Control of postharvest rots of banana fruits by conidia and culture filtrates of Trichoderma asperellum
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Department of Science Laboratory Technology, Federal Polytechnic, P.M.B. 231 Ede. Osun State, Nigeria
Department of Botany and Microbiology, University of Ibadan, UI Post Office, Ibadan. Nigeria
Germplasm Health Unit, International Institute of Tropical Agriculture, Ibadan. Nigeria
Abolade Ayodeji Adebesin
Department of Science Laboratory Technology, Federal Polytechnic, P.M.B. 231 Ede. Osun State, Nigeria
Chris Adegboyega Odebode
Department of Botany and Microbiology, University of Ibadan, UI Post Office, Ibadan. Nigeria
Journal of Plant Protection Research 2009;49(3):302–308
Banana fruits are highly perishable and prone to microbial infection that cause significant damage. Fungicides and pesticides that are used to control this infection are toxic to man and animals, hence there is the need for environmentally friendly control measures of fruit rot pathogens. Simultaneous inoculation of fruits with Trichoderma species and rot pathogens resulted in rot on the fingers, but rot produced by T.asperellum NG-T161 alone or in combination with the pathogens was reduced, compared to rot produced by the pathogens alone. Treatment of fruits with conidia and culture filtrates of T. asperellum NG-T161 for 30 min prior to inoculation with the pathogens provided a better control than their simultaneous application. Only Trichoderma species were recovered on plated portions of rotted tissues from inoculations with the pathogens and the antagonists on the fruits. At 50% (v/v) the filtrates inhibited the mycelial growth of Fusarium oxysporum and Colletotrichummusae by 49.7 and 60.3% respectively but Lasiodiplodia theobromae was not inhibited. T. asperellum strains were found to be mycoparasitic on banana fruit rot pathogens. Conidia and culture filtrates of T. asperellum NG-T161 controlled the rot on banana fruits. It could be developed into a biopesticide for the control of postharvest banana fruit rot pathogens.
The authors have declared that no conflict of interests exist.
Adebesin A.A., Odebode A.C., Ayodele M.A. 2005. Fungal species associated with market fruits of Musa spp. in South Western Nigeria. Bull. Sci. Assoc. Nigeria 26: 299–305.
Adejumo T.O., Ikotun T., Florini D.A. 1999. Biological control of Protomycopsis phaseoli, the causal agent of leaf smut of cowpea. J. Phytopathol. 147: 371–375.
Bastos C.N. 1988. Resultados preliminaries sobre a eficacia de TrichodermaViride no controle da vassoura-de bruxa (Crinipellis perniciosa) do cacauerio. Fitopathol. Brasileira 13:340–343 (Abstract).
Benitez, T., Rincon, A.M., Limon M.C., Codon, A. C. 2004. Biocontrol mechanisms of Trichoderma strains. Intern. Microbiol.7(4): 249–260.
De Lapeyre De Bellaire L., Dubois C. 1997. Distribution of thiabendazole resistant Colletotrichum musae isolates from Guadeloupe banana plantation. Plant Dis. 81: 1378–1383.
Elad Y. 1994. Biological control of grape gray mold by Trichoderma harzianum. Crop Prot.13: 35–38.
Finlay A.R., Brown A.E. 1993. The relative importance of Colletorichum musaeas a crown-rot pathogen on Windward Island bananas. Plant Pathol. 42: 67–74.
Ghisalberti E.L., Sivasithamparam K. 1991. Antifungal antibiotics produced by Trichoderma speciesSoil Biochem. 23:1011–1020.
Griffee P.J. 1976. Pathogenicity of some fungi isolated from diseased crowns of banana hands.Phytopathol. Z. 85: 206–216.
Howell C.R. 2003. Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Dis. 87: 4–10.
Jackson A.M., Whipps J.M., Lynch J.M., Bazin M. J. 1991. Effects of some Carbon and Nitrogen sources on spore germination, production of biomass and antifungal metabolites by species of Trichoderma and Gliocladium virens antagonistic to Sclerotium cepivorum. Biocontrol Sci. Technol. 1: 43–51.
Johanson A., Blazquez B. 1992. Fungi associated with banana crown rot on field packed fruit from Windward Islands and assessment of their sensitivity to the fungicides Thiabendazole, Prochloraz and Imazalil. Crop Prot. 11: 79–83.
Kesiang G., Xiaoguag L., Yonghong L., Tianbo Z., Shulliang W. 2002. Potential of Trichoderma harzianum and T.atroviride to control Botryosphaeria berengeriana f. sp. Piricola, the cause of Apple Ring Rot. J. Phytopathol. 150: 271–276.
Krauss U., Bidwell R., Ince J. 1998.Isolation and preliminary evaluation of mycoparasites as biocontrol agents of crown rot of banana. Biological control – theory and application 13: 111–119.
Mortuza M.G., Ilag L.L. 1999. Potential for biocontrol of Lasiodiplodia theobromae(Pat). Griff and Maubi. in banana fruits by Trichoderma species. Biol. Control15: 235–240.
Odebode A.C. 2006. Control of postharvest pathogens of fruits by culture filtrate from antagonistic fungi. J. Plant Protection Res. 46: 1–5.
Odebode A.C., Sobowale A. 2001. Antagonistic activity of fungal flora isolated from pepper phylloplane on post-harvest pathogens of pepper (Capsicum annuum). Acta Phytopathol. Entomol. Hungarica36: 287–292.
Okigbo R.N., Ikediugwu F.E.O. 2000. Studies in biological control of postharvest rot in Yams (Dioscorea spp.) using Trichoderma viride. J. Phytopathol. 148: 351–355.
Ozbay N., Newman, S.E. 2004. Biological control with Trichoderma spp. with emphasis on T. harzianum. Pakistan J. Biol. Sci. 7:478–484.
Papavivas G.C. 1985. Trichoderma and Gliocladium: Biology, ecology and potential for biocontrol. Ann. Rev. Phytopathol. 23: 23–54.
Roberts R.G. 1990 Postharvest biological control of gray mold of apples by Cryptococcus laurentii. Phytopathology 80: 526–530.
Shillingford C.A. 1976. Occurrence of banana fruit rot fungi in Jamaican boxing plants. Plant Dis. Reporter 60: 788–793.
Slabaugh W. R., Grove M. D. 1982. Post-harvest diseases of banana and their control. Plant Dis. 66: 746–750.
Sobowale A.A., Cardwell K.F., Odebode A.C., Bandyopadhyay R., Jonathan S.G. 2005. Growth inhibition of Fusarium verticillioides (Sacc.) Nirenberg by isolates of Trichoderma pseudokoningii strains from maize plant parts and its rhizosphere. J. Plant Protection Res. 45: 249–265.
Tronsmo A. Dennis, C. 1977. The use of Trichoderma species to control strawberry fruit rots. Neth. J. Plant. Pathol.83: 449–455.