ORIGINAL ARTICLE
Recombinants of PVY strains predominate among isolates from potato crop in Poland
 
More details
Hide details
1
Młochów Research Center, Plant Breeding and Acclimatization Institute – National Research Institute Platanowa 19, 05-831 Młochów, Poland
CORRESPONDING AUTHOR
Zhimin Yin
Młochów Research Center, Plant Breeding and Acclimatization Institute – National Research Institute Platanowa 19, 05-831 Młochów, Poland
Ewa Zimnoch-Guzowska
Młochów Research Center, Plant Breeding and Acclimatization Institute – National Research Institute Platanowa 19, 05-831 Młochów, Poland
 
Journal of Plant Protection Research 2012;52(2):2014–219
KEYWORDS
TOPICS
ABSTRACT
282 Potato virus Y (PVY) isolates collected from potato crops in northern and central Poland from 1995 to 2009 were characterized by serological and biological assays. From these, 112 isolates collected from 2006 to 2009 were additionally analyzed by one-step triplex Reverse Transcription-Polymeraze Chain Reaction (RT-PCR). Recombinants of PVY strains predominate among the isolates tested. Using one-step triplex RT-PCR most PVY N-Wi isolates were classified as subgroup PVY N Wi-P and most PVY N and/or PVY NTN isolates as the recombinant PVY NTN strain. A recombinant PVY NTN isolate (12/94) and two additional PVY N and/or PVY NTN isolates were not detected by one-step triplex RT-PCR. Twelve isolates were identified as the PVY O strain but PVY N , non-recombinant PVY NTN and PVY C strains were not found. Serological and biological assays of 144 isolates of PVY N-Wi strain showed that 100 isolates were the expected PVY O serotype with vein necrosis (VN) symptoms on tobacco. However, 10 isolates of the PVY N Wi-P subgroup exhibited vein clearing (VCl) on tobacco and 2 isolates of the PVY N N242 subgroup unexpectedly exhibited as the PVY N serotype. All the isolates of PVY N-Wi strain induced severe local lesions (LL) on Chenopodium amaranticolor . Out of 126 isolates of PVY NTN strain tested, 76 were typical PVY N serotype with VN on tobacco, but their reactions on C. amaranticolor were different: 13 isolates did not show symptoms, 23 isolates induced weak, and 40 isolates induced severe LL. The remaining isolates of PVY N-Wi , PVY N and/or PVY NTN or PVY O were serologically PVY N and PVY O positive or exhibited unpredictable serological and biological reactions
CONFLICT OF INTEREST
The authors have declared that no conflict of interests exist.
 
REFERENCES (40)
1.
Baldauf P.M., Gray S.M., Perry K.L. 2006. Biological and serological properties of Potato virus Y isolates in northeastern United States potato. Plant Dis. 90 (5): 559–566.
 
2.
Barker H., McGeachy K.D., Toplak N., Gruden K., Zel J., Browning I. 2009. Comparison of genome sequence of PVY isolates with biological properties. Am. J. Potato Res. 86 (3):227–238.
 
3.
Beczner L., Horváth H., Romhányi I., Förster H. 1984. Studies on the etiology of tuber necrotic ringspot disease in potato. Potato Res. 27 (4): 339–352.
 
4.
Blanchard A., Rolland M., Lacroix C., Kerlan C., Jacquot E. 2008. Potato virus Y: A century of evolution. Curr. Topics Virol. 7: 21–32.
 
5.
Boukhris-Bouhachem S., Djilani-Khouadja F., Fakhfakh H., Glais L., Tribodet M., Kerlan C. 2010. Incidence and characterization of Potato virus Y in seed potatoes in Tunisia. Potato Res. 53 (3): 151–166.
 
6.
Browning I., Charlet K., Chrzanowska M., Dědič P., Kerlan C., Kryszczuk A., Schubert J., Varveri C., Werkman A., Wolf I. 2004. What is PVY NTN? The reaction of potato cultivars to inoculation with a range of PVY isolates. p. 51–53. In: The 12th European Association for Potato Research Virology Section Meeting. Rennes, France, 13–19.06.2004.
 
