ORIGINAL ARTICLE
Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
| 1 | Centro de Investigaciones de Fitopatología (CIDEFI-CICBA)., Facultad de Ciencias Agrarias y Forestales. Universidad Nacional de La Plata. Calle 60 y 119 s/n, 1900, La Plata, Buenos Aires, Argentina |
| 2 | Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP-CONICET)., Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 1900, La Plata, Buenos Aires, Argentina |
| 3 | Centro de Investigaciones de Fitopatología (CIDEFI-CICBA),, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata. 1900, La Plata, Buenos Aires, Argentina. |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Andrea Vanesa Toledo
Centro de Investigaciones de Fitopatología (CIDEFI-CICBA),, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata. 1900, La Plata, Buenos Aires, Argentina., Avda. 60 y 119 s/n, 1900 La Plata, Argentina
Centro de Investigaciones de Fitopatología (CIDEFI-CICBA),, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata. 1900, La Plata, Buenos Aires, Argentina., Avda. 60 y 119 s/n, 1900 La Plata, Argentina
Submission date: 2017-10-02
Acceptance date: 2017-12-01
Journal of Plant Protection Research 2018;58(1):45–53
KEYWORDS
TOPICS
ABSTRACT
Many species of Trichoderma produce secondary metabolites such as volatile organic compounds (VOCs) that reduce plant diseases and promote their growth. In this work we evaluated the antagonistic effect of VOCs released by eight strains of two Trichoderma species against Pyrenophora teres Drechsler, the causal agent of barley net blotch. Antagonism was estimated based on the percentage of mycelial growth inhibition according to the confronted cultures method. VOCs extraction and identification were performed b y gas chromatography and mass spectrometry, through different methodologies for VOCs emitted by antagonists and pathogens alone or when confronted. VOCs produced by all Trichoderma strains inhibited mycelial growth of the pathogen in a range of 3% to 32%, showing weak and unpigmented mycelia with vacuolization. In addition, P. teres stimulated the release of VOCs by both Trichoderma species. The major groups of VOCs detected were sesquiterpenes, followed by diterpenes, terpenoids and eight-carbon compounds. This is the first report about characterization of volatiles emitted by Trichoderma in presence of P. teres.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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