ORIGINAL ARTICLE
Cotton leaf blight disease caused by Alternaria alternata in Sudan
1
Plant Pathology Department, Integrated Pest Management Center, Agricultural Research Corporation, Wad Medani, Sudan
2
Biotechnology and Biosafety Research Center, Agricultural Research Corporation, Khartoum, Sudan
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
Submission date: 2018-12-02
Acceptance date: 2019-08-26
Online publication date: 2019-10-11
Corresponding author
Nafisa Elmahi Ahmed
Plant Pathology Department, Integrated Pest Management Center, Agricultural Research Corporation, Wad Medani, Sudan
Plant Pathology Department, Integrated Pest Management Center, Agricultural Research Corporation, Wad Medani, Sudan
Journal of Plant Protection Research 2019;59(3):412-417
KEYWORDS
Alternaria alternataconidiaGossypium sppinternal transcribed spacer (ITS)morphological variabilitypathogenicity
TOPICS
ABSTRACT
Genetically modified Bt cotton (Gossypium hirsutum) leaves with typical symptoms of Alternaria
early blight disease resembling that of tomato and potato were observed in the
main cotton growing schemes in Sudan. Symptoms on leaves appeared as either brown
2leaf spot with gray centers or leaf blight with concentric rings. Pathogenicity tests using
isolates with both symptoms showed that the isolated fungi were highly pathogenic
to both G. hirsutum and G. barbadense cotton varieties. Alternaria alternata isolated from
infected tomato and potato leaves with early blight symptoms was included for comparison.
Microscopic examination showed that the mean length of conidia from cotton, tomato
and potato isolates ranged from 26.25 to 45.45 μm, while the width ranged from 9.56 to
13.64 μm. The mean number of transverse septa among all isolates was 3.4 to 5.7 and the
peak length ranged from 3.75 to 7.8 μm. Based on morphological characteristics the two
isolates from cotton were identified as A. alternata. Genomic DNA was extracted directly
from fungal cultures grown on potato dextrose agar (PDA) plates using a Zymo Research
Quick DNA kit. A species-specific primer using the internal transcribed spacer ribosomal
DNA (ITS rDNA) PCR scoring indicated the presence of A. alternata using primer pair
ITS4/ITS5. Amplifications of the internal transcribed spacer region of 600 bp revealed
100% identity of the isolated fungus from cotton with A. alternata from tomato and potato.
These data oblige us to reconsider the presence of A. alternata in the four main cotton
growing schemes in Sudan while these symptoms have always been described for tomato and potato early blight disease.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
REFERENCES (20)
1.
Ahmed N.E., Gamiel S., Kurmut A.M., Adam S.M., Mohamed S.Y. 2006. Evaluation of Ridomil Gold MZ (Metalaxyl + Mancozeb) to control early blight caused by Alternaria alternata in tomato. p. 57–61. In: Proceedings of the National Pests and Diseases Committee, Meeting No 75, Wad Medani, Sudan.
2.
Bashan Y., Levanony H. Or R. 1991. Association between Alternaria macrospora and Alternaria alternata, causal agents of cotton leaf blight. Canadian Journal of Botany 69 (12): 2603–2607. DOI: https: //doi.org/10.1139/b91-324.
3.
Chelkowski J., Visconti A. 1992. Alternaria Biology, Plant Diseases and Metabolites. Topics in Secondary Metabolism Volume 3, 573 pp.
5.
Ephrath J.E., Shtienberg D., Drieshpoun J., Dinoor A., Marani A. 1989. Alternaria alternata in cotton (Gossypium hirsutum) cv. Acala: Effects on gas exchange, and yield components. European Journal of Plant Pathology 95 (3): 157–166. DOI: https: //doi: 10.1007/BF01999971.
6.
Guo L.D., Xu L., Zheng W.H., Hyde K.D. 2004. Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatellites (RAMS). Fungal Diversity 16: 53–65.
7.
Hubballi M., Nakkeeran S., Raguchander T., Rajendran L., Renukadevi P. Samiyappan R. 2010. First report of leaf blight of onion caused by Alternaria alternata (Fr.) Keissler. Journal of General Plant Pathology 76 (4): 284–286. DOI: https: //doi: 10.1007/s10327-010-0240-7.
8.
Kaur M., Aggarwal N.K. 2015. First report of Alternaria spp. causing leaf blight disease on parthenium weed in India. New Disease Reports 31: 25. DOI: https: //doi: 10.5197/J.2044-0588.
9.
Kemmitt G. 2002. Early blight of potato and tomato. The Plant Health Instructor. DOI: https: //doi: 10.1094/PHI-I-2002-0809-01.
10.
Khalid D.A., Ahmed N.E. Mohamed Y.S. 2004. Studies on Early Blight Disease on Tomato Caused by Alternaria alternata. The Second National Pest Management Conference in the Sudan. Abstract book, 90 pp.
11.
Laidou I.A., Koulakiotu E.K., Thanassoulopoulos C.C. 2000. First report of stem canker Caused by Alternaria alternata on cotton. Plant Disease 84 (1): 103. DOI: https: //doi:10.1094/PDIS.2000.84.1.103A.
12.
Liu W., Zhang W., Zhang N., Zhai W. 2018. Study of cotton leaf senescence induced by Alternaria alternata infection. p. 161–171. In: Plant Senescence (Guo Y., ed.). Methods in Molecular Biology. Human press, New York, 367 pp. DOI: https: //doi: 10.1007/978-1-4939-7672-0-15.
13.
Maas S., Allen S., Weir D. 2012. Cotton Symptoms Guide. The Guide to Symptoms of Diseases and Disorders in Australian cotton. 1st Edition. A Production of the Australian Cotton Industry Development and Delivery Team, 81 pp.
14.
Maiti C.K., Sen S., Acharya R., Acharya K. 2007. First report of Alternaria alternata causing leaf spot on Stevia rebaudiana. Plant Pathology 56 (4): 723. DOI: https: //doi.org/10.1111/j.1365-3059.2007.01578.x.
15.
Olanya O.M., Honeycutt C.W., Larkin R.P., Griffin T.S., He Z., Halloran J.M. 2009. The effect of cropping systems and irrigation management on development of potato early blight. Journal of General Plant Pathology 75 (4): 267–275. DOI: 10.1007/s10327-009-0175-z.
16.
Pryor B.M., Michailides T.J. 2002. Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 92 (4): 406–416. DOI: https://doi.org /10.1094/ Phyto.2002.92.4.406.
17.
Sharma M., Ghosh R., Pande S. 2013. Occurrence of Alternaria alternata causing Alternaria blight in pigeonpea in India. Advances in Bioscience and Biotechnology 4 (6): 702–705. DOI: https: // doi: 10.4236/abb.2013.46092.
18.
van der Waals J.E., Korsten L., Aveling T.A.S. 2001. A review of early blight of potatoes. African Plant Protection70: 91–102. DOI: https://doi:10.12691/wjar-5-3-....
19.
Venkatesh I. 2014. Studies on Alternaria Leaf Spot of Cotton. Acharya N.G. Ranga Agricultural University, Guntur, India. M Sc Thesis in Plant Pathology, 106 pp.
20.
Zhao J., Li S., Jiang T., Liu Z., Zhang W., Jian G., Qi F. 2012. Chilling stress, the key redisposing factor for causing Alternaria alternata infection and leading to cotton (Gossypium hirsutum L.) leaf senescence. PLoS ONE 7 (4): e3616. DOI: 10.1371/journal.pone.0036126.Epub 2012.
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| eISSN: | 1899-007X |
| ISSN: | 1427-4345 |
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