Recombinants of PVY strains predominate among isolates from potato crop in Poland
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Młochów Research Center, Plant Breeding and Acclimatization Institute – National Research Institute Platanowa 19, 05-831 Młochów, Poland
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Zhimin Yin
Młochów Research Center, Plant Breeding and Acclimatization Institute – National Research Institute Platanowa 19, 05-831 Młochów, Poland
Ewa Zimnoch-Guzowska
Młochów Research Center, Plant Breeding and Acclimatization Institute – National Research Institute Platanowa 19, 05-831 Młochów, Poland
Journal of Plant Protection Research 2012;52(2):2014-219
282 Potato virus Y (PVY) isolates collected from potato crops in northern and central Poland from 1995 to 2009 were characterized by serological and biological assays. From these, 112 isolates collected from 2006 to 2009 were additionally analyzed by one-step triplex Reverse Transcription-Polymeraze Chain Reaction (RT-PCR). Recombinants of PVY strains predominate among the isolates tested. Using one-step triplex RT-PCR most PVY N-Wi isolates were classified as subgroup PVY N Wi-P and most PVY N and/or PVY NTN isolates as the recombinant PVY NTN strain. A recombinant PVY NTN isolate (12/94) and two additional PVY N and/or PVY NTN isolates were not detected by one-step triplex RT-PCR. Twelve isolates were identified as the PVY O strain but PVY N , non-recombinant PVY NTN and PVY C strains were not found. Serological and biological assays of 144 isolates of PVY N-Wi strain showed that 100 isolates were the expected PVY O serotype with vein necrosis (VN) symptoms on tobacco. However, 10 isolates of the PVY N Wi-P subgroup exhibited vein clearing (VCl) on tobacco and 2 isolates of the PVY N N242 subgroup unexpectedly exhibited as the PVY N serotype. All the isolates of PVY N-Wi strain induced severe local lesions (LL) on Chenopodium amaranticolor . Out of 126 isolates of PVY NTN strain tested, 76 were typical PVY N serotype with VN on tobacco, but their reactions on C. amaranticolor were different: 13 isolates did not show symptoms, 23 isolates induced weak, and 40 isolates induced severe LL. The remaining isolates of PVY N-Wi , PVY N and/or PVY NTN or PVY O were serologically PVY N and PVY O positive or exhibited unpredictable serological and biological reactions
The authors have declared that no conflict of interests exist.
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