RAPID COMMUNICATION
Fusarium graminearum sensu stricto associated with head blight on rye (Secale cereale L.) in Argentina
Mauro Martinez 1, B-D,F
,
 
Maria Dinolfo 1, B-D,F
,
 
,
 
Sebastian Stenglein 1, A-C,E-F
 
 
 
More details
Hide details
1
Functional Biology and Biotechnology Laboratory, Faculty of Agronomy, National University of the Centre of the Buenos Aires Province, Argentina
 
 
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
 
 
Submission date: 2018-09-26
 
 
Acceptance date: 2019-05-09
 
 
Online publication date: 2019-06-04
 
 
Corresponding author
Sebastian Stenglein   

Functional Biology and Biotechnology Laboratory, Faculty of Agronomy, National University of the Centre of the Buenos Aires Province, Argentina
 
 
Journal of Plant Protection Research 2019;59(2):287-292
 
KEYWORDS
TOPICS
ABSTRACT
Fusarium head blight (FHB) is one of the most important diseases that occurs in cereal regions worldwide and causes serious economic damage. This disease can be caused by several Fusarium species with Fusarium graminearum sensu stricto being the most common pathogen isolated from several crops. The aim of this study was to report the occurrence of F. graminearum sensu stricto on rye grains collected from field samples in Argentina and to determine the potential ability to produce deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEA). Based on morphological characteristics, the isolate was identified as F. graminearum sensu stricto. To confirm molecularly, portions of the RED and TRI genes were sequenced and showed 99% similarity with the F. graminearum sensu stricto sequences available in the NCBI database. The potential to produce DON, 15-acetyldeoxynivalenol (15-ADON) and ZEA was determined. Moreover, Koch´s postulates were carried out. To our knowledge, this is the first report of F. graminearum sensu stricto associated with rye kernels in Argentina.
FUNDING
This research was supported by FONCyT/PICT (0213/2015) and UNCPBA.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (31)
1.
Alvarez L.C., Azcarate M.P., Fernández Pinto V. 2009. Toxigenic potential of Fusarium graminearum sensu stricto isolates from wheat in Argentina. International Journal of Food Microbiology 135: 131–135. DOI: https://doi.org/10.1016/j.ijfo....
 
2.
Bennett J.W., Klich M. 2003. Mycotoxins. Clinical Microbiological Reviews 16: 497–516. DOI: https://doi.org/10.1128/CMR.16....
 
3.
Brumana J.F. 1938. Ficha Fitopatológica Nro. 002233. In: Ficheros y Archivos del Instituto de Patología Vegetal INTA. INTA Castelar, Buenos Aires, Argentina. (in Spanish).
 
4.
Bushuk W. 2001. Rye: Production, Chemistry, and Technology. 2nd ed. AACC, St. Paul, USA, 239 pp.
 
5.
Carranza J.M. 1971. Tostado del centeno y otros cereales. In: Jornadas Fitosanitarias: libro de Resúmenes, F-15. Facultad de Agronomía y Veterinaria, UBA, Buenos Aires, Argentina. (in Spanish).
 
6.
Castañares E., Dinolfo M.I., Del Ponte E.M., Pan D., Stenglein S.A. 2016. Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America. Plant Pathology 65: 930–939. DOI: https://doi.org/10.1111/ppa.12....
 
7.
Castañares E., Dinolfo M.I., Moreno M.V., Berón C., Stenglein S.A. 2013. Fusarium cerealis associated with barley seeds in Argentina. Journal of Phytopathology 161: 586–589. DOI: https://doi.org/10.1111/jph.12....
 
8.
Chandler E., Simpson D., Thomsett M., Nicholson P. 2003. Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis. Physiological and Molecular Plant Pathology 62 (6): 355–367. DOI: https://doi.org/10.1016/S0885-....
 
9.
Desjardins A.E. 2006. Fusarium mycotoxins: chemistry, genetics and biology. The American Phytopathological Society, St. Paul, USA, 260 pp.
 
10.
Desjardins A.E., Proctor R.H. 2007. Molecular biology of Fusarium mycotoxins. International Journal of Food Microbiology 119 (1–2): 47–50. DOI: https://doi.org/10.1016/j.ijfo....
 
11.
Dubin H.J., Gilchrist L., Reeves J., McNab A. 1997. Fusarium Head Scab: Global Status and Future Prospects. CIMMYT, El Batán, México, 130 pp.
 
12.
Fernández M.R., Selles F., Gehl D., DePauw R.M., Zentner R.P. 2005. Crop production factors associated with Fusarium head blight in spring wheat in southeast Saskatchewan. Crop Science 45: 1908–1916.
 
13.
Fernández Pinto V.E., Terminiello L.A., Basilico J.C., Ritieni A. 2008. Natural occurrence of nivalenol and mycotoxigenic potential of Fusarium graminearum strains in wheat affected by head blight in Argentina. Brazilian Journal of Microbiology 39 (1): 157–162. DOI: http://dx.doi.org/10.1590/S151....
 
