ORIGINAL ARTICLE
Morphological and molecular identification of cyst nematode species (Heterodera spp.) in Algerian cereal fields
| 1 | Department of Agricutural and Forestry Zoology, National High School of Agronomy (ENSA), Frères Ouadek, Hacen Badi, El-Harrach, Algeria |
| 2 | National Institute of Agricultural Research (INRA-Rabat), Biotechnology Research Unit, Avenue Mohamed Belarbi Alaoui, Rabat, Morocco |
| 3 | Faculty of Agriculture and Natural Science, Department of Plant Protection, Bolu Abant Izzet Baysal University, Golkoy, Bolu, Turkey |
| 4 | Faculty of Sciences, Department of Agronomic Sciences, University of Khemis Miliana, Theniet El Had Road, Ain Defla, Algeria |
| 5 | Plant Protection Department, International Maize and Wheat Improvement Center (CIMMYT), Emek, Ankara, Turkey |
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Fouad Mokrini
National Institute of Agricultural Research (INRA-Rabat), Biotechnology Research Unit, Avenue Mohamed Belarbi Alaoui, Rabat, Morocco
National Institute of Agricultural Research (INRA-Rabat), Biotechnology Research Unit, Avenue Mohamed Belarbi Alaoui, Rabat, Morocco
Submission date: 2019-03-15
Acceptance date: 2019-05-07
Online publication date: 2019-10-11
Journal of Plant Protection Research 2019;59(3):400–411
KEYWORDS
TOPICS
ABSTRACT
Cereal cyst nematodes (Heterodera spp.) are distributed globally and cause severe production
losses of small grain cereals. To investigate the occurrence of cereal cyst nematodes
in wheat-growing areas of Algeria, a survey was conducted and 27 cereal cyst nematode
populations were collected. The populations were initially identified based on their morphological
and morphometric characters, followed by molecular methods using speciesspecific
primers, complemented by ITS-rDNA sequences. The morphological and morphometric
features of second-stage juveniles (J2s) and cysts supported the presence of three
Heterodera species: H. avenae, H. filipjevi and H. hordecalis. All morphological values of
these distinct populations were very similar to those previously described for these species.
Using species-specific primers for H. avenae and H. filipjevi, the specific bands of 109 bp and
646 bp confirmed the morphological identification of both species, respectively. In addition,
the internal transcribed spacer (ITS) regions were sequenced to study the diversity of
the 27 populations. These sequences were compared with those of Heterodera species available
in the GenBank database (www.ncbi.nlm.nih.gov) and re-confirmed the identity of
the species. Nineteen sequences of ITS-rDNA were similar (99–100%) to the sequences of
H. avenae published in the GenBank, six sequences were similar (99–100%) to H. hordecalis,
and two were similar (98–99%) to H. filipjevi. The results of this study are of great value
to breeding programs and extension services, where they will contribute to the design of
control measures to keep damaging nematodes in check.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
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