7.
Chikh Ali M., Maoka T., Natsuaki K.T. 2007. A point mutation changes the serotype of a potato virus Y isolate; genomic determination of the serotype of PVY strains. Virus Genes 35 (2): 359–367.
 
8.
Chikh Ali M., Maoka T., Natsuaki T., Natsuaki K.T. 2010. PVY NTN-NW, a novel recombinant strain of Potato virus Y predominating in potato field in Syria. Plant Pathol. 59 (1): 31–41.
 
9.
Chrzanowska M. 1991. New isolates of the necrotic strain of Potato virus Y(PVYN) found recently in Poland. Potato Res. 34 (2): 179–182.
 
10.
Chrzanowska M. 1994. Differentiation of potato virus Y(PVY) isolates. Phytopathol. Pol. 8 (XX): 15–20.
 
11.
Chrzanowska M., Doroszewska T. 1997. Comparison between PVY isolates obtained from potato and tobacco plants grown in Poland. Phytopathol. Pol. 3: 63–71.
 
12.
Crosslin J.M., Hamm P.B., Hane D.C., Jaeger J., Brown C.R., Shiel P.J., Berger P.H., Thornton R.E. 2006. The occurrence of PVYO, PVYN, and PVYN:O strains of Potato virus Y in certified potato seed lot trials in Washington and Oregon. Plant Dis. 90 (8): 1102–1105.
 
13.
Glais L., Tribodet M., Kerlan C. 2002. Genomic variability in Potato potyvirus Y (PVY): evidence that PVY(N)Wand PVY(NTN)variants are single to multiple recombinants between PVY(O)and PVY(N)isolates. Arch. Virol. 147 (2): 363–378.
 
14.
Gray S., De Boer S., Lorenzen J., Karasev A., Whitworth J., Nolte P., Singh R., Boucher A., Xu H. 2010. Potato virus Y: an evolving concern for potato crops in the United States and Canada. Plant Dis. 94 (12): 1384–1397.
 
15.
Hu X., Karasev A.V., Brown C.J., Lorenzen J.H. 2009a. Sequence characteristics of potato virus Y recombinants. J. Gen. Virol. 90 (12): 3033–3041.
 
16.
Hu X., Meacham T., Ewing L., Gray S.M., Karasev A.V. 2009b. A novel recombinant strain of Potato virus Y suggests a new viral genetic determinant of vein necrosis in tobacco. Virus Res. 143 (1): 68–76.
 
17.
Hull R. 2009. Comparative Plant Virology. 2nd ed. Elsevier, 376 pp.
 
18.
Karasev A.V., Nikolaeva O.V., Hu X., Sielaff Z., Whitworth J., Lorenzen J.H., Gray S.M. 2010. Serological properties of ordinary and necrotic isolates of Potato virus Y: a case study of PŁYN misidentification. Am. J. Potato Res. 87 (1): 1–9.
 
19.
Kaliciak A., Syller J. 2009. Aphid transmissibility of genetically different isolates of Potato virus Y and susceptibility of weeds to virus infection. Biul. IHAR 253: 285–295.
 
20.
Kerlan C. 2003/4. Evolution in Potato virus Y: from recombination in the genome to emergence and spreading of variants. Potato Res. 46 (3–4): 184.
 
21.
Kerlan C. 2006. Potato virus Y. AAB descriptions of plant viruses no 414. From: http://www.dpvweb.net/dpv/show....
 
22.
Le Romancer M., Kerlan C., Nedellec M. 1994. Biological characterization of various geographical isolates of Potato virus Y inducing superficial necrosis on potato tubers. Plant Pathol. 43 (1): 138–144.
 
23.
Lorenzen J., Nolte P., Martin D., Pasche J.S., Gudmestad N.C. 2008. NE-11 represents a new strain variant class of Potato virus Y. Arch. Virol. 153 (3): 517–525.
 
