ORIGINAL ARTICLE
Plants containing cardiac glycosides showing antiphytoviral activity against Potato virus Y (PVYNTN) on tobacco plants
Simin Emamzadeh Yazdi 1, A-F
,  
Julia Mulabisana 2, A-C,E-F
,  
Gerhard Prinsloo 3, A,E-F
,  
Michele Cloete 2, A,E-F
,  
Quenton Kritzinger 1, A,E-F  
 
 
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1
Department of Plant and Soil Sciences, University of Pretoria, Pretoria, South Africa
2
Agricultural Research Council, Vegetable and Ornamental Plants, Roodeplaat, Pretoria, South Africa
3
Department of Agriculture and Animal Health, University of South Africa (UNISA), Florida Campus, South Africa
A - Research concept and design; B - Collection and/or assembly of data; C - Data analysis and interpretation; D - Writing the article; E - Critical revision of the article; F - Final approval of article
CORRESPONDING AUTHOR
Quenton Kritzinger   

Department of Plant and Soil Sciences, University of Pretoria, Lynnwood road, Hatfield, 0002 Pretoria, South Africa
Online publish date: 2018-12-17
Submission date: 2018-05-31
Acceptance date: 2018-11-28
 
Journal of Plant Protection Research 2018;58(4):397–403
KEYWORDS
TOPICS
ABSTRACT
The tuber necrotic strain of Potato virus Y (PVYNTN) causes widespread disease and has severe negative effects on the growth and yields of plants, especially those of the Solanaceae family. The consequences of residual toxicity and non-biodegradation of synthetic chemicals and pollution of the environment has led to investigations into new non-toxic and biological treatments to control plant viral diseases. Ethanolic extracts of Bowiea volubilis (bulbs), Cotyledon orbiculata (leaves), Gomphocarpus fruticosus (leaves), Merwilla plumbea (dry and fresh bulbs), Nerium oleander (leaves), and the fruits and leaves of Strophanthus speciosus, were evaluated against PVYNTN in vivo and in vitro. At a concentration of 20 mg · ml−1, ethanolic extracts of Strophanthus speciosus (leaves) and fruits (50 mg · ml−1) significantly reduced the expression of PVYNTN symptoms on tobacco plants in vitro without affecting the normal growth and development of the plant. Similarly, at 50 mg · ml−1, N. oleander, C. orbiculata and B. volubilis (fresh bulbs) and S. speciousus leaves at 20 mg · ml−1 extracts showed significant differences in PVYNTN symptoms in the in vivo experiment. Strophanthus speciosus leaf and fruit extracts showed significant inhibition in the in vitro and in vivo assays and demonstrated that S. speciosus has potential to be used as an antiphytoviral treatment.
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
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