Biological control of tomato verticillium wilt disease by Talaromyces flavus
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Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515/775, Tehran, Iran
Plant Disease Research Department, Iranian Research Institute of Plant Protection, P.O. Box 1452, Tehran 19395, Iran
Department of Microbiology, College of Science, Tehran University, P.O. Box 1452, Tehran, Iran
Corresponding author
Naraghi Laleh
Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, P.O. Box 14515/775, Tehran, Iran
Journal of Plant Protection Research 2010;50(3):360-365
In this study, Talaromyces flavus a fungal antagonist, was isolated from soil samples collected from tomato fields in Tehran and the Western Azarbayjan provinces of Iran. Antagonistic effects of T. flavus isolates against Verticillium albo-atrum (V. a.-a.), the causal agent of tomato wilt disease were investigated under laboratory and greenhouse conditions. Soil samples from tomato fields in the Varamin and Uremia regions of Tehran and the Western Azarbayjan provinces respectively, were cultured on selective medium. T. flavus colonies were recovered after three weeks. In the laboratory experiments, antagonistic effects of volatile and non-volatile extracts of T. flavus isolates on V. a.-a. growth were investigated. Among isolates, five that caused higher growth inhibition of V. a.-a. were selected for greenhouse experiments. In the greenhouse, first inoculum of V. a.-a. and treatments affected by T. flavus isolates were prepared. For comparison of the infection index in treatments, the greenhouse experiment was performed with a split plot arranged in a randomized complete block design with four replications. Result of greenhouse experiments on different types of T. flavus treatments indicated that there was no significant difference among them. However, among five T. flavus isolates, the most effective one was Tf-To-V-24 and Tf-To-U-36. In the experiment on the interaction between different T. flavus treatments and T. flavus isolates, a minimum infection index was observed when both soil and seed were treated with Tf-To-V-31. The overall results of this study show that it may be possible to manage tomato Verticillium wilt disease effectively by T. flavus.
The authors have declared that no conflict of interests exist.
Adebanjo A., Bankole S.A. 2004. Evaluation of some fungi and bacteria for biocontrol of anthracnose disease of cowpea. J. Basic Microbiol. 44 (1): 3–9.
Alemzadeh Ansari N., Mamghani R. 2008. A study on adaptation of tomato ecotypes from northern latitudes under southern Iran conditions. J. Appl. Hort. 10: 29–33.
Aminaee M. M., Mansoori B., Ershad D. 2006. A study on Verticillium wilt of tomato in Kerman province. In: Proceedings of the 17th Iranian Plant Protection Congress (M. Abbasi, F. Aliabadi, eds.). University of Tehran, Karaj, p. 163.
Bal U., Abak K. 2007. Haploidy in tomato (Lycopersicon esculentum Mill.): a critical review. J. Euphytica 158 (1–2): 1–9.
Bourbos V.A., Skoudridakis M.T. 1996. Soil solarization for the control of Verticillium wilt of greenhouse. Phytoparasitica 24 (4): 277–280.
Brown A.E. 1987. Activity of glucanases of Zygorrhynchus moelleri in relation to antagonism against some soil borne plant pathogenic fungi. J. Phytopathol. 120 (4): 298–309.
Cherif M., Arfaoni A., Khaiem A. 2007. Phenolic compounds and their role in biocontrol and resistance of chickpea to fungal pathogenic attacks. Tunisian J. Plant Protect. 2: 7–21.
Chet I., Baker R. 1981. Isolation and biocontrol potential of Trichoderma hamatum from soil naturally suppressive to Rhizoctonia solani. Phytopathology 71: 286–290.
Christen A.A. 1981. A selective medium for isolating Verticillium albo-atrum from soil. Phytopathology 72: 47–49.
El-Tarabily K.A., Soliman M.H., Nassar A.H., Al-Hassani H.A., Sivasithamparam K., Mc Kenna F., Hardy G.E. 2000. Biological control Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol. 49: 573–583.
Eziashi E.L., Uma N.U., Adekunle A.A., Ariede C.E. 2006. Effect of metabolites produced by Trichoderma species against Ceratocystis paradoxa in culture medium. African J. Biotech. 5 (9): 703–706.
Fahima T., Henis Y. 1997. Increasing of Trichoderma hamatum and Talaromyces flavus on the root of safe and useful hosts. p. 296–322. In: “Biological Control of Soil-Borne Plant Pathogens Hornby” (A. Alavi, A. Ahoonmanesh, eds.). Tehran, Iran.
Giotis C., Markelou E., Theodoropoulou A., Toufexi E., Hodson R., Shotton P., Shiel R., Cooper J., Leifert C. 2009. Effect of soil amendments and biological control agents (BCAs) on soil-borne root disease caused by Pyrenochaetu lycopersici and Verticillium albo-atrum in organic greenhouse tomato production systems. Eur. J. Plant Pathol. 123: 387–400.
Hanafi A. 2003. Integrated Production and Protection in Greenhouse Tomato in Morocco. p. 192–197. In: “Tomate Sous Abri” Scientific Publication of CTIFL. 2003. Editions Centre Technique Interprofessionnel des Fruits et Légumes, 232 pp.
Hutson R.A., Smith I.M. 1982. The response of tomato seedling roots to infection by Verticillium albo-atrum or Fusarium oxysporum f. sp. lycopersici. Ann. Appl. Biol. 102 (1): 89–97.
Jones J.P., Gilreath J.P., Overman A.J. 1995. Control of soil-borne diseases of mulched tomato by fumigation. Proc. Fla. State Hort. Soc. 108: 201–203.
