmeron V., Gonzalez-Lopez J. 1992. Effect of an organophosphorus insecticide, phenophos on agricultural soil microflora. Chemosphere 24: 71–80.
Minuto A., Spadaro D., Garibaldi A., Gullino M.L. 2006. Control of soilborne pathogens of tomato using a commercial formulation of Streptomyces griseoviridis and solarization. Crop Prot. 25: 468–475.
Pędziwilk Z. 1995. The numbers and fungistatic activity of actinomycetes in different soils supplemented with pesticides and organic substances. Pol. J. Soil Sci. 28: 45–52.
Pepper I.L., Gerba C.P., Brendecke J.W. 1995. Environmental Microbiology. A Laboratory Manual. Academic Press, San Diego, 175 pp.
Popowska-Nowak E., Bajan C., Augustyniuk-Kram A., Kolomiets E.I, Chikileva A., Lobanok A.G 2003. Interactions between soil microorganisms: bacteria actinomycetes and entomopathogenic fungi of the genera Beauveria and Paecilomyces. Pol. J. Ecol. 51 (1): 85–90.
Samac D.A., Willert A.M., McBride M.J., Kinkel L.L. 2003. Effects of antibiotic producing Streptomyces on nodulation and leaf spot of alfalfa. Appl. Soil Ecol. 22: 55–66.
Shoda M. 2000. Bacterial control of plant diseases. J. Biosci. Bioeng. 89 (6): 515–521.
Szegi J. 1983. Opriedjelenje stimuljatorow i ingibitorow w kulturach mikoorganizmow. p. 167–168. In: “Metody Poczwiennoj Mikrobiołogii” (J. Szegi, ed.). Kolos, Moskwa.
Williamson S.T., Goodfellow M., Alderson G. 1989. Genus Streptomyces Waksman and Henrici 1943.339 AL. p. 2452–2492. In: ”Bergey’s Manual of Systematic Bacteriology” (S.T. Williams, M.E. Share, J.G. Holt, eds.). Vol. 4. Williams and Wilkins,Baltimore.
Wraight S.P., Ramos M.E. 2005. Synergistic interaction between Beauveria bassiana and Bacillus thuringiensis tenebrionis – based biopesticides applied against field populations of Colorado potato beetle larvae. J. Invertebr. Pathol. 90: 139–150.
Yi X., Ehlers R.U. 2005. Combining Steinernema carpocapsae and Bacillus thuringiensis strains for control of Diamondback Moth (Plutella xylostella). p.163. In: Proceedings of 10th European Meeting “Invertebrate pathogens in biological control: present and future“. Locorotondo, 10–15 June, 2005.
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