24.
McDonald J.G., Singh R.P. 1996a. Host range, symptomology, and serology of isolates of Potato virus Y (PVY) that share properties with both the PŁYN and PVY Ostrain groups. Am. Potato J. 73 (7): 309–315.
 
25.
McDonald J.G., Singh R.P. 1996b. Response of potato cultivars to North American isolates of PVY NTN. Am. Potato J. 73 (7): 317–323.
 
26.
Menzel W., Jelkmann W., Maiss E. 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. J. Virol. Methods 99 (1–2): 81–92.
 
27.
Nie X., Singh R.P. 2002. A new approach for the simultaneous differentiation of biological and geographical strains of Potato virus Y by uniplex and multiplex RT-PCR. J. Virol. Methods 104 (1): 41–54.
 
28.
Nie X., Singh R.P. 2003. Evolution of North American PVY(NTN)strain Tu660 from local PVY(N)by mutation rather than recombination. Virus Genes 26 (1): 39–47.
 
29.
Nie X. 2010. Recent research progresses on Potato virus Y in Canada. Am. J. Potato Res. 87 (1): 136.
 
30.
Nie B., Singh M., Sullivan A., Singh R.P., Xie C., Nie X. 2011. Recognition and molecular discrimination of severe and mild PVYO variants of Potato virus Y in potato in New Brunswick, Canada. Plant Dis. 95 (2): 113–119.
 
31.
Ogawa T., Tomitaka Y., Nakagawa A., Ohshima K. 2008. Genetic structure of a population of Potato virus Y inducing potato tuber necrotic ringspot disease in Japan; comparison with North American and European populations. Virus Res. 131 (2): 199–212.
 
32.
Piche L.M., Singh R.P., Nie X., Gudmestad N.C. 2004. Diversity among potato virus Y isolates obtained from potatoes grown in the United States. Phytopathology 94 (12): 1368–1375.
 
33.
Ramírez-Rodríguez V.R., Frías-Treviño G., Aviña-Padilla K., Martínez-Soriano J.P. 2009. Presence of necrotic strains of Potato virus Y in Mexican potatoes. Virol. J. 6, p. 48.
 
34.
Rigotti S., Gugerli P. 2007. Rapid identification of potato virus Y strains by one-step triplex RT-PCR. J. Virol. Methods 140 (1–2): 90–94.
 
35.
Rigotti S., Balmelli C., Gugerli P. 2011. Census report of the Potato virus Y(PVY) population in Swiss seed potato production in 2003 and 2008. Potato Res. 54 (2): 105–117.
 
36.
Schubert J., Fomitcheva V., Sztangret-Wiśniewska J. 2007. Differentiation of Potato virus Y strains using improved sets of diagnostic PCR-primers. J. Virol. Methods 140 (1–2): 66–74.
 
37.
Singh R. P., Valkonen J.P., Gray S.M., Boonham N., Jones R.A., Kerlan C., Schubert J. 2008. Discussion paper: The naming of Potato virus Y strains infecting potato. Arch. Virol. 153 (1): 1–13.
 
38.
Syller J. 2001. The Enzyme-Linked Immunosorbent Assay (ELISA) procedure. p 21–23. In: „Monografie i Rozprawy Naukowe 10a/2001” (E. Zimnoch-Guzowska, J. Syller, M. Sieczka, eds.). IHAR, Radzików, 131 pp.
 
39.
Tribodet M., Glais L., Kerlan C., Jacquot E. 2005. Characterization of Potato virus Y (PVY) molecular determinants involved in the vein necrosis symptom induced by PŁYN isolates in infected Nicotiana tabacum cv. Xanthi. J. Gen. Virol. 86 (7): 2101–2105.
 
40.
Visser J.C., Bellstedt D.U. 2009. An assessment of molecular variability and recombination patterns in South African isolates of Potato virus Y. Arch. Virol. 154 (12): 1891–1900.
 
eISSN:1899-007X
ISSN:1427-4345