ORIGINAL ARTICLE
Antifungal activity of 2-hydroxy-4-methoxybenzaldehyde isolated from Decalepis hamiltonii (Wight & Arn.) on seed-borne fungi causing biodeterioration of paddy
 
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1
Agricultural Microbiology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore, India
2
Agricultural Microbiology Laboratory, Department of Microbiology, Bangalore University, Jnana Bharathi Campus, Bangalore, India
CORRESPONDING AUTHOR
Devihalli Chikkaiah Mohana
Agricultural Microbiology Laboratory, Department of Microbiology, Bangalore University, Jnana Bharathi Campus, Bangalore, India
 
Journal of Plant Protection Research 2009;49(3):250–256
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ABSTRACT
In vitro antifungal activity assay of different concentrations of 2-hydroxy 4-methoxybenzaldehyde isolated from Decalepis hamiltonii against six important seed-borne fungal pathogens viz., Alternaria alternata, Drechslera tetramera, Fusarium oxysporum, F. proliferatum, Pyricularia oryzae and Trichoconis padwickii isolated from paddy seeds revealed that, the compound 2-hydroxy-4-methoxybenzaldehyde showed significant antifungal activity. Among the fungi tested, F. proliferatum showed highest inhibitory activity, whereas P. oryzae showed least inhibitory activity. The minimal inhibitory concentration (MIC) varied between 350 μg/ml and 650 μg/ml depending on the fungal species. Comparative evaluation of the active compound with the synthetic fungicide thiram at recommended dosage revealed that, the antifungal activity of the active compound obtained from the plant was almost equivalent. Evaluation for nutritional parameters and dry matter losses (DML) revealed that, total carbohydrates, water soluble proteins, lipids and dry matter losses were significantly confined in 2-hydroxy-4-methoxybenzaldehyde treated paddy seeds compared with control seeds. This plant being an edible one can be exploited in the management of seed-borne pathogenic fungi and in the prevention of biodeterioration of grains and mycotoxin production during storage in an eco-friendly way.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (30)
1.
Agrawal R.L. 1999. Seed Technology. 2nd ed. New Delhi: Oxford and IBH Publishing Co.: 87–97.
 
2.
Agrios G.N. 1997. Plant Pathology. 4th ed. California: Academic Press. 245–269.
 
3.
Aliero A.A., Afolayan A.J. 2006. Antimicrobial activity of Solanum tomentosum. Afri. J. Biotechnol. 5: 369–372.
 
4.
Anonim. 1952. The wealth of India. First supplemented series (raw material). Vol. 1. NISC and CSIR , New Delhi, India.
 
5.
Anonim. 2005. Pest control background. Int. J. Pest Control 45: 232–233.
 
6.
Bagga P.S., Sharma V.K. 2006. Evaluation of fungicides as seedling treatment for controlling bakanae/foot-rot (Fusarium moniliforme) disease in basmati rice. J. Mycol. Plant Pathol. 59: 305–308.
 
7.
Buwa L.V., Staden J.V. 2006. Antibacterial and antifungal activity of traditional medicinal plants used against venereal diseases in South Africa. J. Ethnopharmacol. 103: 139–142.
 
8.
Chandler J. 2005. Cost reduction in SIT programmes using exosect auto-dissemination as part of area wide integrated pest management. Int. J. Pest Control 47: 257–260.
 
9.
Domijan A., Feraica M., Jurjevic Z., Ivil D., Cvjetkovic B. 2005. Fumonisin B1, fumonisin B2, zearalenone and ochratoxin A contamination of maize in Croatia. Food Additives and Contaminants 22: 677–680.
 
10.
Dubois M., Gilles K.A., Hamilton J.K., Robers P.A., Smith F. 1956. Colorimetric method for determination of sugar and related substances. Anal. Chem. 28: 350–353.
 
11.
Elizabeth K.M., Vimala Y., Devarapalli H.C.P. 2005. Antimicrobial activity of Decalepis hamiltonii. Asian J. Microbiol. Biotechnol. Environ. Sci. 7: 151–53.
 