14.
Góral T., Wisniewska H., Ochodzki P., Walentyn-Góral D. 2016. Higher Fusarium toxin accumulation in grain of winter triticale lines inoculated with Fusarium culmorum as compared with wheat. Toxins 8 (10): 301. DOI: https://doi.org/10.3390/toxins....
 
15.
Kiecana I., Mielniczuk E. 2010. Fusarium head blight of winter rye (Secale cereale L.). Acta Agrobotanica 63 (1): 129–135.
 
16.
Leslie J.F., Summerell B.A. 2006. The Fusarium laboratory manual. Blackwell Pub, Ames, Iowa, USA, 388 pp.
 
17.
Marasas W.F.O., Nelson P.E., Toussoun T.A. 1984. Toxigenic Fusarium Species: Identity and Mycotoxicology. The Pennsylvania State University Press, Pennsylvania, USA, 320 pp.
 
18.
Meng K., Wang Y., Yang P. 2010. Rapid detection and quantification of zearalenone producing Fusarium species bny targeting the zearalenone synthase gene PKS4. Food Control 21: 207–211. DOI: https://doi.org/10.1016/j.food....
 
19.
MINAGRI. 2018. Ministerio de Agroindustria, presidencia de la Nación. Available on: http://datosestimaciones.magyp.... [Accessed: September 5, 2018].
 
20.
Miralles D.J., González F.G., Abeledo L.G., Serrago R.A., Alzueta I., García G.A., de San Caledonio R.P., Lo Valvo P. 2014. Manual de trigo y cebada para el Cono Sur: procesos fisiológicos y bases de manejo. 1st ed. Orientación Gráfica Editora, Buenos Aires, Argentina, 56 pp. (in Spanish).
 
21.
O’Donnell K., Kistler H.C., Tacke B.K., Casper H.H. 2000. Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences 97 (14): 7905–7910. DOI: https://doi.org/10.1073/pnas.1....
 
22.
Placinta C.M., D’Mello J.P.F., Macdonald A.M.C. 1999. A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology 78 (1–2): 21–37. DOI: https://doi.org/10.1016/S0377-....
 
23.
Quarta A., Mita G., Haidukowski M., Logrieco A., Mulé G., Visconti A. 2006. Multiplex PCR assay for the identification of nivalenol, 3- and 15-acetyl-deoxynivalenol chemotypes in Fusarium. FEMS Microbiology Letters 259 (1): 7–13. DOI: https://doi.org/10.1111/j.1574....
 
24.
Reis E.M., Carmona M. 2002. Fusariosis del trigo: biología, epidemiología y estrategias para su manejo. 1st ed. BASF Argentina S.A., Buenos Aires, Argentina, 25 pp. (in Spanish).
 
25.
Reynoso M.M., Ramírez M.L., Torres A.M., Chulze S.N. 2011. Trichothecene genotypes and chemotypes in Fusarium graminearum strains isolated from wheat in Argentina. International Journal of Food Microbiology 145: 444–448. DOI: https://doi.org/10.1016/j.ijfo....
 
26.
Schöneberg T., Kibler K., Wettstein F.E., Bucheli T.D., Forrer H.R., Musa T., Mascher F., Bertossa M., Kellier B., Vogelgsang S. 2018. Influence of temperature, humidity duration and growth stage on the infection and mycotoxin production by Fusarium langsethiae and Fusarium poae in oats. Plant Pathology 68 (1): 173–184. DOI: https://doi.org/10.1111/ppa.12....
 
27.
Stenglein S.A., Balatti P.A. 2006. Genetic diversity of Phaeoisariopsis griseaola in Argentina as revealed by virulence and molecular markers. Physiological and Molecular Plant Pathology 68 (4–6): 158–167. DOI: https://doi.org/10.1016/j.pmpp....
 
28.
Stenglein S.A., Dinolfo M.I., Barros G., Bongiorno F., Chulze S.N., Moreno M.V. 2014. Fusarium poae: Pathogenicity and mycotoxin accumulation on selected wheat and barley genotypes at a single location in Argentina. Plant Disease 98 (12): 1733–1738. DOI: https://doi.org/10.1094/PDIS-0....
 
29.
Trigo Argentino. 2018. Informe Institucional de su Calidad. Available on: http://www.trigoargentino.com.....
 
30.
USDA. 2018. Available on: https://www.fas.usda.gov/data/... feedupdate-7 [Accessed: September 5, 2018].
 
31.
Xu X.M., Nicholson P., Thomsett M.A., Simpson D., Cooke B.M., Doohan F.M. 2008. Relationship between the fungal complex causing Fusarium head blight of wheat and environmental conditions. Phytopathology 98 (1): 69–78. DOI: https://doi.org/10.1094/PHYTO-....
 
eISSN:1899-007X
ISSN:1427-4345
Journals System - logo
Scroll to top