Josh D., Hooda K.S., Bhatt J.C., Mina B.L., Gupta H.S. 2009. Suppressive effects of composts on soil-borne and foliar diseases of french bean in the field in the Western Indian Himalayas. Crop Protect. 28 (7): 608–615.
Kim K.K., Fravel D.R. 1990. Glucose oxidase as the antifungal principle of talaron from Talaromyces flavus. Can. J. Microbiol. 36 (11): 760–764.
Kim J.T., Park I.H., Lee H.B., Hahm Y.I., Yu S.H. 2001. Identification of Verticillium dahliae and Verticillium albo-atrum causing wilt of tomato in Korea. Plant Pathol. J. 17 (4): 222–226.
Kulikov S., Alimova F., Zakharova N., Nemtsev S., Varlamov V. 2006. Biological preparations with different mechanisms of action for protecting tomato against fungal diseases. Appl. Biochem. Microbiol. 42: 77–83.
Madi L., Katan T., Katan J., Henis Y. 1997. Biological control of Sclerotium rolfsii and Verticillium dahliae by Talaromyces flavus is mediated by different mecchanisms. Phytopathology 87: 1054–1060.
Mansoori B., Smith C.J. 2005. Elicitation of ethylene by Verticillium albo-atrum phytotoxins in tomato. J. Phytopathol. 153 (3): 143–149.
Marois J.J., Jahnson S.A., Dunn M.T., Papavizas G.C. 1982. Biological control of Verticillium wilt of eggplant in the field. Plant Dis. 6 (12): 1166–1168.
Marois J.J., Fravel D.R., Papavizas G.C. 1984. Ability of Talaromyces flavus to occupy the rhizosphere. Soil Bio. Biochem. 16 (4): 387–390.
Matta A., Garibadli A. 1997. Control of Verticillium wilt of tomato by preinoculation with avirulent fungi. Eur. J. Plant Pathol. 83 (1): 457–462.
McLaren D.L., Huang H.C., Rimmer S.R. 1982. Hyphal interactions occurring between Sclerotinia sclerotiorum and Penicillium vermiculatum. Can. J. Plant Pathol. 4, p. 308.
Menendez A.B., Godeas A. 1998. Biological control of Sclerotinia sclerotiorum attacking soybean plants: degradation of the cell wall of this pathogen by Trichoderma harzianum. Mycopathologia 142 (3): 153–160.
Murrary F.R., Liewellyn D.J., Peacock W.J., Dennis E.S. 1997. Isolation of the glucose oxidase gene from Talaromyces flavus and characterization of its role in the biocontrol of Verticillium dahliae. Curr. Genet. 32 (5): 367–375.
Nagtzaam M.P., Bollen G.J. 1997. Colonization of roots of eggplant and tomato by Talaromyces flavus from coated seed. Soil Biol. Biochem. 29 (9–10): 1499–1507.
Paplomatas E.J., Elena K., Tsagkarakou A. 1999. Screening Tomato, Cucumber, Watermelon and Melon Rootstocks for Resistance to Verticillium dahliae. EPPO/MPU Conference on Cucurbitaceous and Solanaceous Vegetable Diseases in the Mediterranean Area. 11–14 October 1999. Kerkyra, Greece, p. 30.
Paternotte S.J., Van Kesteren H.A. 1993. A new aggressive strain of Verticillium albo-atrum in Verticillium resistant cultivars of tomato in the Netherlands. Eur. J. Plant Pathol. 99 (3): 169–172.
Pegg G.F., Young D.H. 1982. Purification and characterization of chitinase enzymes from healthy and Verticillium albo-atrum infected tomato plants, and from V. albo-atrum. Physiol. Plant Pathol. 21 (3): 389–398.
Pohronezny K. 1991. Verticillium wilt. p. 12–24. In: “Compendium of Tomato Diseases” (J.B. Jones, J.P. Jones, R.E. Stall, T.A. Zitter, eds.). APS Press, St. Paul, MN, USA.
Proksa B., Adamcova J., Fuska J. 1992. 2-methylsorbic acid, an antifungal metabolite of Penicillium vermiculatum. J. Appl. Microbiol. Biotech. 37 (4): 443– 445.
Rodriguez M.A., Cabrera G., Godeas A. 2006. Cyclosporine a from a nonpathogenic Fusarium oxysporum suppressing Sclerotinia sclerotiorum. J. Appl. Microbiol.100 (3): 575–586.
Sahebani N., Hadavi N. 2009. Induction of H2O2 and related enzymes in tomato roots infected with root-knot nematode (Meloidogyne javanica) by several chemical and microbial elicitors. Biocontrol Sci. Technol. 19 (3): 301–313.
Soytong K., Ratanacherdchai K. 2005. Application of mycofungicide to control late blight of tomato. J. Agric. Technol. 1: 19–32.
Tjamos E.C. 1991. Recovery of olive tree with Verticillium dahliae after individual application of soil solarization in stablished olive orchards. Plant Dis. 75 (6): 557–562.
Tjamos E.C., Fravel D.R. 1997. Distribution and establishment of the biocontrol fungus Talaromyces flavus in soil and on roots of solanaceous crops. Crop Protect. 16 (2): 135–139.
Tjamos E.C., Paplomatas E.J. 1987. Effect of soil solarization on the survival of fungal antagonists of Verticillium dahliae. EPPO Bull. 17 (4): 645–653.
Vidhyasekaren P. 2004. Concise Encyclopedia of Plant Pathology. 1st ed. Haworth Press Inc., Binghamton, NY.
Wikins T.A, Rajasekaran K., Anderson D.M. 2000. Cotton biotechnology. Critical Rev. Plant Sci. 19: 511–550.
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