12.
Ergene A., Guler P., Tan S., Mirici S., Hamzaoglu E., Duran A. 2006. Antibacterial and antifungal activity of Heracleum sphondylium subsp. A rtvinense. Afr. J. Biotechnol. 5: 1087–1089.
 
13.
Fabbri A.A., Fanelli C., Serafini M. 1980. Aflatoxin production in cereals oil seeds and some organic fractions extracted from sunflower. Acad. Neuzeeland Sci. 98: 219–228.
 
14.
George J., Bais H.P., Ravishankar G.A. 2000. Biotechnological Production of Plant-Based Insecticides. Crit. Revi. Biotechnol. 49: 49–77.
 
15.
George J., Ravishankar G.A., Keshava N., Udayasankar K. 1999a. Antibacterial activity of supercritical extract from Decalepis hamiltonii root. Fitoterapia 70: 172–174.
 
16.
George J., Ravishankar G.A., Pereira J., Divakar S. 1999b. Bioinsecticide from swallowroot (Decalepis hamiltonii) Wight & Arn protects food grains against insect infestation. Curr. Sci. 77: 501–502.
 
17.
Ghasolia R., Jain C. 2004. Evaluation of fungicides, bio-agents, phyto-extracts and physical seed treatment against Fusarium oxysporum f.sp. Cumini wilt in Cumin. J. Mycol. Plant Pathol. 34: 334–336.
 
18.
ISTA. 1996. International Rules for Seed testing. Seed Sci. Technol. 21: 25–30.
 
19.
Lowry O.H., Rosebrough N. J., Farr A. L., Rauoll R. J. 1951. Protein measurement with the folin-phenol reagent. J. Biol. Chem. 193: 256–277.
 
20.
Mohana D.C., Raveesha K.A., Lokanath Rai. 2008. Herbal remedies for the management of seed-borne fungal pathogens by an edible plant Decalepis hamiltonii(Wight & Arn). Archi. Phytopathol. Plant Prot. 41(1): 38–49.
 
21.
Nagarajan S., Jagan Mohan Rao L., Gurudatt K.N. 2001. Chemical composition of the volatiles of Decalepis hamiltonii(Wight & Arn). Flavour Fragrance J. 16: 27–29.
 
22.
Parekh J., Karathia N., Chanda S. 2006. Evaluation of antibacterial activity and phytochemical analysis of Bauhinia variegata L. bark. Afr. J. Biomed. Res. 9: 53–56.
 
23.
Phadke N.Y., Gholap A. S., Ramakrishnan K., Subbulakshmi G. 1994. Essential oil of Decalepis hamiltonii as an antimicrobial agent. J. Food Sci. Technol. 31: 472–475.
 
24.
Reed C. 1987. The precision and accuracy of the standard volume weight method of estimation of dry weight losses in wheat grain, sorghum and maize and a comparison with the thousand grain mass method in wheat containing fine material. J. Stored Products Res. 23: 223–231.
 
25.
Rocha O., Ansari K., Doohan F.M. 2005. Effect of trichothecene mycotoxins on eukaryotic cells : A review. Food Additives and Contaminants22:369–378.
 
26.
Sagar V., Sugha S.K. 2004. Effect of seed dressing fungicides and soil compaction on root rot diseases complex and yield in pea. J. Mycol. Plant Pathol. 34: 892–895.
 
27.
Satish S., Raveesha K.A., Janardhana G.R. 1999. Antibacterial activity of plant extracts on phytopathogenic Xanthomonas campestris pathovars. Letters Appli. Microbiol. 28: 145–147.
 
28.
Singh J., Tripathi N.N. 1999. Inhibition of storage fungi of blackgram (Vigna mungo L.) by some essential oils. Flavour Fragrance J.14: 1–4.
 
29.
Thangadurai D., Anita S., Pullaiah T., Reddy P.N., Ramachandraiah O.S. 2002. Essential oil constituents and in vitro Antibacterial Activity of Decalepis hamiltonii roots against Foodborne Pathogens. J. Agricul. Food Chem. 50: 3147–3149.
 
30.
Varma J., Dubey N.K. 1999. Prospectives of botanical and microbial products as pesticides of tomorrow. Curr. Sci. 76: 172–179.
 
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