ORIGINAL ARTICLE
Antifungal activity of bioactive compounds produced by the endophyte Bacillus velezensis NC318 against the soil borne pathogen Sclerotium rolfsii Sacc
1
Plant Protection Unit, Hassan II Agronomic and Veterinary Institute, Rabat, Morocco
2
Plant Protection Unit, National Institute of Agronomic Research, Rabat, Morocco
3
Anti-Doping Control Laboratory, Hassan II Agronomic and Veterinary Institute, Kenitra, Morocco
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2022-06-09
Acceptance date: 2022-09-02
Online publication date: 2022-10-21
Corresponding author
Michelle Gaëlle Siméone Bidima
Plant Protection Unit, Hassan II Agronomic and Veterinary Institute, Rabat, Morocco
Plant Protection Unit, Hassan II Agronomic and Veterinary Institute, Rabat, Morocco
Journal of Plant Protection Research 2022;62(4):326-333
HIGHLIGHTS
- Bacillus velezensis NC318 produced potent antifungal compounds against the plant pathogenic fungus Sclerotium rolfsii Sacc.
- The secreted antifungal compounds could inhibit mycelial growth and sclerotia germination of the pathogen.
- Lipopeptides, polyketides, and siderophores are involved in the control of the pathogen.
- B. velezensis NC318 could be a promising biological control agent.
KEYWORDS
TOPICS
ABSTRACT
In a previous study, the endophytic Bacillus velezensis NC318 was isolated from the rhizo-
sphere of date palm and showed strong antifungal activity against the soil-borne plant
pathogenic fungus, Sclerotium rolfsii Sacc, the causal agent of Southern blight. The poten-
tial of the Bacillus genus in the inhibition of plant pathogens is mainly due to the produc-
tion of certain bioactive compounds. In the present study, secondary metabolites extracted
from the cell-free supernatant of strain NC318 showed strong antifungal activity on the
mycelial growth and germination of S. rolfsii sclerotia in vitro. With 50 μl of bioactive com-
pounds crude extracts, the mycelial growth inhibition rate was 97% and any germination
of sclerotia was reported. Chemical analysis of the secondary metabolite crude extracts
performed by high performance liquid chromatography coupled with mass spectrometry
(HPLC/MS), revealed that the secreted bioactive compounds belonged to the family of li-
popeptides (iturin, fengycin, surfactin), polyketides (bacillaene, macrolactin, difficidin and
bacilysin) and siderophores (bacillibactin). These results provide a better understanding of
the biocontrol mechanism of the bacteria strain B. velezensis NC318 against the soil fungal
pathogens, especially S. rolfsii root rot.
RESPONSIBLE EDITOR
Joanna Puławska
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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